LOCUS       sequence-254700-        6300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  4323 b scaffold from compatibility group 4, made by method IV, 
            insert based on de Bruijn sequence with the an order of 7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_IV_CS6_4323_v1
FEATURES             Location/Qualifiers
     source          1..6300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(337..925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(417..436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1108..1202)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1223..1882)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1797..1816
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2064..2082
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttgagaccgg gagcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
       61 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ccgggatcgg aattccggcc
      121 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
      181 actcttgttc caaactggaa aacgtctagc ggtatcagct cactcaaagg cggtaatacg
      241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
      361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1081 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     1141 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     1201 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1261 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1321 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1381 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1441 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1501 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     1561 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     1621 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     1681 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     1741 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     1801 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     1861 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     1921 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     1981 cgatcaaggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2041 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agcctcccca
     2101 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2161 ctcttgttcc aaactggaac aacactcaac cctatctcgg gcaagcttgt agtctaacgg
     2221 caggtcagca ggcccgaatc ttcggattgt tgctgcagca ctctataagg ctgactagtc
     2281 cgccaagagt tttttaaaac atcagtggta cttatggaac gacaaacggg tagtgatcca
     2341 cctttttggt aatatcccct gcgggaagct atgggtgggc ggccccgtgc gtgcatgcag
     2401 tcggaccttc cctttgcgct tagccgtccc gcgtttcggc gaagagctcc ttcgtatggt
     2461 atattcccgt tcccaaaacg atcgggatgc ggagacgctc agccaggaat agcttcgcta
     2521 catctcgacc ctggctgccc ccatccgtag aagccggcac ctagctgcta aactaactcg
     2581 gtaaccccaa taacgaactg attgagtgcc atcgtgtaag cggtgcgagt cacgcctggt
     2641 ttcagtaatt agaccaggga ttccttaagt tatctcctgg gactgaagaa gtgagggaaa
     2701 gaacggacat cgagttgtga tatacgtcca acttccggcg tacgacggta ttctgccttc
     2761 tacgagtggc cttagtcgaa caaattacat tagctcaacg gtcgctcgtc ctgccagtat
     2821 actgacccgt accttacgat atcttgacca aacccttgca cggtttggca gtgcaattca
     2881 ctaggggcgc tagcccgtct atgacttacc cctagtgttt agcatacgaa ggaagtttct
     2941 tcttattcaa cactgagtta gtagcgggtt agcgaggatt actgttcagc tgaactccgt
     3001 gggatatggc gggcgataac tttcaagatc gccctgtttc ctgtgtcggt ccaggttgac
     3061 atactccatc taatcagcgt aggatgatct gtggggaacc tacactactt gcggttgttt
     3121 tatccggtct agactcacag tcctcgttgt cgtacatgat ggcccaacat gccctacggc
     3181 gccttgtgca caggtacagg aggcaagtca ggcggtcaag gaccataaag cgatctatcg
     3241 acatttatac agagcagaat gcgtaaaggg cttgagcatg tcgccacagc gacacttctc
     3301 gccgtgatgt tactttaggt tataaccacg actgcatcca aggtgccgcc ggtacggaag
     3361 gcactatcta gcgcaactgt ataaaagatg caccgttaga acagccgcgc gtccggaggt
     3421 ttatgtatgt gaacacaacg cacccactcg agctgttggt tccgtcgtta cgccgagtaa
     3481 gacgtatagg cgagacaggc atagcctaaa gaggagtacc acaaaaatca tatgttctcc
     3541 aaatcccaga tgtctttcca ttctattagg gaccggatgg gccggacgac cgctggggca
     3601 aaaggtccga cctaatgata ggtgtccccg acagaagggt tgcgttaatg gtcgtgactg
     3661 gacccatgcg atgcttttcg aggggttttg ctagatgaaa ctctggtgat taagggggtc
     3721 atcttttacg taatctacct ggatgtaaat tttagatctc tcatcacgag gtcgagaaga
     3781 caactacaaa gactgtcaat tgatttgtgt atcgtaagtg gagggcgtgt catgtagtct
     3841 gtctgacggc ctgtcgactt tgggctgtag ccacttttga tgagtccctc aaaatgccaa
     3901 tgtttgcaag cgcgagcaag acccccgggg ggatcgtcgc aaactggtag cagttaccaa
     3961 ccgtatccat ggtgcagaca tgggggctcc agaggccatg acgcataagc aaaggatagt
     4021 ttgtcagaac ccgaggcgcg gacttgtacc gaaccggtga gtagtaaaca acctgacagc
     4081 taatttaaga gaaatcgatc attacgggga cagtagggtg aagcatcggt gtaggctatt
     4141 gtattatcgg caacgagagc cccttcatcg cacttagagg gtatcaggac gcggctcgct
     4201 tcgcgtggtc ccatacatag gactatgtcc taagattgcg actaccatca tgaggtgacg
     4261 aattgtccgt ttaacgcttg tctactgggc agatcaagct ttactaatac ctcaccctcg
     4321 gggtaaaaaa cctccgatcc tgatcagata atgtacaccc ggaacataac agtgtacgct
     4381 aacaatttct agttgcattt tgtagacggg ctaagtccac gcaagggagt cgcgattagt
     4441 gctcgctgtc catatatcaa acagactaaa attggggtgc tgtgcttata taatccagcg
     4501 ccgcatggac ggataaacgt actcgcgggg ccaaagtgtc tctgttaaac cagtttacct
     4561 atatgctata gtatctgcac tggccagaaa ataattgcca cgtctcacgt gtgcccgccc
     4621 agtgacctgc tgagcctgag gcttaaagtt caaagcagcg gcattcaggg catcaaccca
     4681 agcacacacc tgtaactaga gtcttcaatg ggaattccgc gaatggcttc caatcactgt
     4741 gagagtatga gcggaaacac gttttcaccg cacaagtgcg cgctcccccc tccacattga
     4801 ctccctgaaa acttgataag tagatatttc ataataagaa agcccacacg cgaccacccc
     4861 gctctaagga gctaccctaa ccgagataga cacgaaaggc gttcgacgtg aatcggagca
     4921 ccagcaacag ggtcggccga tgacacatcc ctatgcctcg attcatttgc ctactatttt
     4981 ccccacggct ggtcttggaa gatactagca cgatggttgg gttctttgag gaaaagctct
     5041 tgttatgcaa ccatttccga gcgctgcgcc ccaggctctg cttgccgggc acatgtgcgg
     5101 cggatcttac aacaaggccg ctatcatcct tggctcatag agagattttt ctgggtaccc
     5161 gcagccctct acagttcgcc tattcggttc atggcatgaa ggtaaggttc gggtgttagg
     5221 agaggtagag cgttggcgtc atacccagcc tttctcttta atcgcttcag agacctcgca
     5281 ttgtggactc tctaggtctg gagcgaacgc gtatttacac ggagttctaa atgaataaat
     5341 atgattcttg cttctgtact gccgacgatt ggccgtaaca ttcgatacgc agagtgtgtg
     5401 gcgacgcccg gctactctta ggcctcaggt ggtgtgacaa gccaacgtgc ctgcaaatgt
     5461 gttgatcgaa gtattggatc acctcttcct cccaccgggt ctcggaatca atcgttcacg
     5521 ggaggacacc gatttagttc ctatcctaga aaccgcctcc tctcgtgcta cgcgcagtac
     5581 gttctgagaa ctataccgcg gtaggtatgc gcatctggca atagtgggtc ctttattgaa
     5641 atggatttcg tggcacgctg atgccgttgc ccattggtgg ataccagacc gtgttcgtca
     5701 actcaatacg gtggctagga acttaatagg gcccttatca cactcattcc agtctccgct
     5761 ttgttccact gcgtcttaac accactaagc tggaatgtca caatattacc ggccaccaag
     5821 ttggacaatg agatggagaa tttggagtga aagtcgtctg cgaaacgcca ttattaattc
     5881 gtttgacgtt atttgaacca attatgaacg tcaccatgtt gtaatgcata tcgcgccagc
     5941 tcgaaataca cagattcgca ggggagccat agtcaaatag aatatagatt atagcaatgc
     6001 tcactcctac cgtcagtcat tgctctcccg atagcgtcga tgtggttaag cctctgatac
     6061 aatctcaagt actacgtgga aattctcagt tgagacttcg aatacttcac atattgcagg
     6121 atccgcaatc ctcatgctgg cgcacgtagt taaccttgaa ttaactgctc cgggacgtcg
     6181 ggcctaggca gcttactcag acgagccgaa gcgtgagctt ggtcacttgg gagatcccgg
     6241 ttacagcatt aaatctgaat gaccgactcg tagctagtac aagaatccga aaaagtaacg
//
