LOCUS       sequence-254651-       11063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for 
            NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to 
            NCBI ABO15149.1)-NLS-UGI-P2A-EGFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Grunewald J, Zhou R, Iyer S, Lareau CA, Garcia SP, Aryee MJ, Joung 
            JK
  TITLE     CRISPR DNA base editors with reduced RNA off-target and self-editing
            activities.
  JOURNAL   Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6.
  PUBMED    31477922
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6."
COMMENT     Sequence Label: Target-AID (pRZ762)
FEATURES             Location/Qualifiers
     source          1..11063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(493..1284,1285..1353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1116..1135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1354..1458)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1489..1868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1870..2147
                     /label=chicken beta-actin promoter
     intron          2148..3164
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3088..3110
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3172..3191
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3257..3266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3269..3289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3314..3340,3341..3343,3344..7417)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3314..7417
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7430..7450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7658..7723
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8357..8377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8384..8635
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8654..8710
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8711..8713,8714..8716,8717..9430)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8711..8713,8714..8716,8717..9427)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8756..8777)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9017..9036)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9364..9385
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9517..9536)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9582..9637
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9636..9655)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9998..10014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9998..10014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10011..10033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10022..10038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10046..10052,10053..10070,10071..10076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10091..10112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10171..10366
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10217..10352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10372..10506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10409..10428)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10463..10482
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10574..10591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10825..10844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cskkcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
       61 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat
     1501 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1561 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1621 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1681 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1741 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1801 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1861 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     1921 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     1981 gggggggggg gcgcgcgccr ggsggggsgg ggsggggsgg ggggsggggs ggggsgrggc
     2041 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     2101 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     2161 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     2221 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     2281 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     2341 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     2401 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     2461 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2521 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2581 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2641 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2701 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2761 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2821 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2881 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2941 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     3001 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     3061 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     3121 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     3181 tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg taccgcgggc
     3241 ccgggatcca ccggtcgcca ccatggcacc gaagaagaag cgtaaagtcg gaatccacgg
     3301 agttcctgcg gcaatggaca agaagtactc cattgggctc gctatcggca caaacagcgt
     3361 cggttgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
     3421 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
     3481 ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
     3541 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
     3601 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
     3661 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
     3721 tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct
     3781 cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
     3841 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
     3901 cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct
     3961 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
     4021 cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt
     4081 cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga
     4141 caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct
     4201 gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc
     4261 gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa
     4321 ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa
     4381 aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat
     4441 taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga
     4501 agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct
     4561 gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa
     4621 cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc
     4681 ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg
     4741 gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac
     4801 taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga
     4861 gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa
     4921 gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa
     4981 ccggaaagtt accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga
     5041 ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga
     5101 tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct
     5161 tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt
     5221 gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata
     5281 tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg
     5341 aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt
     5401 gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca
     5461 gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg
     5521 aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc
     5581 cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa
     5641 cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct
     5701 taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct
     5761 gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta
     5821 cgacgtggat catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt
     5881 gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt
     5941 caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa
     6001 gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt
     6061 catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga
     6121 ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat
     6181 tactctgaag tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag
     6241 agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc
     6301 acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta
     6361 cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta
     6421 cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga
     6481 gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa
     6541 gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa
     6601 aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag
     6661 cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc
     6721 tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa
     6781 actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa
     6841 aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat
     6901 taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag
     6961 tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt
     7021 gtatctggcc agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca
     7081 gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt
     7141 ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa
     7201 gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac
     7261 caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta
     7321 cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta
     7381 tgaaacaaga atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa
     7441 gaggaaggtg ggtggaggag gtaccggcgg tggaggctca gcagaatacg tacgagctct
     7501 gtttgacttc aatgggaatg acgaggagga tctccccttt aagaagggcg atattctccg
     7561 catcagagat aagcccgaag aacaatggtg gaatgccgag gatagcgaag ggaaaagggg
     7621 catgattctg gtgccatatg tggagaaata ttccggtgac tacaaagacc atgatgggga
     7681 ttacaaagac cacgacatcg actacaaaga cgacgacgat aaatcaggga tgacagacgc
     7741 cgagtacgtg cgcattcatg agaaactgga tatttacacc ttcaagaagc agttcttcaa
     7801 caacaagaaa tctgtgtcac accgctgcta cgtgctgttt gagttgaagc gaaggggcga
     7861 aagaagggct tgcttttggg gctatgccgt caacaagccc caaagtggca ccgagagagg
     7921 aatacacgct gagatattca gtatccgaaa ggtggaagag tatcttcggg ataatcctgg
     7981 gcagtttacg atcaactggt attccagctg gagtccttgc gctgattgtg ccgagaaaat
     8041 tctggaatgg tataatcagg aacttcgggg aaacgggcac acattgaaaa tctgggcctg
     8101 caagctgtac tacgagaaga atgcccggaa ccagatagga ctctggaatc tgagggacaa
     8161 tggtgtaggc ctgaacgtga tggtttccga gcactatcag tgttgtcgga agattttcat
     8221 ccaaagctct cataaccagc tcaatgaaaa ccgctggttg gagaaaacac tgaaacgtgc
     8281 ggagaagtgg agatccgagc tgagcatcat gatccaggtc aagattctgc ataccactaa
     8341 gtctccagcc gttggtccca agaagaaaag aaaagtcggt accatgacca acctttccga
     8401 catcatagag aaggaaacag gcaaacagtt ggtcatccaa gagtcgatac tcatgcttcc
     8461 tgaagaagtt gaggaggtca ttgggaataa gccggaaagt gacattctcg tacacactgc
     8521 gtatgatgag agcaccgatg agaacgtgat gctgctcacg tcagatgccc cagagtacaa
     8581 accctgggct ctggtgattc aggactctaa tggagagaac aagatcaaga tgctaggagg
     8641 cggtggaagc ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     8701 tcctggaccg atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     8761 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     8821 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     8881 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     8941 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     9001 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     9061 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     9121 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     9181 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     9241 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     9301 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     9361 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     9421 gtacaagtga agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     9481 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     9541 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     9601 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     9661 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     9721 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     9781 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     9841 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     9901 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     9961 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    10021 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10081 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10141 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
    10201 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10261 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10321 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
    10381 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10441 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10501 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10561 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10621 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10681 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10741 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10801 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10861 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10921 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10981 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatcmg
    11041 strrsywkyg tsywsrrwcc mmc
//
