LOCUS       sequence-254830-       10379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry 
            tagged Seipin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Li S, Prasanna X, Salo VT, Vattulainen I, Ikonen E
  TITLE     An efficient auxin-inducible degron system with low basal 
            degradation in human cells.
  JOURNAL   Nat Methods. 2019 Aug 26. pii: 10.1038/s41592-019-0512-x. doi: 
            10.1038/s41592-019-0512-x.
  PUBMED    31451765
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Aug 26. pii: 10.1038/s41592-019-0512-x. doi: 
            10.1038/s41592-019-0512-x."
COMMENT     Sequence Label: pSH-EFIRES-P-Seipin-miniIAA7-mCherry
FEATURES             Location/Qualifiers
     source          1..10379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(135..152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(306..894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(386..405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1065..1856,1857..1925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1688..1707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1926..2030)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2057..2512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2144..2163)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2354..2375
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    3029..3832
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    3858..3906
                     /note="synthetic polyadenylation signal"
     misc_feature    3920..4011
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3960..3979
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        4023..5185
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4254..5176
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5133..5153
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     intron          5224..5356
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5339..5358
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5401..5419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6894..7601
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7036..7054)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7304..7323
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7620..8186
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7636..8188
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7780..7797)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8007..8026
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8188..8790
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8687..8707
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9024..9105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9061..9080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    9133..9969
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
       61 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
      121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2041 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2101 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2401 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2461 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg
     2521 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2581 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
     2641 gtcgacctac tctcttccgc attggagtcg ctttaactgg ccctggcttt ggcagcctgt
     2701 gctgacccat gcagtcctcc ttaccatccc tccctcgact tcccctcttc cgatgttgag
     2761 cccctccagc cggtcctgga ctttgtctcc ttccctgccc tgccctctcc tgaacctgag
     2821 ccagctccca tagctcagtc tggtctatct gcctggccct ggccattgtc actttgcgct
     2881 gccctcctct cgcccccgag tgcccttgct gtgccgccgg aactctgccc tctaacgctg
     2941 ccgtctctct cctgagtccg gaccactttg agctctactg gcttctgcgc cgcctctggc
     3001 ccactgtttc cccttcccag gcaggtcctg ctttctctga cctgcattct ctcccctggg
     3061 cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct ggcccagatc
     3121 cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc ttgctctctg
     3181 ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc tgcaccacgt
     3241 gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct ctcctccctc
     3301 acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt ctggggcctg
     3361 tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg cgtcccgcct
     3421 ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag taccccgtct
     3481 ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc ccgttctcct
     3541 gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc cccttacctc
     3601 tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg tccgagagct
     3661 cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag catgtttgct
     3721 gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg gccggttaat
     3781 gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg gcgcatgcgt
     3841 ggaattatca cctcgagaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     3901 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     3961 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tactagtcgt
     4021 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4081 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     4141 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4201 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     4261 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     4321 ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca ggggcgggcc
     4381 ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     4441 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     4501 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     4561 aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     4621 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     4681 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     4741 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     4801 aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc ggcgctcggg
     4861 agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct cagccgtcgc
     4921 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctggagctt
     4981 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     5041 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     5101 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     5161 ttttcttcca tttcaggtgt cgtgaacacg ggctgcagaa gttggtcgtg aggcactggg
     5221 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     5281 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     5341 gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg ctagagtact
     5401 taatacgact cactataggc tagcctcgag aattcaccat ggtcaacgac cctccagtac
     5461 ctgccttact gtgggcccag gaggtgggcc aagtcttggc aggccgtgcc cgcaggctgc
     5521 tgctgcagtt tggggtgctc ttctgcacca tcctcctttt gctctgggtg tctgtcttcc
     5581 tctatggatc tttttattac tcttacatgc cgacagtcag ccacctcagc cctgtgcatt
     5641 tctactacag gaccgactgt gattcctcca ccacctcact ctgctccttc cctgttgcca
     5701 atgtctcgct gactaagggt ggacgtgatc gggtgctgat gtatggacag ccgtatcgtg
     5761 ttaccttaga gcttgagctg ccagagtccc ctgtgaatca agatttgggc atgttcttgg
     5821 tcaccatttc ctgctacacc agaggtggcc gaatcatctc cacttcttcg cgttcggtga
     5881 tgctgcatta ccgctcagac ctgctccaga tgctggacac actggtcttc tctagcctcc
     5941 tgctatttgg ctttgcagag cagaagcagc tgctggaggt ggaactctac gcagactata
     6001 gagagaactc gtacgtgccg accactggag cgatcattga gatccacagc aagcgcatcc
     6061 agctgtatgg agcctacctc cgcatccacg cgcacttcac tgggctcaga tacctgctat
     6121 acaacttccc gatgacctgc gccttcatag gtgttgccag caacttcacc ttcctcagcg
     6181 tcatcgtgct cttcagctac atgcagtggg tgtggggggg catctggccc cgacaccgct
     6241 tctctttgca ggttaacatc cgaaaaagag acaattcccg gaaggaagtc caacgaagga
     6301 tctctgctca tcagccaggg cctgaaggcc aggaggagtc aactccgcaa tcagatgtta
     6361 cagaggatgg tgagagccct gaagatccct cagggacaga gggtcagctg tctgaggagg
     6421 agaaaccaga tcagcagccc ctgagcggag aagaggagct agagcctgag gccagtgatg
     6481 gttcaggctc ctgggaagat gcagctttgc tgacggaggc caacctgcct gctcctgctc
     6541 ctgcttctgc ttctgcccct gtcctagaga ctctgggcag ctctgaacct gctgggggtg
     6601 ctctccgaca gcgccccacc tgctctagtt ccggatctgg cggaggaggg agcgggggag
     6661 gcggatctgg cggaggcgga tccggcttct ctgagaccgt ggacctgatg ctgaacctgc
     6721 agtccaataa ggagggctct gtggatctga agaacgtgag cgccgtgcct aaggagaaga
     6781 ccacactgaa ggacccatcc aagccccctg ccaaggcaca ggtggtggga tggccacccg
     6841 tgcggaacta cagaaagaat atgatgaccc agcagaagac aagctcctcc ggagtgagca
     6901 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     6961 agggctccgt gaacggccac gagtttgaga tcgagggcga gggcgagggc cgcccctacg
     7021 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     7081 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     7141 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     7201 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     7261 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     7321 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     7381 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     7441 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     7501 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     7561 gccgccactc caccggcggc atggacgagc tgtacaagta gcggccgctc tagcccaatt
     7621 ccgccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     7681 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     7741 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     7801 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     7861 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     7921 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     7981 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     8041 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     8101 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     8161 ttttcctttg aaaaacacga tgataatatg ggcaccgagt acaagcccac ggtgcgcctc
     8221 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8281 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     8341 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     8401 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8461 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8521 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8581 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8641 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8701 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8761 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     8821 gaaaggagcg cacgacccca tggctccgac cgaagccacc cggggcggcc ccgccgaccc
     8881 cgcacccgcc cccgaggccc accgactcta gctagaggat cataatcagc cataccacat
     8941 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     9001 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     9061 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9121 tgtccatgca tcactaggga caggattggt gacagaaaag ccccatcctt aggcctcctc
     9181 cttcctagtc tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct
     9241 ggtgacacac ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc
     9301 ttacgatgga gccagagagg atcctgggag ggagagcttg gcagggggtg ggagggaagg
     9361 gggggatgcg tgacctgccc ggttctcagt ggccaccctg cgctaccctc tcccagaacc
     9421 tgagctgctc tgacgcggct gtctggtgcg tttcactgat cctggtgctg cagcttcctt
     9481 acacttccca agaggagaag cagtttggaa aaacaaaatc agaataagtt ggtcctgagt
     9541 tctaactttg gctcttcacc tttctagtcc ccaatttata ttgttcctcc gtgcgtcagt
     9601 tttacctgtg agataaggcc agtagccagc cccgtcctgg cagggctgtg gtgaggaggg
     9661 gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt tcaccaggtc
     9721 gtggccgcct ctactccctt tctctttctc catccttctt tccttaaaga gtccccagtg
     9781 ctatctggga catattcctc cgcccagagc agggtcccgc ttccctaagg ccctgctctg
     9841 ggcttctggg tttgagtcct tggcaagccc aggagaggcg ctcaggcttc cctgtccccc
     9901 ttcctcgtcc accatctcat gcccctggct ctcctgcccc ttccctacag gggttcctgg
     9961 ctctgctctt cagactgagc cccgttcccc tgcatccccg ttcccctgca tcccccttcc
    10021 cctgcatccc ccagaggccc caggccacct acttggcctg gaccccacga gaggccaccc
    10081 cagccctgtc taccaggctg ccttttgggt ggattctcct ccaactgtgg ggtgactgct
    10141 tggcaaactc actcttcggg gtatcccagg aggcctggag cattggggtg ggctggggtt
    10201 cagagaggag ggattccctt ctcaggttac gtggccaaga agcaggggag ctgggtttgg
    10261 gtcaggtctg ggtgtggggt gaccagctta tgctgtttgc ccaggacagc ctagttttag
    10321 cgctgaaacc ctcagtccta ggaaaacagg gatggttggt cactgtctct gggctcgac
//
