LOCUS       sequence-254732-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-1285-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-1285-3p
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(97..121)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(345..364)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    410..465
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(464..483)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 acaaagagac atacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
       61 gcaaactggg gcacaaactt aattgagaat tctaccaccc agctttcttg tacaaagtgg
      121 tacgtctcaa gaccagaggt ctctgcttga actcttacgt ctcactttgt tgcccagaac
      181 gtctcacttt gttgcccaga acgtctcact ttgttgccca gaacgtctca ctttgttgcc
      241 cagacaacca gaagagacct gtgcttcact cctcaggtgc aggctgccta tcagaaggtg
      301 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
      361 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      421 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      481 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
      541 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
      601 cagccccctg ctgtccattc cttattc
//
