LOCUS       sequence-25473-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC80
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(180..227)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(389..407)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(439..459)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 aactgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
       61 tcactcacaa cgatggatac gctaacaact tggaaatgaa ataagcttgc atgcctgcag
      121 gtcgactcta gaccgcggtt ttggcgcgga tccactagtt ctagcttcaa aagcgctctg
      181 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaag atccccggta
      241 gacgataagg atccgatggt ggcctcctcc gaggacgtca tcaaagagtt catgcgcttc
      301 aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      361 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      421 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc gtacgtgaag
      481 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
      541 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccagg
//
