LOCUS       sequence-254673-       10730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for hA3A(N57G)-XTEN 
            linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Grunewald J, Zhou R, Iyer S, Lareau CA, Garcia SP, Aryee MJ, Joung 
            JK
  TITLE     CRISPR DNA base editors with reduced RNA off-target and self-editing
            activities.
  JOURNAL   Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6.
  PUBMED    31477922
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 
            10.1038/s41587-019-0236-6."
COMMENT     Sequence Label: eA3A-BE3 (pRZ206)
FEATURES             Location/Qualifiers
     source          1..10730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(493..1284,1285..1353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1116..1135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1354..1458)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1489..1868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2148..3164
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3088..3110
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3172..3191
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3257..3266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3263..3859
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHGQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             join(3908..7306,7307..7309,7310..7906,7907,7908..7910,
                     7911..7912,7913..7915,7916..8008)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3908..3931,3932..3934,3935..8008)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8021..8269
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8282..8302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8321..8377
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8378..8380,8381..8383,8384..9097)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8378..8380,8381..8383,8384..9094)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8423..8444)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8684..8703)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9031..9052
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9184..9203)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9249..9304
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9303..9322)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9665..9681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9665..9681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9678..9700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9689..9705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9713..9719,9720..9737,9738..9743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9758..9779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9838..10033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9884..10019
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10039..10173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10076..10095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10130..10149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10241..10258)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10492..10511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cskkyagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
       61 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat
     1501 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1561 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1621 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1681 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1741 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1801 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1861 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     1921 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     1981 gggggggggg gcgcgcgccg gggggggggg gggggggggg ggggsggggs ggggsgrggs
     2041 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     2101 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     2161 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     2221 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     2281 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     2341 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     2401 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     2461 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2521 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2581 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2641 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2701 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2761 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2821 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2881 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2941 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     3001 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     3061 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     3121 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     3181 tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg taccgcgggc
     3241 ccgggatcca ccggtcgcca ccatggaagc cagcccagca tccgggccca gacacttgat
     3301 ggatccacac atattcactt ccaactttaa caatggcatt ggaaggcata agacctacct
     3361 gtgctacgaa gtggagcgcc tggacaatgg cacctcggtc aagatggacc agcacagggg
     3421 ctttctacac ggccaggcta agaatcttct ctgtggcttt tacggccgcc atgcggagct
     3481 gcgcttcttg gacctggttc cttctttgca gttggacccg gcccagatct acagggtcac
     3541 ttggttcatc tcctggagcc cctgcttctc ctggggctgt gccggggaag tgcgtgcgtt
     3601 ccttcaggag aacacacacg tgagactgcg tatcttcgct gcccgcatct atgattacga
     3661 ccccctatat aaggaggcac tgcaaatgct gcgggatgct ggggcccaag tctccatcat
     3721 gacctacgat gaatttaagc actgctggga cacctttgtg gaccaccagg gatgtccctt
     3781 ccagccctgg gatggactag atgagcacag ccaagccctg agtgggaggc tgcgggccat
     3841 tctccagaat cagggaaaca gcggcagcga gactcccggg acctcagagt ccgccacacc
     3901 cgaaagtgat aaaaagtatt ctattggttt agccatcggc actaattccg ttggatgggc
     3961 tgtcataacc gatgaataca aagtaccttc aaagaaattt aaggtgttgg ggaacacaga
     4021 ccgtcattcg attaaaaaga atcttatcgg tgccctccta ttcgatagtg gcgaaacggc
     4081 agaggcgact cgcctgaaac gaaccgctcg gagaaggtat acacgtcgca agaaccgaat
     4141 atgttactta caagaaattt ttagcaatga gatggccaaa gttgacgatt ctttctttca
     4201 ccgtttggaa gagtccttcc ttgtcgaaga ggacaagaaa catgaacggc accccatctt
     4261 tggaaacata gtagatgagg tggcatatca tgaaaagtac ccaacgattt atcacctcag
     4321 aaaaaagcta gttgactcaa ctgataaagc ggacctgagg ttaatctact tggctcttgc
     4381 ccatatgata aagttccgtg ggcactttct cattgagggt gatctaaatc cggacaactc
     4441 ggatgtcgac aaactgttca tccagttagt acaaacctat aatcagttgt ttgaagagaa
     4501 ccctataaat gcaagtggcg tggatgcgaa ggctattctt agcgcccgcc tctctaaatc
     4561 ccgacggcta gaaaacctga tcgcacaatt acccggagag aagaaaaatg ggttgttcgg
     4621 taaccttata gcgctctcac taggcctgac accaaatttt aagtcgaact tcgacttagc
     4681 tgaagatgcc aaattgcagc ttagtaagga cacgtacgat gacgatctcg acaatctact
     4741 ggcacaaatt ggagatcagt atgcggactt atttttggct gccaaaaacc ttagcgatgc
     4801 aatcctccta tctgacatac tgagagttaa tactgagatt accaaggcgc cgttatccgc
     4861 ttcaatgatc aaaaggtacg atgaacatca ccaagacttg acacttctca aggccctagt
     4921 ccgtcagcaa ctgcctgaga aatataagga aatattcttt gatcagtcga aaaacgggta
     4981 cgcaggttat attgacggcg gagcgagtca agaggaattc tacaagttta tcaaacccat
     5041 attagagaag atggatggga cggaagagtt gcttgtaaaa ctcaatcgcg aagatctact
     5101 gcgaaagcag cggactttcg acaacggtag cattccacat caaatccact taggcgaatt
     5161 gcatgctata cttagaaggc aggaggattt ttatccgttc ctcaaagaca atcgtgaaaa
     5221 gattgagaaa atcctaacct ttcgcatacc ttactatgtg ggacccctgg cccgagggaa
     5281 ctctcggttc gcatggatga caagaaagtc cgaagaaacg attactccat ggaattttga
     5341 ggaagttgtc gataaaggtg cgtcagctca atcgttcatc gagaggatga ccaactttga
     5401 caagaattta ccgaacgaaa aagtattgcc taagcacagt ttactttacg agtatttcac
     5461 agtgtacaat gaactcacga aagttaagta tgtcactgag ggcatgcgta aacccgcctt
     5521 tctaagcgga gaacagaaga aagcaatagt agatctgtta ttcaagacca accgcaaagt
     5581 gacagttaag caattgaaag aggactactt taagaaaatt gaatgcttcg attctgtcga
     5641 gatctccggg gtagaagatc gatttaatgc gtcacttggt acgtatcatg acctcctaaa
     5701 gataattaaa gataaggact tcctggataa cgaagagaat gaagatatct tagaagatat
     5761 agtgttgact cttaccctct ttgaagatcg ggaaatgatt gaggaaagac taaaaacata
     5821 cgctcacctg ttcgacgata aggttatgaa acagttaaag aggcgtcgct atacgggctg
     5881 gggacgattg tcgcggaaac ttatcaacgg gataagagac aagcaaagtg gtaaaactat
     5941 tctcgatttt ctaaagagcg acggcttcgc caataggaac tttatgcagc tgatccatga
     6001 tgactcttta accttcaaag aggatataca aaaggcacag gtttccggac aaggggactc
     6061 attgcacgaa catattgcga atcttgctgg ttcgccagcc atcaaaaagg gcatactcca
     6121 gacagtcaaa gtagtggatg agctagttaa ggtcatggga cgtcacaaac cggaaaacat
     6181 tgtaatcgag atggcacgcg aaaatcaaac gactcagaag gggcaaaaaa acagtcgaga
     6241 gcggatgaag agaatagaag agggtattaa agaactgggc agccagatct taaaggagca
     6301 tcctgtggaa aatacccaat tgcagaacga gaaactttac ctctattacc tacaaaatgg
     6361 aagggacatg tatgttgatc aggaactgga cataaaccgt ttatctgatt acgacgtcga
     6421 tcacattgta ccccaatcct ttttgaagga cgattcaatc gacaataaag tgcttacacg
     6481 ctcggataag aaccgaggga aaagtgacaa tgttccaagc gaggaagtcg taaagaaaat
     6541 gaagaactat tggcggcagc tcctaaatgc gaaactgata acgcaaagaa agttcgataa
     6601 cttaactaaa gctgagaggg gtggcttgtc tgaacttgac aaggccggat ttattaaacg
     6661 tcagctcgtg gaaacccgcc aaatcacaaa gcatgttgca cagatactag attcccgaat
     6721 gaatacgaaa tacgacgaga acgataagct gattcgggaa gtcaaagtaa tcactttaaa
     6781 gtcaaaattg gtgtcggact tcagaaagga ttttcaattc tataaagtta gggagataaa
     6841 taactaccac catgcgcacg acgcttatct taatgccgtc gtagggaccg cactcattaa
     6901 gaaatacccg aagctagaaa gtgagtttgt gtatggtgat tacaaagttt atgacgtccg
     6961 taagatgatc gcgaaaagcg aacaggagat aggcaaggct acagccaaat acttctttta
     7021 ttctaacatt atgaatttct ttaagacgga aatcactctg gcaaacggag agatacgcaa
     7081 acgaccttta attgaaacca atggggagac aggtgaaatc gtatgggata agggccggga
     7141 cttcgcgacg gtgagaaaag ttttgtccat gccccaagtc aacatagtaa agaaaactga
     7201 ggtgcagacc ggagggtttt caaaggaatc gattcttcca aaaaggaata gtgataagct
     7261 catcgctcgt aaaaaggact gggacccgaa aaagtacggt ggcttcgata gccctacagt
     7321 tgcctattct gtcctagtag tggcaaaagt tgagaaggga aaatccaaga aactgaagtc
     7381 agtcaaagaa ttattgggga taacgattat ggagcgctcg tcttttgaaa agaaccccat
     7441 cgacttcctt gaggcgaaag gttacaagga agtaaaaaag gatctcataa ttaaactacc
     7501 aaagtatagt ctgtttgagt tagaaaatgg ccgaaaacgg atgttggcta gcgccggaga
     7561 gcttcaaaag gggaacgaac tcgcactacc gtctaaatac gtgaatttcc tgtatttagc
     7621 gtcccattac gagaagttga aaggttcacc tgaagataac gaacagaagc aactttttgt
     7681 tgagcagcac aaacattatc tcgacgaaat catagagcaa atttcggaat tcagtaagag
     7741 agtcatccta gctgatgcca atctggacaa agtattaagc gcatacaaca agcacaggga
     7801 taaacccata cgtgagcagg cggaaaatat tatccatttg tttactctta ccaacctcgg
     7861 cgctccagcc gcattcaagt attttgacac aacgatagat cgcaaacgat acacttctac
     7921 caaggaggtg ctagacgcga cactgattca ccaatccatc acgggattat atgaaactcg
     7981 gatagatttg tcacagcttg ggggtgactc tggtggttct actaatctgt cagatattat
     8041 tgaaaaggag accggtaagc aactggttat ccaggaatcc atcctcatgc tcccagagga
     8101 ggtggaagaa gtcattggga acaagccgga aagcgatata ctcgtgcaca ccgcctacga
     8161 cgagagcacc gacgagaatg tcatgcttct gactagcgac gcccctgaat acaagccttg
     8221 ggctctggtc atacaggata gcaacggtga gaacaagatt aagatgctct ctggtggttc
     8281 tcccaagaag aagaggaaag tcggaggcgg tggaagcggc gcaacaaact tctctctgct
     8341 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gtgagcaagg gcgaggagct
     8401 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     8461 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     8521 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     8581 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8641 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     8701 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     8761 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     8821 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     8881 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     8941 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     9001 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     9061 cgggatcact ctcggcatgg acgagctgta caagtgaagc ggccgcactc ctcaggtgca
     9121 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     9181 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     9241 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     9301 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     9361 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     9421 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     9481 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     9541 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     9601 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     9661 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9721 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9781 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     9841 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9901 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9961 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10021 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10081 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10141 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
    10201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10681 ttcagcccga ccgctgcgcc ttatmmgstr rsywkygtsy wsrrwccmmc
//
