LOCUS       sequence-25474-e         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC179
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(176..223)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             262..282
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             304..324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             346..366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             388..408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(563..581)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggtcg
      121 actctagacc gcggttttgg cgcggatcca ctagttctag cttcaaaagc gctctgaagt
      181 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc ccgggtaccg
      241 agctcagaaa aaatgactgc tccaaagaag aagcgtaagg taccggcaga aaaaatgact
      301 gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt
      361 aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggtagacgat
      421 aaggatccga tggtggcctc ctccgaggac gtcatcaaag agttcatgcg cttcaaggtg
      481 cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      541 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
      601 gcctgggaca tcctgtcccc ccagttcca
//
