LOCUS       sequence-25479-e         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC78
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(134..181)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             298..318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(408..430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
       61 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg caagctagct
      121 tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt
      181 caagatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac
      241 atagaacatt ttcaggagga cccttggagg gtaccgagct cagaaaaaat gactgctcca
      301 aagaagaagc gtaaggtacc ggtgggtgaa gaccagaaac agcacctcga actgagccgc
      361 gatattgccc agcgtttcaa cgcgctgtat ggcgagatcg atcccgtcgt tttacaacgt
      421 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      481 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgaagg
      541 taagtttaaa cagatccata ctaactaact tgttctgac
//
