LOCUS       sequence-25480-e         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC79
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(172..219)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             336..356
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(446..468)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
       61 aacgatggat acgctaacaa cttggaaatg aaataagctt gcatgcctgc aggtcgactc
      121 tagaccgcgg ttttggcgcg gatccactag ttctagcttc aaaagcgctc tgaagttcct
      181 atactttcta gagaatagga acttcggaat aggaacttca agatccccgg gattggccaa
      241 aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc
      301 cttggagggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
      361 tgggtgaaga ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg
      421 cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      481 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      541 aggcccgcac cgatcgccct tcccaacagt tgcgaaggta agtttaaaca gatccatact
      601 aactaa
//
