LOCUS       sequence-25481-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC256
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(161..181)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(203..223)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(245..265)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(287..307)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    383..430
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(637..653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(637..653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttggtccctt cagcttcacg gtgttgtggc cctcgtaggg gcggccctcg ccctcgccct
       61 cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg caccttgaag cgcatgaact
      121 cggtgatgac gttctcggag gaggccatgg tggcgaccgg taccttacgc ttcttctttg
      181 gagcagtcat tttttctgcc ggtaccttac gcttcttctt tggagcagtc attttttctg
      241 ccggtacctt acgcttcttc tttggagcag tcattttttc tgccggtacc ttacgcttct
      301 tctttggagc agtcattttt tctgagctcc tgaaaatgtt ctatgttatg ttagtatcat
      361 tcgaaacata cccggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta
      421 taggaacttc agagcgcttt tgaagctaga actagtggat ccgcgccaaa accgcggtct
      481 agagtcgacc tgcaggcatg caagcttatt tcatttccaa gttgttagcg tatccatcgt
      541 tgtgagtgat ttcagccatt tttaagctct gaaaatttaa atgagaatag ttagttagta
      601 agatcatgtt taaacttaca gcttggcgta atcatggtca tagctgtttc ctgtgtgaa
//
