LOCUS       sequence-25478-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC383
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(177..224)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(386..404)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(436..456)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     654..673
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
       61 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg cctgcaggtc
      121 gactctagac cgcggttttg gcgcggatcc actagttcta gcttcaaaag cgctctgaag
      181 ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaagatc cccggtagac
      241 gataaggatc cgatggtggc ctcctccgag gacgtcatca aagagttcat gcgcttcaag
      301 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      361 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      421 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggcgta cgtgaagcac
      481 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc
      541 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      601 ggcacgctga tctacaaggt gaagttccgc ggcaccaact tcccccccga cggccccgta
      661 atgcagaaga agaccatggg c
//
