LOCUS       sequence-25467-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC89
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..50)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    89..136
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(322..338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(322..338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(335..357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    346..362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(370..376,377..394,395..400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    415..436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(553..570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcttctcctt tactcatttt ttctaccggt actttgcgtt tctttttagg tgccgtcatt
       61 ttttctgagc tcggtacccg gggatcttga agttcctatt ccgaagttcc tattctctag
      121 aaagtatagg aacttcagag cgcttttgaa gctagaacta gtggatcccc cgggctgcag
      181 gcatgcaagc ttatttcatt tccaagttgt tagcgtatcc atcgttgtga gtgatttcag
      241 ccatttttaa gctctgaaaa tttaaatgag aatagttagt tagtaagatc atgtttaaac
      301 ttacagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      661 gaacatgtga gca
//
