LOCUS       sequence-254836-        9222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible N2H assay destination vector with N-terminus 
            tagging of nanoluc fragment2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, 
            Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan 
            S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker 
            EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal 
            M, Calderwood MA, Jacob Y
  TITLE     Maximizing binary interactome mapping with a minimal number of 
            assays.
  JOURNAL   Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2.
  PUBMED    31467278
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-29"; volume: "10(1):3907. doi"; pages: " 
            10.1038/s41467-019-11809-2"
COMMENT     Sequence Label: pDEST-N2H-N2
FEATURES             Location/Qualifiers
     source          1..9222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     757..776
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1323..1447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1472..1477,1478..1495,1496..1502)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1556..2215
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1622..1641)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2557..2862
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2741..2760
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2903..3027)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3065..3098
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3379..3500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3390..3409)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3444..3463
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      3565..3752
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3620..3644)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     rep_origin      3769..3904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4003..4020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4174..4762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4254..4273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4933..5724,5725..5793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5556..5575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5794..5898)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5925..7267
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6888..6935)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    7290..7323
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7354..7372
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7410..7432)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7532..7551
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7572..7853
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7854..8528
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8631..9086)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8768..8789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8980..8999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgg gcgcgcccct
      541 ccttgaatta attaaggcgc gccactgaaa tttttaatta aggcgcgcct agcatgtgat
      601 taattaagca tgtctttaga tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
      661 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc caggtccact tcgcatataa
      721 aaggtgacgc gtgtggatcc tcgaacaccg agctcttaat acgactcact atagggaaag
      781 caaaaatgtg atcttgcttg taaatacaat tttgagaggt taataaatta caagtagtgc
      841 tatttttgta tttaggttag ctatttagct ttacgttcca ggatgcctag tggcagcccc
      901 acaatatcca ggaagccctc tctgcggttt ttcagattag gtagtcgaaa aacctaagaa
      961 atttacctat gggcctgagc ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg
     1021 tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca ctggtaatcg
     1081 acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc atcgccgtgt
     1141 tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa attatcgacg
     1201 agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac ggagtgaccg
     1261 gctggcggct gtgcgaacgc attctggcgg gagggggcag tggtggaggg ggcagtccca
     1321 tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
     1381 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
     1441 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     1501 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
     1561 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     1621 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     1681 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1741 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     1801 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     1861 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     1921 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     1981 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     2041 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     2101 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     2161 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg
     2221 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     2281 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     2341 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     2401 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     2461 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     2521 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     2581 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     2641 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     2701 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     2761 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     2821 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     2881 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     2941 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     3001 tcgttcagct ttcttgtaca aagtggtgta agtaagtaac tataacggtc ctaaggtagc
     3061 gaacataact tcgtataatg tatgctatac gaagttatgg aaaaaaaaaa aaaaaaaaaa
     3121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3301 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3361 aaaaaagaga agacatgata agatacattg atgagtttgg acaaaccaca acaagaatgc
     3421 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3481 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3541 gggagatgtg ggaggttttt taaagcgaat ttcttatgat ttatgatttt tattattaaa
     3601 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     3661 aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
     3721 gaggtcgctc ttattgacca cacctctacc ggcggcctaa ctccgcccat cccgccccta
     3781 actccgccca gttccgccca ttctccgcct catggctgac taattttttt tatttatgca
     3841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggacg cttttttgga
     3901 ggccgaggct tttgcaaaga tcgaacaaga gacaggactg cattaatgaa tcggccaacg
     3961 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4021 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4081 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4141 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4261 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4381 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4501 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4561 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4621 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4681 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4741 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4861 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4921 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4981 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5041 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5101 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5161 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5221 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5281 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5341 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5401 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5461 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5521 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5581 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5641 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5701 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5761 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5821 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5881 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa gcatctgtgc
     5941 ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     6001 gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct
     6061 gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     6121 tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag
     6181 aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa
     6241 agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact
     6301 ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc
     6361 cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc
     6421 attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact
     6481 ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt
     6541 tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc
     6601 gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat
     6661 aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg
     6721 gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt
     6781 ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt
     6841 ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac
     6901 tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt
     6961 ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg
     7021 cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa
     7081 tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc tcctgtgata
     7141 ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt agctgttcta
     7201 tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata
     7261 ttggatcata ctaagaaacc attggtacca taacttcgta taaggtatac tatacgaagt
     7321 tatgctagca ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     7381 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7441 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7501 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     7561 caccatagat caacgacatt actatatata taatatagga agcatttaat agaacagcat
     7621 cgtaatatat gtgtactttg cagttatgac gccagatggc agtagtggaa gatattcttt
     7681 attgaaaaat agcttgtcac cttacgtaca atcttgatcc ggagcttttc tttttttgcc
     7741 gattaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga gggcattggt
     7801 gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg agtatgtctg
     7861 ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg cagagcacag
     7921 aggccgcaga atgtgctcta gattccgatg ctgacttgct gggtattata tgtgtgccca
     7981 atagaaagag aacaattgac ccggttattg caaggaaaat ttcaagtctt gtaaaagcat
     8041 ataaaaatag ttcaggcact ccgaaatact tggttggcgt gtttcgtaat caacctaagg
     8101 aggatgtttt ggctctggtc aatgattacg gcattgatat cgtccaactg catggagatg
     8161 agtcgtggca agaataccaa gagttcctcg gtttgccagt tattaaaaga ctcgtatttc
     8221 caaaagactg caacatacta ctcagtgcag cttcacagaa acctcattcg tttattccct
     8281 tgtttgattc agaagcaggt gggacaggtg aacttttgga ttggaactcg atttctgact
     8341 gggttggaag gcaagagagc cccgaaagct tacattttat gttagctggt ggactgacgc
     8401 cagaaaatgt tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag
     8461 gtgtggagac aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta
     8521 agaaataggt tattactgag tagtatttat ttaagtattg tttgtgcact tgccgatcta
     8581 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag
     8641 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     8701 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     8761 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     8821 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     8881 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     8941 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     9001 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     9061 gcttaatgcg ccgctacagg gcgcgtcaat attggccatt agccatatta ttcattggtt
     9121 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     9181 tgtacattta tattggctca tgtccaatat gaccgccatg tt
//
