LOCUS       sequence-25470-e         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC292
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..48)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(70..90)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(154..174)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    251..298
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(505..521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(518..540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    529..545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(553..559,560..577,578..583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttctccttta ctcatttttt ctaccggtac cttacgcttc ttctttggag cagtcatttt
       61 ttctgccggt accttacgct tcttctttgg agcagtcatt ttttctgccg gtaccttacg
      121 cttcttcttt ggagcagtca ttttttctgc cggtacctta cgcttcttct ttggagcagt
      181 cattttttct gagctcctga aaatgttcta tgttatgtta gtatcattcg aaacataccc
      241 cggggatctt gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag
      301 agcgcttttg aagctagaac tagtggatcc gcgccaaaac cgcggtctag agtcgacctg
      361 caggcatgca agcttatttc atttccaagt tgttagcgta tccatcgttg tgagtgattt
      421 cagccatttt taagctctga aaatttaaat gagaatagtt agttagtaag atcatgttta
      481 aacttacagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgccaa
//
