LOCUS       sequence-254825-        7333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression human PMS2 protein using baculovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Geng H, Du C, Chen S, Salerno V, Manfredi C, Hsieh P
  TITLE     In vitro studies of DNA mismatch repair proteins.
  JOURNAL   Anal Biochem. 2011 Jun 15;413(2):179-84. doi: 
            10.1016/j.ab.2011.02.017. Epub 2011 Feb 15.
  PUBMED    21329650
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2011.02.017"; journalName: "Anal Biochem"; date: 
            "2011-06-15- 15"; volume: "413"; issue: "2"; pages: "179-84"
COMMENT     Sequence Label: pFB1_hPMS2
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1014..1019,1020..1036,1037..1042))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1394..1485
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1410..1427
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4313..4447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4350..4369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4404..4423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4476..4641
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4825..5279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4912..4931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5121..5142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5305..5409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5410..5478,5479..6270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5628..5647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6441..7029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6930..6949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7183..7200
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
      841 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
      901 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
      961 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     1021 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     1081 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     1141 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     1201 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     1261 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     1321 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc
     1381 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     1441 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     1501 taccgtccca ccatcgggcg cggatccatg gagcgagctg agagctcgag tacagaacct
     1561 gctaaggcca tcaaacctat tgatcggaag tcagtccatc agatttgctc tgggcaggtg
     1621 gtactgagtc taagcactgc ggtaaaggag ttagtagaaa acagtctgga tgctggtgcc
     1681 actaatattg atctaaagct taaggactat ggagtggatc ttattgaagt ttcagacaat
     1741 ggatgtgggg tagaagaaga aaacttcgaa ggcttaactc tgaaacatca cacatctaag
     1801 attcaagagt ttgccgacct aactcaggtt gaaacttttg gctttcgggg ggaagctctg
     1861 agctcacttt gtgcactgag cgatgtcacc atttctacct gccacgcatc ggcgaaggtt
     1921 ggaactcgac tgatgtttga tcacaatggg aaaattatcc agaaaacccc ctacccccgc
     1981 cccagaggga ccacagtcag cgtgcagcag ttattttcca cactacctgt gcgccataag
     2041 gaatttcaaa ggaatattaa gaaggagtat gccaaaatgg tccaggtctt acatgcatac
     2101 tgtatcattt cagcaggcat ccgtgtaagt tgcaccaatc agcttggaca aggaaaacga
     2161 cagcctgtgg tatgcacagg tggaagcccc agcataaagg aaaatatcgg ctctgtgttt
     2221 gggcagaagc agttgcaaag cctcattcct tttgttcagc tgccccctag tgactccgtg
     2281 tgtgaagagt acggtttgag ctgttcggat gctctgcata atctttttta catctcaggt
     2341 ttcatttcac aatgcacgca tggagttgga aggagttcaa cagacagaca gtttttcttt
     2401 atcaaccggc ggccttgtga cccagcaaag gtctgcagac tcgtgaatga ggtctaccac
     2461 atgtataatc gacaccagta tccatttgtt gttcttaaca tttctgttga ttcagaatgc
     2521 gttgatatca atgttactcc agataaaagg caaattttgc tacaagagga aaagcttttg
     2581 ttggcagttt taaagacctc tttgatagga atgtttgata gtgatgtcaa caagctaaat
     2641 gtcagtcagc agccactgct ggatgttgaa ggtaacttaa taaaaatgca tgcagcggat
     2701 ttggaaaagc ccatggtaga aaagcaggat caatcccctt cattaaggac tggagaagaa
     2761 aaaaaagacg tgtccatttc cagactgcga gaggcctttt ctcttcgtca cacaacagag
     2821 aacaagcctc acagcccaaa gactccagaa ccaagaagga gccctctagg acagaaaagg
     2881 ggtatgctgt cttctagcac ttcaggtgcc atctctgaca aaggcgtcct gagatctcag
     2941 aaagaggcag tgagttccag tcacggaccc agtgacccta cggacagagc ggaggtggag
     3001 aaggactcgg ggcacggcag cacttccgtg gattctgagg ggttcagcat cccagacacg
     3061 ggcagtcact gcagcagcga gtatgcggcc agctccccag gggacagggg ctcgcaggaa
     3121 catgtggact ctcaggagaa agcgcctgaa actgacgact ctttttcaga tgtggactgc
     3181 cattcaaacc aggaagatac cggatgtaaa tttcgagttt tgcctcagcc aactaatctc
     3241 gcaaccccaa acacaaagcg ttttaaaaaa gaagaaattc tttccagttc tgacatttgt
     3301 caaaagttag taaatactca ggacatgtca gcctctcagg ttgatgtagc tgtgaaaatt
     3361 aataagaaag ttgtgcccct ggacttttct atgagttctt tagctaaacg aataaagcag
     3421 ttacatcatg aagcacagca aagtgaaggg gaacagaatt acaggaagtt tagggcaaag
     3481 atttgtcctg gagaaaatca agcagccgaa gatgaactaa gaaaagagat aagtaaaacg
     3541 atgtttgcag aaatggaaat cattggtcag tttaacctgg gatttataat aaccaaactg
     3601 aatgaggata tcttcatagt ggaccagcat gccacggacg agaagtataa cttcgagatg
     3661 ctgcagcagc acaccgtgct ccaggggcag aggctcatag cacctcagac tctcaactta
     3721 actgctgtta atgaagctgt tctgatagaa aatctggaaa tatttagaaa gaatggcttt
     3781 gattttgtta tcgatgaaaa tgctccagtc actgaaaggg ctaaactgat ttccttgcca
     3841 actagtaaaa actggacctt cggaccccag gacgtcgatg aactgatctt catgctgagc
     3901 gacagccctg gggtcatgtg ccggccttcc cgagtcaagc agatgtttgc ctccagagcc
     3961 tgccggaagt cggtgatgat tgggactgct ctcaacacaa gcgagatgaa gaaactgatc
     4021 acccacatgg gggagatgga ccacccctgg aactgtcccc atggaaggcc aaccatgaga
     4081 cacatcgcca acctgggtgt catttctcag aactgagtcg acgagctcac tagtcgcggc
     4141 cgctttcgaa tctagagcct gcagtctcga ggcatgcggt accaagcttg tcgagaagta
     4201 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     4261 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     4321 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4381 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4441 ctggatctga tcactgcttg agcctaggag atccgaacca gataagtgaa atctagttcc
     4501 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc
     4561 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc
     4621 caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca
     4681 tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg tcacagaatg
     4741 aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata tcaacgctta
     4801 tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4861 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4921 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4981 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5041 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5101 tggagtccac gttcttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5161 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5221 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     5281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5641 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5941 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7201 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     7261 cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatgcc
     7321 ctgcgtaagc ggg
//
