LOCUS       sequence-254837-       11517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible N2H assay destination vector with C-terminus 
            tagging of nanoluc fragment1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, 
            Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan 
            S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker 
            EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal 
            M, Calderwood MA, Jacob Y
  TITLE     Maximizing binary interactome mapping with a minimal number of 
            assays.
  JOURNAL   Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2.
  PUBMED    31467278
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-29"; volume: "10(1):3907. doi"; pages: " 
            10.1038/s41467-019-11809-2"
COMMENT     Sequence Label: pDEST-N2H-C1
FEATURES             Location/Qualifiers
     source          1..11517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     757..776
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    978..1102
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1127..1132,1133..1150,1151..1157)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1211..1870
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1277..1296)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3550..3855
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3734..3753
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3896..4020)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4293..4326)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4467..4588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4478..4497)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4532..4551
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4653..4840
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4708..4732)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(4845..4878)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     rep_origin      4893..5028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5127..5144)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5298..5886)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5378..5397)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6057..6848,6849..6917))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6680..6699
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6918..7022)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7049..8391
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8012..8059)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8432..8450
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8488..8510)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8610..8629
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(9123..10217)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(10218..10625)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(10926..11381)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11063..11084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11275..11294
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgg gcgcgcccct
      541 ccttgaatta attaaggcgc gccactgaaa tttttaatta aggcgcgcct agcatgtgat
      601 taattaagca tgtctttaga tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
      661 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc caggtccact tcgcatataa
      721 aaggtgacgc gtgtggatcc tcgaacaccg agctcttaat acgactcact atagggaaag
      781 caaaaatgtg atcttgcttg taaatacaat tttgagaggt taataaatta caagtagtgc
      841 tatttttgta tttaggttag ctatttagct ttacgttcca ggatgcctag tggcagcccc
      901 acaatatcca ggaagccctc tctgcggttt ttcagattag gtagtcgaaa aacctaagaa
      961 atttacctat gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     1021 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     1081 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     1141 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     1201 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     1261 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1321 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     1381 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     1441 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     1501 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     1561 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     1621 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     1681 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     1741 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1801 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     1861 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     1921 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     1981 aggtatgcta tgaagcactg atgaatcccc taatgatttt ggtaaaaatc attaagttaa
     2041 ggtggataca catcttgtca tatgatcaaa tggtttcgcg aaaaatcaat aatcagacaa
     2101 caagatgtgc gaactcgata ttttacacga ctctctttac caattctgcc ccgaattaca
     2161 cttaaaacga ctcaacagct taacgttggc ttgccacgca ttacttgact gtaaaactct
     2221 cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa
     2281 acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg ctgtataccg
     2341 ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg ttgactggtc
     2401 tgatattcgt gagcaaaaac gacttatggt attgcgagct tcagtcgcac tacacggtcg
     2461 ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa agaaagctca
     2521 tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac cgctcattgt
     2581 cagtgatgct ggctttaaag tgccatggta taaatccgtt gagaagctgg gttggtactg
     2641 gttaagtcga gtaagaggaa aagtacaata tgcagaccta ggagcggaaa actggaaacc
     2701 tatcagcaac ttacatgata tgtcatctag tcactcaaag actttaggct ataagaggct
     2761 gactaaaagc aatccaatct catgccaaat tctattgtat aaatctcgct ctaaaggccg
     2821 aaaaaatcag cgctcgacac ggactcattg tcaccacccg tcacctaaaa tctactcagc
     2881 gtcggcaaag gagccatggg ttctagcaac taacttacct gttgaaattc gaacacccaa
     2941 acaacttgtt aatatctatt cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa
     3001 aagtcctgcc tacggactag gcctacgcca tagccgaacg agcagctcag agcgttttga
     3061 tatcatgctg ctaatcgccc tgatgcttca actaacatgt tggcttgcgg gcgttcatgc
     3121 tcagaaacaa ggttgggaca agcacttcca ggctaacaca gtcagaaatc gaaacgtact
     3181 ctcaacagtt cgcttaggca tggaagtttt gcggcattct ggctacacaa taacaaggga
     3241 agacttactc gtggctgcaa ccctactagc tcaaaattta ttcacacatg gttacgcttt
     3301 ggggaaatta tgaggggatc tctcagtatg aagcagcgta ttacagtgac agttgacagc
     3361 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa
     3421 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag
     3481 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg
     3541 gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
     3601 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc
     3661 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
     3721 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
     3781 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
     3841 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
     3901 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     3961 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
     4021 gatgggtgga gggggcagtg gtggaggggg cagtgtcttc acactcgaag atttcgttgg
     4081 ggactggcga cagacagccg gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc
     4141 cagtttgttt cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg
     4201 tgaaaatggg ctgaagatcg acatccatgt catcatcccg tatgaatagt agtagtagtg
     4261 ataataacta taacggtcct aaggtagcga acataacttc gtatagcata cattatacga
     4321 agttatggaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4381 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4441 aaaaaaaaaa aaaagagaag acatgataag atacattgat gagtttggac aaaccacaac
     4501 aagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     4561 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     4621 ggttcagggg gagatgtggg aggtttttta aagcgaattt cttatgattt atgattttta
     4681 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     4741 taaaacgaaa attcttgttc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     4801 tatagcatga ggtcgctctt attgaccaca cctctaccgg cggaataact tcgtatagta
     4861 taccttatac gaagttatgc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4921 cccattctcc gcctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4981 cggcctctga gctattccag aagtagtgag gacgcttttt tggaggccga ggcttttgca
     5041 aagatcgaac aagagacagg actgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5101 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5161 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5221 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5281 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5341 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5401 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5461 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5521 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5581 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5641 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5701 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5761 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5821 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5881 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5941 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6001 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6061 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6121 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6181 gctgcaatga taccgcgaga tccacgctca ccggctccag atttatcagc aataaaccag
     6241 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6301 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6361 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6421 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6481 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6541 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6601 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6661 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6721 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6781 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6841 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6901 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6961 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7021 cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca
     7081 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     7141 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     7201 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     7261 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     7321 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     7381 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     7441 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     7501 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     7561 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     7621 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     7681 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     7741 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     7801 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     7861 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     7921 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     7981 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga
     8041 acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg
     8101 agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat
     8161 atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg
     8221 tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc
     8281 ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc
     8341 aattggatta gtctcatcct tcaatgctat catttccttt gatattggat catactaaga
     8401 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8461 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8521 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8581 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8641 ccatatcgac tacgtcgtta aggccgtttc tgacagagta aaattcttga gggaactttc
     8701 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
     8761 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatataaa
     8821 ttaggaatca tagtttcatg attttctgtt acacctaact ttttgtgtgg tgccctcctc
     8881 cttgtcaata ttaatgttaa agtgcaattc tttttcctta tcacgttgag ccattagtat
     8941 caatttgctt acctgtattc ctttacatcc tcctttttct ccttcttgat aaatgtatgt
     9001 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
     9061 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
     9121 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
     9181 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
     9241 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
     9301 aagatagtgg cgatagggtt gaccttattc tttggcaaat ctggagcaga accgtggcat
     9361 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
     9421 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     9481 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     9541 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     9601 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     9661 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     9721 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     9781 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     9841 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     9901 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     9961 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc agccttcttg
    10021 gaggcttcca gcgcctcatc tggaagtgga acacctgtag catcgatagc agcaccacca
    10081 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
    10141 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
    10201 ttcttagggg cagacattag aatggtatat ccttgaaata tatatatata tattgctgaa
    10261 atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat tggaaaaaac
    10321 aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat ttagtcatga
    10381 acgcttctct attctatatg aaaagccggt tccggcgctc tcacctttcc tttttctccc
    10441 aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc
    10501 cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa
    10561 aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg agtattccca
    10621 cagttaactg cggtcaagat atttcttgaa tcaggcgcct tagaccgctc ggccaaacaa
    10681 ccaattactt gttgagaaat agagtataat tatcctataa atataacgtt tttgaacaca
    10741 catgaacaag gaagtacagg acaattgatt ttgaagagaa tgtggatttt gatgtaattg
    10801 ttgggattcc atttttaata aggcaataat attaggtatg tagatatact agaagttctc
    10861 ctcgaccggt cgatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10921 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
    10981 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
    11041 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
    11101 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
    11161 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
    11221 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
    11281 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
    11341 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaatattgg ccattagcca
    11401 tattattcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
    11461 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgtt
//
