LOCUS       sequence-25468-e         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC90
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..39)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(61..81)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(103..123)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(145..165)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    204..251
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(458..474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(458..474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(471..493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    482..498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(506..512,513..530,531..536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    551..572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 actcattttt tctaccggta ccttacgctt cttctttgga gcagtcattt tttctgccgg
       61 taccttacgc ttcttctttg gagcagtcat tttttctgcc ggtaccttac gcttcttctt
      121 tggagcagtc attttttctg ccggtacctt acgcttcttc tttggagcag tcattttttc
      181 tgagctcggt acccggggat cttgaagttc ctattccgaa gttcctattc tctagaaagt
      241 ataggaactt cagagcgctt ttgaagctag aactagtgga tccgcgccaa aaccgcggtc
      301 tagagtcgac ctgcaggcat gcaagcttat ttcatttcca agttgttagc gtatccatcg
      361 ttgtgagtga tttcagccat ttttaagctc tgaaaattta aatgagaata gttagttagt
      421 aagatcatgt ttaaacttac agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      481 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      541 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg ccccgctttc
      601 cagtcc
//
