LOCUS       sequence-25482-e         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC293
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(169..189)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(211..231)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(253..273)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(295..315)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    392..439
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gccgcccttg gtcaccttca gcttcacggt gttgtggccc tcgtaggggc ggccctcgcc
       61 ctcgccctcg atctcgaact cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg
      121 catgaactcg gtgatgacgt tctcggagga ggccatggtg gcgaccggta ccttacgctt
      181 cttctttgga gcagtcattt tttctgccgg taccttacgc ttcttctttg gagcagtcat
      241 tttttctgcc ggtaccttac gcttcttctt tggagcagtc attttttctg ccggtacctt
      301 acgcttcttc tttggagcag tcattttttc tgagctcctg aaaatgttct atgttatgtt
      361 agtatcattc gaaacatacc ccggggatct tgaagttcct attccgaagt tcctattctc
      421 tagaaagtat aggaacttca gagcgctttt gaagctagaa ctagtggatc cgcgccaaaa
      481 ccgcggtcta gagtcgacct gcaggcatgc aagcttattt catttccaag ttgttagcgt
      541 atccatcgtt gtgagtgatt tcagcctttt ttaagctctg aaaatttaaa tgaaaatagt
      601 tagtta
//
