LOCUS       sequence-254737-        4035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TtNAM-B1 CDS in the Gateway-compatible pCR8 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pCR8_TtNAM-B1
FEATURES             Location/Qualifiers
     source          1..4035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1346..1445)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1462..1481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1463..1481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1486..1502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1486..1502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1933..2724
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2817..3405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3306..3325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3559..3576
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3735..3762)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3854..3940)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3989..4011
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4003..4020
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4004..4020
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttatgggc
      121 agctccgact catcttccgg ctcggcgcaa aaagcaacgc ggtatcacca tcagcatcag
      181 ccgccgcctc cgcagcgggg ctcggcgccg gagctcccgc cgggcttccg gttccacccg
      241 acggacgagg agctggtggt gcactacctc aagaagaagg ccgacaaggc gccgctcccc
      301 gtcaacatca tcgccgaggt ggatctctac aagttcgacc catgggagct ccccgagaag
      361 gcgaccatcg gggagcagga gtggtacttc ttcagcccgc gcgaccgcaa gtaccccaac
      421 ggcgcgcggc cgaaccgggc ggcgacgtcg gggtactgga aggccaccgg cacggacaag
      481 cctatcctgg cctcggggac ggggtgcggc ctggtccggg agaagctcgg cgtcaagaag
      541 gcgctcgtgt tctaccgcgg gaagccgccc aagggcctca aaaccaactg gatcatgcac
      601 gagtaccgcc tcaccgacgc atctggctcc accaccgcca ccaaccgacc gccgccggtg
      661 accggcggga gcagggctgc tgcctctctc aggttggacg actgggtgct gtgccgcatc
      721 tacaagaaga tcaacaaggc cgcggccggc gatcagcaga ggaacacgga gtgcgaggac
      781 tccgtggagg acgcggtcac cgcgtacccg ctctatgcca cggcgggcat gaccggtgca
      841 ggtgcgcatg gcagcaacta cgcttcacct tcactgctcc atcatcagga cagccatttc
      901 ctggacggcc tgttcacagc agacgacgcc ggcctctcgg cgggcgccac ctcgctgagc
      961 cacctagcag cggcggcgag ggcaagcccg gctccgacca aacagtttct cgccccgtcg
     1021 tcttcgaccc cgttcaactg gctcgatgcg tcaccagtcg gcatcctccc acaggcaagg
     1081 aattttcctg ggtttaacag gagcagaaac gtcggcaata tgtctctgtc atcgacggcc
     1141 gacatggctg gcgcagtgga caacggtgga ggcaatgcgg tgaacgccat gtctacctat
     1201 cttcccgtgc aagacgggac gtaccatcag cagcatgtca tcctcggcgc tccgctggtg
     1261 ccagaagccg ccgccgccac ctctggattc cagcatcccg ttcaaatatc cggcgtgaac
     1321 tggaatccct gaaagggcga attcgaccca gctttcttgt acaaagttgg cattataaaa
     1381 aataattgct catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1441 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1501 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1561 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     1621 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     1681 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     1741 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     1801 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1861 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1921 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1981 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2041 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     2101 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2161 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2221 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2281 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2341 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2401 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     2461 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     2521 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     2581 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2641 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     2701 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     2761 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2821 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2881 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2941 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3001 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3061 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3121 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3181 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3241 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3361 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3421 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3541 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3601 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3661 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     3721 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3781 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3841 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3901 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     3961 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4021 cttaagctcg ggccc
//
