LOCUS       sequence-254706-       10039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible N2H assay destination vector with N-terminus 
            tagging of nanoluc fragment1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, 
            Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan 
            S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker 
            EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal 
            M, Calderwood MA, Jacob Y
  TITLE     Maximizing binary interactome mapping with a minimal number of 
            assays.
  JOURNAL   Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2.
  PUBMED    31467278
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-29"; volume: "10(1):3907. doi"; pages: " 
            10.1038/s41467-019-11809-2"
COMMENT     Sequence Label: pDEST-N2H-N1
FEATURES             Location/Qualifiers
     source          1..10039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        177..584
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             585..1679
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(2173..2192)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2292..2314
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2352..2370)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2411..3753
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    2743..2790
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        3780..3884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3885..3953,3954..4745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4103..4122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4916..5504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5405..5424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5658..5675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5774..5909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    5924..5957
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     terminator      complement(5962..6149)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     6070..6094
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    6214..6335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6251..6270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6305..6324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6346..6379
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6417..6541
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6582..6887)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6684..6703)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(7229..7888)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7803..7822
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(7942..7948,7949..7966,7967..7972))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(7997..8121)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8548..8567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8549..8567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        8944..9323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      9460..9915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9547..9566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9757..9778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acaattacat caaaatccac attctcttca aaatcaattg tcctgtactt ccttgttcat
       61 gtgtgttcaa aaacgttata tttataggat aattatactc tatttctcaa caagtaattg
      121 gttgtttggc cgagcggtct aaggcgcctg attcaagaaa tatcttgacc gcagttaact
      181 gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt
      241 tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
      301 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
      361 tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg
      421 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat
      481 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca
      541 tttcagcaat atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga
      601 agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg
      661 ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa
      721 ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg ctggaagcct
      781 ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta
      841 gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact
      901 taagaccatg taactttgca tccgactctc ttttagactt atctccaatc aagccacaat
      961 ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta
     1021 agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag
     1081 aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc
     1141 ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg
     1201 tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa ttgattgatt
     1261 ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca
     1321 acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt
     1381 tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac
     1441 catgccacgg ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct
     1501 tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg
     1561 aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca
     1621 acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa
     1681 aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa
     1741 acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc
     1801 tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt
     1861 gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa
     1921 ggaggagggc accacacaaa aagttaggtg taacagaaaa tcatgaaact atgattccta
     1981 atttatatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa aaaacactca
     2041 atgacctgac catttgatgg agtttaagtc aataccttct tgaaccattt cccataatgg
     2101 tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttaacgacgt agtcgatatg
     2161 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2221 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2281 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2341 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2401 ttcttagtat gatccaatat caaaggaaat gatagcattg aaggatgaga ctaatccaat
     2461 tgaggagtgg cagcatatag aacagctaaa gggtagtgct gaaggaagca tacgataccc
     2521 cgcatggaat gggataatat cacaggaggt actagactac ctttcatcct acataaatag
     2581 acgcatataa gtacgcattt aagcataaac acgcactatg ccgttcttct catgtatata
     2641 tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc
     2701 tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag
     2761 ttcctattct ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg
     2821 aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc
     2881 gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat
     2941 atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct
     3001 ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac
     3061 tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat
     3121 agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta
     3181 tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa tcggggatgc
     3241 ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc gggaagtgga
     3301 gtcaggcttt ttttatggaa gagaaaatag acaccaaagt agccttcttc taaccttaac
     3361 ggacctacag tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa aggagaaaaa
     3421 aagtaatcta agatgctttg ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca
     3481 aaaaagaagt atagattctt tgttggtaaa atagcgctct cgcgttgcat ttctgttctg
     3541 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
     3601 ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg
     3661 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt
     3721 ttgttctaca aaatgaagca cagatgcttc gttcaggtgg cacttttcgg ggaaatgtgc
     3781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt atcattgcag
     4621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5701 aaccgcctct ccccgcgcgt tggccgattc attaatgcag tcctgtctct tgttcgatct
     5761 ttgcaaaagc ctcggcctcc aaaaaagcgt cctcactact tctggaatag ctcagaggcc
     5821 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgagg cggagaatgg
     5881 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggcataactt cgtataaggt
     5941 atactatacg aagttattcc gccggtagag gtgtggtcaa taagagcgac ctcatgctat
     6001 acctgagaaa gcaacctgac ctacaggaaa gagttactca agaacaagaa ttttcgtttt
     6061 aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac ttatttaata
     6121 ataaaaatca taaatcataa gaaattcgct ttaaaaaacc tcccacatct ccccctgaac
     6181 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6241 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6301 tgttgtggtt tgtccaaact catcaatgta tcttatcatg tctccataac ttcgtataat
     6361 gtatgctata cgaagttatg ttcgctacct taggaccgtt atagttactt acttacacca
     6421 ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     6481 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     6541 ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
     6601 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
     6661 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
     6721 atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc
     6781 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
     6841 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
     6901 gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
     6961 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
     7021 gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
     7081 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat
     7141 acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc
     7201 tggcttttag taagccggat ccacgcgttt acgccccgcc ctgccactca tcgcagtact
     7261 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     7321 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     7381 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     7441 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     7501 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     7561 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     7621 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     7681 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     7741 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     7801 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     7861 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg
     7921 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
     7981 tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     8041 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     8101 agcttttttg tacaaacttg tgatgggact gccccctcca ccactgcccc ctccaccttc
     8161 atacgggatg atgacatgga tgtcgatctt cagcccattt tcaccgctca ggacaatcct
     8221 ttggatcgga gttacggaca ccccgagatt ctgaaacaaa ctggacacac ctccctgttc
     8281 aaggacttgg tccaggttgt agccggctgt ctgtcgccag tccccaacga aatcttcgag
     8341 tgtgaagacg cccataggta aatttcttag gtttttcgac tacctaatct gaaaaaccgc
     8401 agagagggct tcctggatat tgtggggctg ccactaggca tcctggaacg taaagctaaa
     8461 tagctaacct aaatacaaaa atagcactac ttgtaattta ttaacctctc aaaattgtat
     8521 ttacaagcaa gatcacattt ttgctttccc tatagtgagt cgtattaaga gctcggtgtt
     8581 cgaggatcca cacgcgtcac cttttatatg cgaagtggac ctgggaccgc gccgccccga
     8641 ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat
     8701 agatctaaag acatgcttaa ttaatcacat gctaggcgcg ccttaattaa aaatttcagt
     8761 ggcgcgcctt aattaattca aggaggggcg cgcccgatcg cagttgttac gacattttgg
     8821 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     8881 tggaaatccc cgtgagtcaa accgctatcc acgcccattg gtgtactgcc aaaaccgcat
     8941 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccgtaa
     9001 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     9061 cggacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     9121 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     9181 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     9241 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     9301 ataactagtc aataatcaat gccaacatgg cggtcatatt ggacatgagc caatataaat
     9361 gtacatatta tgatatagat acaacgtatg caatggccaa tagccaatat tgatttatgc
     9421 tatataacca atgaataata tggctaatgg ccaatattga cgcgccctgt agcggcgcat
     9481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9661 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9781 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     9901 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     9961 ccgcatatcg accggtcgag gagaacttct agtatatcta catacctaat attattgcct
    10021 tattaaaaat ggaatccca
//
