LOCUS       sequence-254738-       16872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length TtNAM-B1 with an N-terminal FLAG tag under the
            35s promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16872)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 16872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pGWB12_TtNAM-B1
FEATURES             Location/Qualifiers
     source          1..16872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1063..1087)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1097..1120)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(1141..1486)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2010..2026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2010..2026)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2023..2045)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2034..2050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2058..2064,2065..2082,2083..2088))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2103..2124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2673..2925)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3315..4109)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3426..3445)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4036..4055
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4130..4313)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4166..4345)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4469..4493
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4958..5608
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(5796..5813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6047..6066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            6289..6398
                     /note="incP origin of transfer"
     CDS             6431..6802
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      6929..7640
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  8263..9030
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             9222..10016
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             10315..11463
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13059..13083
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(13330..13351)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13542..13561
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13686..13708
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13700..13717
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13701..13717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        13859..14203
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             14213..15238
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             14213..15238
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      15706..15958
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(16387..16639)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    16665..16689
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtccactgc gccagccatg tcggccgtcg atgacagaga catattgccg acgtttctgc
       61 tcctgttaaa cccaggaaaa ttccttgcct gtgggaggat gccgactggt gacgcatcga
      121 gccagttgaa cggggtcgaa gacgacgggg cgagaaactg tttggtcgga gccgggcttg
      181 ccctcgccgc cgctgctagg tggctcagcg aggtggcgcc cgccgagagg ccggcgtcgt
      241 ctgctgtgaa caggccgtcc aggaaatggc tgtcctgatg atggagcagt gaaggtgaag
      301 cgtagttgct gccatgcgca cctgcaccgg tcatgcccgc cgtggcatag agcgggtacg
      361 cggtgaccgc gtcctccacg gagtcctcgc actccgtgtt cctctgctga tcgccggccg
      421 cggccttgtt gatcttcttg tagatgcggc acagcaccca gtcgtccaac ctgagagagg
      481 cagcagccct gctcccgccg gtcaccggcg gcggtcggtt ggtggcggtg gtggagccag
      541 atgcgtcggt gaggcggtac tcgtgcatga tccagttggt tttgaggccc ttgggcggct
      601 tcccgcggta gaacacgagc gccttcttga cgccgagctt ctcccggacc aggccgcacc
      661 ccgtccccga ggccaggata ggcttgtccg tgccggtggc cttccagtac cccgacgtcg
      721 ccgcccggtt cggccgcgcg ccgttggggt acttgcggtc gcgcgggctg aagaagtacc
      781 actcctgctc cccgatggtc gccttctcgg ggagctccca tgggtcgaac ttgtagagat
      841 ccacctcggc gatgatgttg acggggagcg gcgccttgtc ggccttcttc ttgaggtagt
      901 gcaccaccag ctcctcgtcc gtcgggtgga accggaagcc cggcgggagc tccggcgccg
      961 agccccgctg cggaggcggc ggctgatgct gatggtgata ccgcgttgct ttttgcgccg
     1021 agccggaaga tgagtcggag ctgcccataa gggcgaattc ggagcctgct tttttgtaca
     1081 aacttgtgat aacagccttg tcatcgtcat ccttgtagtc gctcattcta gagtcccccg
     1141 tgttctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     1201 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     1261 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     1321 accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat gatggcattt
     1381 gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag ctgggcaatg
     1441 gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg ccctttggtc
     1501 ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt
     1561 gacgaagatt ttcttcttgt cattgagtcg taagagactc tgtatgaact gttcgccagt
     1621 ctttacggcg agttctgtta ggtcctctat ttgaatcttt gactccatgg cctttgattc
     1681 agtgggaact acctttttag agactccaat ctctattact tgccttggtt tgtgaagcaa
     1741 gccttgaatc gtccatactg gaatagtact tctgatcttg agaaatatat ctttctctgt
     1801 gttcttgatg cagttagtcc tgaatctttt gactgcatct ttaaccttct tgggaaggta
     1861 tttgatttcc tggagattat tgctcgggta gatcgtcttg atgagacctg ctgcgtaagc
     1921 ctctctaacc atctgtgggt tagcattctt tctgaaattg aaaaggctaa tctggggacc
     1981 tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2041 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2101 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2161 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2221 attgggccaa agacaaaagg gcgacattca accgattgag ggagggaagg taaatattga
     2281 cggaaattat tcattaaagg tgaattatca ccgtcaccga cttgagccat ttgggaatta
     2341 gagccagcaa aatcaccagt agcaccatta ccattagcaa ggccggaaac gtcaccaatg
     2401 aaaccatcga tagcagcacc gtaatcagta gcgacagaat caagtttgcc tttagcgtca
     2461 gactgtagcg cgttttcatc ggcattttcg gtcatagccc ccttattagc gtttgccatc
     2521 ttttcataat caaaatcacc ggaaccagag ccaccaccgg aaccgcctcc ctcagagccg
     2581 ccaccctcag aaccgccacc ctcagagcca ccaccctcag agccgccacc agaaccacca
     2641 ccagagccgc cgccagcatt gacaggaggc ccgatctagt aacatagatg acaccgcgcg
     2701 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
     2761 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
     2821 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
     2881 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga tcatccgggt
     2941 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt
     3001 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga tcatattgtc
     3061 gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc
     3121 gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat ccccgcgctg
     3181 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     3241 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     3301 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     3361 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     3421 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     3481 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     3541 tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag cctggcgaac
     3601 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     3661 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     3721 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     3781 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     3841 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     3901 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     3961 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     4021 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     4081 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt gcagattatt
     4141 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     4201 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     4261 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     4321 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     4381 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcag attgtcgttt
     4441 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     4501 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     4561 gtccatttgt atgtgcatgc caaccacagg gttccccaga tctggcgccg gccagcgaga
     4621 cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg
     4681 caaattgcac caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg
     4741 agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag
     4801 cgtcgagcgc gacagtgctc agaattacga tcaggggtat gttgggtttc acgtctggcc
     4861 tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact gataagttgg
     4921 tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac
     4981 agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg
     5041 caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact tcaggaacaa
     5101 gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt
     5161 gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca gcttcaggca
     5221 ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc
     5281 gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg
     5341 ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg tgcttgagga
     5401 gcaggccggc gacagcgatg ccggcgagcg cggcggcacc gttgaacagg ctccgctctc
     5461 gccgctgttg cgggccgcga tagacgcctt cgacgaagcc ggtccggacg cagcgttcga
     5521 gcagggactc gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt
     5581 tgaaggaccg agaaagggtg acgattgatc aggaccgctg ccggagcgca acccactcac
     5641 tacagcagag ccatgtagac aacatcccct ccccctttcc accgcgtcag acgcccgtag
     5701 cagcccgcta cgggcttttt catgccctgc cctagcgtcc aagcctcacg gccgcgctcg
     5761 gcctctctgg cggccttctg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5821 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5881 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5941 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6001 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6061 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6121 ctgtccgcct ttctcccttc gggaagcgtg gcgcttttcc gctgcataac cctgcttcgg
     6181 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
     6241 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
     6301 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     6361 ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga
     6421 gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt
     6481 gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag
     6541 cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg tcgtggacta
     6601 tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct
     6661 gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc
     6721 cctgctggcg aagatcgaag agaagcagga cgagcttggc aaggtcatga tgggcgtggt
     6781 ccgcccgagg gcagagccat gactttttta gccgctaaaa cggccggggg gtgcgcgtga
     6841 ttgccaagca cgtccccatg cgctccatca agaagagcga cttcgcggag ctggtgaagt
     6901 acatcaccga cgagcaaggc aagaccgagc gcctttccga cgctcaccgg gctggttgcc
     6961 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
     7021 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
     7081 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     7141 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     7201 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     7261 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     7321 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     7381 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
     7441 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
     7501 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
     7561 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
     7621 cctcacccca aaaatggcag cgctggcagt ccttgccatt gccgggatcg gggcagtaac
     7681 gggatgggcg atcagcccga gcgcgacgcc cggaagcatt gacgtgccgc aggtgctggc
     7741 atcgacattc agcgaccagg tgccgggcag tgagggcggc ggcctgggtg gcggcctgcc
     7801 cttcacttcg gccgtcgggg cattcacgga cttcatggcg gggccggcaa tttttacctt
     7861 gggcattctt ggcatagtgg tcgcgggtgc cgtgctcgtg ttcgggggtg cgataaaccc
     7921 agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga gaattggacc
     7981 tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga agaggatgaa
     8041 gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat aatatatctt
     8101 ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag gcagcgcgct
     8161 tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa tgcttgaaac
     8221 ccaggacaat aaccttatag cttgtaaatt ctatcataat tgggtaatga ctccaactta
     8281 ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt
     8341 tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg
     8401 ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga
     8461 ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct
     8521 cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt
     8581 ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc
     8641 ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt
     8701 taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata
     8761 atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc
     8821 gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag
     8881 cagagatagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct
     8941 gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat
     9001 acactaaatc agtaagttgg cagcatcacc cataattgtg gtttcaaaat cggctccgtc
     9061 gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta
     9121 aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg
     9181 ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat
     9241 caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt
     9301 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
     9361 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
     9421 tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca
     9481 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
     9541 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
     9601 tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac
     9661 tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag
     9721 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
     9781 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
     9841 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
     9901 cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
     9961 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc
    10021 tagatgtggc gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag
    10081 tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc
    10141 gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc
    10201 gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag
    10261 gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat
    10321 cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag
    10381 gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc
    10441 gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct
    10501 ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag
    10561 gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag
    10621 cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg
    10681 ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg
    10741 tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg
    10801 cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag
    10861 gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg
    10921 cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc
    10981 tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag
    11041 gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac
    11101 ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc
    11161 cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg
    11221 aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat
    11281 ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga
    11341 tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg
    11401 cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc
    11461 tagggccttg tggggtcagt tccggctggg ggttcagcag ccagcgcttt actggcattt
    11521 caggaacaag cgggcactgc tcgacgcact tgcttcgctc agtatcgctc gggacgcacg
    11581 gcgcgctcta cgaactgccg ataaacagag gattaaaatt gacaattgtg attaaggctc
    11641 agattcgacg gcttggagcg gccgacgtgc aggatttccg cgagatccga ttgtcggccc
    11701 tgaagaaagc tccagagatg ttcgggtccg tttacgagca cgaggagaaa aagcccatgg
    11761 aggcgttcgc tgaacggttg cgagatgccg tggcattcgg cgcctacatc gacggcgaga
    11821 tcattgggct gtcggtcttc aaacaggagg acggccccaa ggacgctcac aaggcgcatc
    11881 tgtccggcgt tttcgtggag cccgaacagc gaggccgagg ggtcgccggt atgctgctgc
    11941 gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg acagattcca acgggaatct
    12001 ggtggatgcg catcttcatc ctcggcgcac ttaatatttc gctattctgg agcttgttgt
    12061 ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct gtgcagccgc
    12121 tgatggtcgt gttcatctct gccgctctgc taggtagccc gatacgattg atggcggtcc
    12181 tgggggctat ttgcggaact gcgggcgtgg cgctgttggt gttgacacca aacgcagcgc
    12241 tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg ttcggaaccg
    12301 tgctgacccg caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc tggcaactgg
    12361 cggccggagg acttctgctc gttccagtag ctttagtgtt tgatccgcca atcccgatgc
    12421 ctacaggaac caatgttctc ggcctggcgt ggctcggcct gatcggagcg ggtttaacct
    12481 acttcctttg gttccggggg atctcgcgac tcgaacctac agttgtttcc ttactgggct
    12541 ttctcagccc cagatctggg gtcgatcagc cggggatgca tcaggccgac agtcggaact
    12601 tcgggtcccc gacctgtacc attcggtgag caatggatag gggagttgat atcgtcaacg
    12661 ttcacttcta aagaaatagc gccactcagc ttcctcagcg gctttatcca gcgatttcct
    12721 attatgtcgg catagttctc aagatcgaca gcctgtcacg gttaagcgag aaatgaataa
    12781 gaaggctgat aattcggatc tctgcgaggg agatgatatt tgatcacagg cagcaacgct
    12841 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
    12901 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
    12961 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
    13021 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
    13081 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt
    13141 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
    13201 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
    13261 ggtggttccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg
    13321 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
    13381 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt
    13441 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
    13501 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
    13561 gcgggcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    13621 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    13681 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttagcttcta ggacgcgtcg
    13741 acgcgtcgac gcgtcgacgc gtcgacgcgt cctagagatc cgtcaacatg gtggagcacg
    13801 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
    13861 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
    13921 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
    13981 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
    14041 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
    14101 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
    14161 acccttcctc tatataagga agttcatttc atttggagag gacgaccccg atatgaaaaa
    14221 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
    14281 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
    14341 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
    14401 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
    14461 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
    14521 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
    14581 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
    14641 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
    14701 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
    14761 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
    14821 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
    14881 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
    14941 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
    15001 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
    15061 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
    15121 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
    15181 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag
    15241 tagatgccga ccgggatcca acacttacgt ttgcaacgtc caagagcaaa tagaccacga
    15301 acgccggaag gttgccgcag cgtgtggatt gcgtctcaat tctctcttgc aggaatgcaa
    15361 tgatgaatat gatactgact atgaaacttt gagggaatac tgcctagcac cgtcacctca
    15421 taacgtgcat catgcatgcc ctgacaacat ggaacatcgc tatttttctg aagaattatg
    15481 ctcgttggag gatgtcgcgg caattgcagc tattgccaaa atcgaaatac ccctcacgca
    15541 tgcattcatc aatattattc atgcggggaa aggcaagatt aatccaactg gcaaatcatc
    15601 cagcgtgatt ggtaacttca gttccagcga cttgattcgt tttggtgcta cccacgtttt
    15661 caataaggac gagatggtgg agtaaagaag gagtgcgtcg aagcagatcg ttcaaacatt
    15721 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
    15781 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
    15841 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
    15901 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga
    15961 tcaaacttcg gtactgtgta atgacgatga gcaatcgaga ggctgactaa caaaaggtat
    16021 gcccaaaaac aacctctcca aactgtttcg aattggaagt ttctgctcat gccgacaggc
    16081 ataacttaga tattcgcggg ctattcccac taattcgtcc tgctggtttg cgccaagata
    16141 aatcagtgca tctccttaca agttcctctg tcttgtgaaa tgaactgctg actgcccccc
    16201 aagaaagcct cctcatctcc cagttggcgg cggctgatac accatcgaaa acccacgtcc
    16261 gaacacttga tacatgtgcc tgagaaatag gcctacgtcc aagagcaagt cctttctgtg
    16321 ctcgtcggaa attcctctcc tgtcagacgg tcgtgcgcat gtcttgcgtt gatgaagcta
    16381 attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
    16441 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
    16501 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
    16561 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
    16621 gccaaatgtt tgaacgatcg gggaaattcg agctctaggt tatcaccact ttgtacaaga
    16681 aagctgggtc gaattcgccc tttcagggat tccagttcac gccggatatt tgaacgggat
    16741 gctggaatcc agaggtggcg gcggcggctt ctggcaccag cggagcgccg aggatgacat
    16801 gctgctgatg gtacgtcccg tcttgcacgg gaagataggt agacatggcg ttcaccgcat
    16861 tgcctccacc gt
//
