LOCUS       sequence-25471-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC305
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..49)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    126..173
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(380..396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(380..396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(393..415)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    404..420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(428..434,435..452,453..458))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    473..494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(611..628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttctccttt actcattttt tctaccggta ccttacgctt cttctttgga gcagtcattt
       61 tttctgagct cctgaaaatg ttctatgtta tgttagtatc attcgaaaca taccccgggg
      121 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
      181 ttttgaagct agaactagtg gatccgcgcc aaaaccgcgg tctagagtcg acctgcaggc
      241 atgcaagctt atttcatttc caagttgtta gcgtatccat cgttgtgagt gatttcagcc
      301 atttttaagc tctgaaaatt taaatgagaa tagttagtta gtaagatcat gtttaaactt
      361 acagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      421 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      481 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      541 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      661 cagctcactc aaaggcggta atacggtttt
//
