LOCUS       sequence-254834-        7502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression human MSH2 protein using baculovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Geng H, Du C, Chen S, Salerno V, Manfredi C, Hsieh P
  TITLE     In vitro studies of DNA mismatch repair proteins.
  JOURNAL   Anal Biochem. 2011 Jun 15;413(2):179-84. doi: 
            10.1016/j.ab.2011.02.017. Epub 2011 Feb 15.
  PUBMED    21329650
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2011.02.017"; journalName: "Anal Biochem"; date: 
            "2011-06-15- 15"; volume: "413"; issue: "2"; pages: "179-84"
COMMENT     Sequence Label: pFB1_hMSH2
FEATURES             Location/Qualifiers
     source          1..7502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1014..1019,1020..1036,1037..1042))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1394..1485
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1410..1427
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4482..4616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4519..4538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4573..4592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4645..4810
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4994..5448
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5081..5100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5290..5311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5474..5578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5579..5647,5648..6439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5797..5816)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6610..7198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7099..7118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7352..7369
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
      841 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
      901 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
      961 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     1021 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     1081 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     1141 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     1201 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     1261 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     1321 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc
     1381 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     1441 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     1501 taccgtccca ccatcgggcg cggatccaca tggcggtgca gccgaaggag acgctgcagt
     1561 tggagagcgc ggccgaggtc ggcttcgtgc gcttctttca gggcatgccg gagaagccga
     1621 ccaccacagt gcgccttttc gaccggggcg acttctatac ggcgcacggc gaggacgcgc
     1681 tgctggccgc ccgggaggtg ttcaagaccc agggggtgat caagtacatg gggccggcag
     1741 gagcaaagaa tctgcagagt gttgtgctta gtaaaatgaa ttttgaatct tttgtaaaag
     1801 atcttcttct ggttcgtcag tatagagttg aagtttataa gaatagagct ggaaataagg
     1861 catccaagga gaatgattgg tatttggcat ataaggcttc tcctggcaat ctctctcagt
     1921 ttgaagacat tctctttggt aacaatgata tgtcagcttc cattggtgtt gtgggtgtta
     1981 aaatgtccgc agttgatggc cagagacagg ttggagttgg gtatgtggat tccatacaga
     2041 ggaaactagg actgtgtgaa ttccctgata atgatcagtt ctccaatctt gaggctctcc
     2101 tcatccagat tggaccaaag gaatgtgttt tacccggagg agagactgct ggagacatgg
     2161 ggaaactgag acagataatt caaagaggag gaattctgat cacagaaaga aaaaaagctg
     2221 acttttccac aaaagacatt tatcaggacc tcaaccggtt gttgaaaggc aaaaagggag
     2281 agcagatgaa tagtgctgta ttgccagaaa tggagaatca ggttgcagtt tcatcactgt
     2341 ctgcggtaat caagttttta gaactcttat cagatgattc caactttgga cagtttgaac
     2401 tgactacttt tgacttcagc cagtatatga aattggatat tgcagcagtc agagccctta
     2461 acctttttca gggttctgtt gaagatacca ctggctctca gtctctggct gccttgctga
     2521 ataagtgtaa aacccctcaa ggacaaagac ttgttaacca gtggattaag cagcctctca
     2581 tggataagaa cagaatagag gagagattga atttagtgga agcttttgta gaagatgcag
     2641 aattgaggca gactttacaa gaagatttac ttcgtcgatt cccagatctt aaccgacttg
     2701 ccaagaagtt tcaaagacaa gcagcaaact tacaagattg ttaccgactc tatcagggta
     2761 taaatcaact acctaatgtt atacaggctc tggaaaaaca tgaaggaaaa caccagaaat
     2821 tattgttggc agtttttgtg actcctctta ctgatcttcg ttctgacttc tccaagtttc
     2881 aggaaatgat agaaacaact ttagatatgg atcaggtgga aaaccatgaa ttccttgtaa
     2941 aaccttcatt tgatcctaat ctcagtgaat taagagaaat aatgaatgac ttggaaaaga
     3001 agatgcagtc aacattaata agtgcagcca gagatcttgg cttggaccct ggcaaacaga
     3061 ttaaactgga ttccagtgca cagtttggat attactttcg tgtaacctgt aaggaagaaa
     3121 aagtccttcg taacaataaa aactttagta ctgtagatat ccagaagaat ggtgttaaat
     3181 ttaccaacag caaattgact tctttaaatg aagagtatac caaaaataaa acagaatatg
     3241 aagaagccca ggatgccatt gttaaagaaa ttgtcaatat ttcttcaggc tatgtagaac
     3301 caatgcagac actcaatgat gtgttagctc agctagatgc tgttgtcagc tttgctcacg
     3361 tgtcaaatgg agcacctgtt ccatatgtac gaccagccat tttggagaaa ggacaaggaa
     3421 gaattatatt aaaagcatcc aggcatgctt gtgttgaagt tcaagatgaa attgcattta
     3481 ttcctaatga cgtatacttt gaaaaagata aacagatgtt ccacatcatt actggcccca
     3541 atatgggagg taaatcaaca tatattcgac aaactggggt gatagtactc atggcccaaa
     3601 ttgggtgttt tgtgccatgt gagtcagcag aagtgtccat tgtggactgc atcttagccc
     3661 gagtaggggc tggtgacagt caattgaaag gagtctccac gttcatggct gaaatgttgg
     3721 aaactgcttc tatcctcagg tctgcaacca aagattcatt aataatcata gatgaattgg
     3781 gaagaggaac ttctacctac gatggatttg ggttagcatg ggctatatca gaatacattg
     3841 caacaaagat tggtgctttt tgcatgtttg caacccattt tcatgaactt actgccttgg
     3901 ccaatcagat accaactgtt aataatctac atgtcacagc actcaccact gaagagacct
     3961 taactatgct ttatcaggtg aagaaaggtg tctgtgatca aagttttggg attcatgttg
     4021 cagagcttgc taatttccct aagcatgtaa tagagtgtgc taaacagaaa gccctggaac
     4081 ttgaggagtt tcagtatatt ggagaatcgc aaggatatga tatcatggaa ccagcagcaa
     4141 agaagtgcta tctggaaaga gagcaaggtg aaaaaattat tcaggagttc ctgtccaagg
     4201 tgaaacaaat gccctttact gaaatgtcag aagaaaacat cacaataaag ttaaaacagc
     4261 taaaagctga agtaatagca aagaataata gctttgtaaa tgaaatcatt tcacgaataa
     4321 aagttactac gtgactcgag gcatgcggta ccaagcttgt cgagaagtac tagaggatca
     4381 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4441 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4501 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4561 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     4621 cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
     4681 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     4741 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     4801 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
     4861 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
     4921 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
     4981 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5041 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5101 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5161 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5221 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5281 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5341 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5401 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5581 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5641 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5701 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5761 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5821 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5881 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5941 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6001 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6061 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6121 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6181 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6241 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6301 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6361 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6421 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7261 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7321 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7381 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     7441 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     7501 gg
//
