LOCUS       sequence-254674-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-128-2-5p target in 3' UTR of of mKate. miRNA activity can be
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-128-2-5p
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(117..141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(369..388)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    434..489
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(488..507)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tggaagaatc aaggaggccg acaaagagac atacgtcgag cagcacgagg tggctgtggc
       61 cagatactgc gacctcccta gcaaactggg gcacaaactt aattgagaat tctaccaccc
      121 agctttcttg tacaaagtgg tacgtctcaa gaccagaggt ctctgcttag aggtattctc
      181 gtacagtgta tcggccccct ctcgtacagt gtatcggccc cctctcgtac agtgtatcgg
      241 ccccctctcg tacagtgtat cggcccccca accagaagag acctgtgctt cactcctcag
      301 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
      361 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
      421 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
      481 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
      541 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
      601 taaagaggtc atcagtatat gaaacagccc cctgctgtcc att
//
