LOCUS       sequence-254824-        9166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmids encoding human BRD3R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Shao Z, Zhang R, Khodadadi-Jamayran A, Chen B, Crowley MR, Festok 
            MA, Crossman DK, Townes TM, Hu K
  TITLE     The acetyllysine reader BRD3R promotes human nuclear reprogramming 
            and regulates mitosis.
  JOURNAL   Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
  PUBMED    26947130
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-7"; volume: "7:10869. doi"; pages: " 
            10.1038/ncomms10869"
COMMENT     Sequence Label: pLVH-EF1A-BRD3R
FEATURES             Location/Qualifiers
     source          1..9166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2409..3347
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3304..3324
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3371..3395
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5067..5091)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5134..5722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5187..5207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5605..5616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5929..6562
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6691..6707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6691..6707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6704..6726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6715..6731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6739..6745,6746..6763,6764..6769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6784..6805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6922..6939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7093..7681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7173..7192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7852..8643,8644..8712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8475..8494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8713..8817)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8865..8999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8902..8921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8956..8975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2461 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2521 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2581 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2641 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2701 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2761 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2821 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2881 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2941 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3001 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3061 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3121 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3181 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3241 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3301 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacccg
     3361 gtcgaattca acaagtttgt acaaaaaagt tggcaccatg tccaccgcca cgacagtcgc
     3421 ccccgcgggg atcccggcga ccccgggccc tgtgaaccca ccccccccgg aggtctccaa
     3481 ccccagcaag cccggccgca agaccaacca gctgcagtac atgcagaatg tggtggtgaa
     3541 gacgctctgg aaacaccagt tcgcctggcc cttctaccag cccgtggacg caatcaaatt
     3601 gaacctgccg gattatcata aaataattaa aaacccaatg gatatgggga ctattaagaa
     3661 gagactagaa aataattatt attggagtgc aagcgaatgt atgcaggact tcaacaccat
     3721 gtttacaaat tgttacattt ataacaagcc cacagatgac atagtgctaa tggcccaagc
     3781 tttagagaaa atttttctac aaaaagtggc ccagatgccc caagaggaag ttgaattatt
     3841 accccctgct ccaaagggca aaggtcggaa gccggctgcg ggagcccaga gcgcaggtac
     3901 acagcaagtg gcggccgtgt cctctgtctc cccagcgacc ccctttcaga gcgtgccccc
     3961 caccgtctcc cagacgcccg tcatcgctgc cacccctgta ccaaccatca ctgcaaacgt
     4021 cacgtcggtc ccagtccccc cagctgccgc cccacctcct cctgccacac ccatcgtccc
     4081 cgtggtccct cctacgccgc ctgtcgtcaa gaaaaagggc gtgaagcgga aagcagacac
     4141 aaccactccc acgacgtcgg ccatcactgc cagccggagt gagtcgcccc cgccgttgtc
     4201 agaccccaag caggccaaag tggtggcccg gcgggagagt ggtggccgcc ccatcaagcc
     4261 tcccaagaag gacctggagg acggcgaggt gccccagcac gcaggcaaga agggcaagct
     4321 gtcggagcac ctgcgctact gcgacagcat cctcagggag atgctatcca agaagcacgc
     4381 ggcctacgcc tggcccttct acaagccagt ggatgccgag gccctggagc tgcacgacta
     4441 ccacgacatc atcaagcacc cgatggacct cagcaccgtg aaaaggaaga tggatggccg
     4501 agagtaccca gacgcacagg gctttgctgc tgatgtccgg ctgatgttct cgaattgcta
     4561 caaatacaat cccccagacc acgaggttgt ggccatggcc cggaagctcc aggacgtgtt
     4621 tgagatgagg tttgccaaga tgccagatga gcccgtggag gcaccggcgc tgcctgcccc
     4681 tgcggccccc atggtgagca agggcgctga gagcagccgt agcagtgagg agagctcttc
     4741 ggactcaggc agctcggact cggaggagga gcgggccacc aggctggcgg agctgcagga
     4801 gcagctgaag gccgtgcacg agcagctggc cgccctgtct caggccccag taaacaaacc
     4861 aaagaagaag aaggagaaga aggagaagga gaagaagaag aaggacaagg agaaggagaa
     4921 ggagaagcac aaagtgaagg ccgaggaaga gaagaaggcc aaggtggctc cgcctgccaa
     4981 gcaggctcag cagaagaagg ctcctgccaa gaaggccaac agcacgacca cggccggcag
     5041 agatcatttc ttgacctgtg gagttttgcc aactttcttg tacaaagtgg tttagtaact
     5101 agttgagatc caccggatct aggcgtctgg aacaatcaac ctctggatta caaaatttgt
     5161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5521 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     5581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5701 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     5761 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     5821 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     5881 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     5941 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     6001 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     6061 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     6121 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     6181 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     6241 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     6301 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     6361 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6541 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6601 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc
     6661 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8821 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
     8881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     9001 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9061 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9121 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
