LOCUS       sequence-254779-        9644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP 
            mediated by P2A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Shao Z, Zhang R, Khodadadi-Jamayran A, Chen B, Crowley MR, Festok 
            MA, Crossman DK, Townes TM, Hu K
  TITLE     The acetyllysine reader BRD3R promotes human nuclear reprogramming 
            and regulates mitosis.
  JOURNAL   Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
  PUBMED    26947130
REFERENCE   2  (bases 1 to 9644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-7"; volume: "7:10869. doi"; pages: " 
            10.1038/ncomms10869"
COMMENT     Sequence Label: pLVH-EF1a-GFP-P2A-KLF4
FEATURES             Location/Qualifiers
     source          1..9644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2409..3347
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3304..3324
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3364..3373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3370..4086
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             4114..4170
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5612..6200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5665..5685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6083..6094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6407..7040
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7169..7185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7169..7185)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7182..7204)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7193..7209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7217..7223,7224..7241,7242..7247))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7262..7283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7400..7417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7571..8159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7651..7670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8330..9121,9122..9190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8953..8972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9191..9295)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9343..9477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9380..9399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9434..9453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2461 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2521 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2581 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2641 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2701 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2761 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2821 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2881 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2941 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3001 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3061 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3121 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3181 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3241 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3301 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacccg
     3361 gtcgccacca tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
     3421 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
     3481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
     3541 tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
     3601 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
     3661 accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc
     3721 gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc
     3781 ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag
     3841 gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg
     3901 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     3961 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     4021 cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     4081 tacaagtccg gactcagatc tcgaggatct ggagcaacaa acttctcact actcaaacaa
     4141 gcaggtgacg tggaggagaa tcccggcccc atggctgtca gcgacgcgct gctcccatct
     4201 ttctccacgt tcgcgtctgg cccggcggga agggagaaga cactgcgtca agcaggtgcc
     4261 ccgaataacc gctggcggga ggagctctcc cacatgaagc gacttccccc agtgcttccc
     4321 ggccgcccct atgacctggc ggcggcgacc gtggccacag acctggagag cggcggagcc
     4381 ggtgcggctt gcggcggtag caacctggcg cccctacctc ggagagagac cgaggagttc
     4441 aacgatctcc tggacctgga ctttattctc tccaattcgc tgacccatcc tccggagtca
     4501 gtggccgcca ccgtgtcctc gtcagcgtca gcctcctctt cgtcgtcgcc gtcgagcagc
     4561 ggccctgcca gcgcgccctc cacctgcagc ttcacctatc cgatccgggc cgggaacgac
     4621 ccgggcgtgg cgccgggcgg cacgggcgga ggcctcctct atggcaggga gtccgctccc
     4681 cctccgacgg ctcccttcaa cctggcggac atcaacgacg tgagcccctc gggcggcttc
     4741 gtggccgagc tcctgcggcc agaattggac ccggtgtaca ttccgccgca gcagccgcag
     4801 ccgccaggtg gcgggctgat gggcaagttc gtgctgaagg cgtcgctgag cgcccctggc
     4861 agcgagtacg gcagcccgtc ggtcatcagc gtcagcaaag gcagccctga cggcagccac
     4921 ccggtggtgg tggcgcccta caacggcggg ccgccgcgca cgtgccccaa gatcaagcag
     4981 gaggcggtct cttcgtgcac ccacttgggc gctggacccc ctctcagcaa tggccaccgg
     5041 ccggctgcac acgacttccc cctggggcgg cagctcccca gcaggactac cccgaccctg
     5101 ggtcttgagg aagtgctgag cagcagggac tgtcaccctg ccctgccgct tcctcccggc
     5161 ttccatcccc acccggggcc caattaccca tccttcctgc ccgatcagat gcagccgcaa
     5221 gtcccgccgc tccattacca agagctcatg ccacccggtt cctgcatgcc agaggagccc
     5281 aagccaaaga ggggaagacg atcgtggccc cggaaaagga ccgccaccca cacttgtgat
     5341 tacgcgggct gcggcaaaac ctacacaaag agttcccatc tcaaggcaca cctgcgaacc
     5401 cacacaggtg agaaacctta ccactgtgac tgggacggct gtggatggaa attcgcccgc
     5461 tcagatgaac tgaccaggca ctaccgtaaa cacacggggc accgcccgtt ccagtgccaa
     5521 aaatgcgacc gagcattttc caggtcggac cacctcgcct tacacatgaa gaggcatttt
     5581 taaggatcca ccggatctag gcgtctggaa caatcaacct ctggattaca aaatttgtga
     5641 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5701 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5761 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5821 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5881 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5941 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6001 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6061 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6121 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6181 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     6241 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     6301 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     6361 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     6421 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6481 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     6541 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     6601 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     6661 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     6721 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     6781 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     6841 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6901 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6961 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7021 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7081 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     7141 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7201 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7261 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7321 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7381 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7441 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7501 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7561 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7621 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7681 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7741 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7801 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7861 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7921 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7981 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8041 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8101 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8161 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8221 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8281 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8341 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8401 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8461 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8521 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8581 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8641 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8701 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8761 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8821 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8881 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8941 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9001 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9061 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9301 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     9361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9481 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9541 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     9601 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
