LOCUS       sequence-254846-        7733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human oligoadenylate synthase 1 (OAS1) is used to label terminal 
            ADP-riobos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ando Y, Elkayam E, McPherson RL, Dasovich M, Cheng SJ, Voorneveld J,
            Filippov DV, Ong SE, Joshua-Tor L, Leung AKL
  TITLE     ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):845-856.e5. doi: 
            10.1016/j.molcel.2018.12.022. Epub 2019 Jan 31.
  PUBMED    30712989
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.022"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "845-856.e5"
COMMENT     Sequence Label: pFl Strep-Sumo-TEV human OAS1
FEATURES             Location/Qualifiers
     source          1..7733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(134..139,140..163,164..199,200..223)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             239..526
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             539..559
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1939..2073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1976..1995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2030..2049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2183..2348
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2532..2986
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2619..2638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2828..2849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3012..3116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3117..3185,3186..3977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3335..3354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4148..4736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4637..4656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4890..4907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5041..5265
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5332..5865)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6035..6040,6041..6057,6058..6063))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(6459..6492)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6682..6730
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6755..6774
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(6819..7532,7533..7535,7536..7538))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6864..6885)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7213..7232
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7472..7493
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(7580..7689)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atgtctgctt ggtcacaccc tcaattcgag aaaggcggcg gtagtggcgg
      181 cggatctggc ggtagtgcct ggagccatcc gcagtttgaa aagaccggtt ccctgcagga
      241 ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca
      301 catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac
      361 tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt
      421 aacattcttg tacgacggta ttgaaattca agctgatcag acccctgaag atttggacat
      481 ggaggataac gatattattg aggctcacag agaacagatt ggtggaatcc caacgaccga
      541 aaacctgtat tttcagggcg ccatgatgga cctgcgtaac accccggcga agagcctgga
      601 caagtttatt gaggattatc tgctgccgga cacctgcttt cgtatgcaaa tcaaccacgc
      661 gattgacatc atttgcggct tcctgaaaga gcgttgcttt cgtggtagca gctacccggt
      721 gtgcgttagc aaagtggtta agggtggcag cagcggtaaa ggtaccaccc tgcgtggccg
      781 tagcgatgcg gatctggtgg ttttcctgag cccgctgacc acctttcagg atcaactgaa
      841 ccgtcgtggt gagttcatcc aggaaattcg tcgtcaactg gaggcgtgcc agcgtgaacg
      901 tgcgttcagc gtgaaatttg aagttcaagc gccgcgttgg ggtaacccgc gtgcgctgag
      961 ctttgtgctg agcagcctgc agctgggcga gggtgtggag ttcgacgttc tgccggcgtt
     1021 tgatgcgctg ggccaactga ccggtggcta caaaccgaac ccgcagatct atgttaagct
     1081 gattgaggaa tgcaccgacc tgcaaaaaga gggtgagttc agcacctgct ttaccgaact
     1141 gcaacgtgat ttcctgaagc agcgtccgac caaactgaag agcctgatcc gtctggtgaa
     1201 acactggtac caaaactgca agaaaaagct gggcaagctg ccgccgcagt atgcgctgga
     1261 gctgctgacc gtttatgcgt gggaacgtgg cagcatgaaa acccacttca acaccgcgca
     1321 aggttttcgt accgtgctgg agctggttat caactaccag caactgtgca tctactggac
     1381 caagtactac gacttcaaga acccgatcat tgaaaaatac ctgcgtcgtc agctgaccaa
     1441 accgcgtccg gtgattctgg acccggcgga cccgaccggt aacctgggtg gcggtgaccc
     1501 gaaaggttgg cgtcagctgg cgcaagaggc ggaagcgtgg ctgaactatc cgtgcttcaa
     1561 gaactgggat ggtagcccgg ttagcagctg gattctgctg gcggagagca acagcgcgga
     1621 cgatgaaacc gacgatccgc gtcgttacca gaagtacggc tatattggta cccacgaata
     1681 tccgcacttt agccaccgtc cgagcaccct gcaagcggcg agcaccccgc aagcggaaga
     1741 agactggacc tgcaccatcc tgtgatagga tcgaatcgga ctttcgaatc tagagcctgc
     1801 agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
     1861 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1921 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2041 aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc
     2101 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     2161 atcataaccc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc
     2221 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     2281 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     2341 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc
     2401 atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt
     2461 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa
     2521 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2581 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2641 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2701 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2761 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2821 cttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2881 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2941 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     3001 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3061 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3121 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3181 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3241 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3301 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3361 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3421 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3481 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3541 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3601 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3661 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3721 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3781 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3841 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3901 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3961 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4021 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4261 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4921 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc
     4981 cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg
     5041 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
     5101 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
     5161 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
     5221 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
     5281 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
     5341 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     5401 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     5461 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     5521 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     5581 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     5641 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     5701 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     5761 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     5821 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     5881 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
     5941 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     6001 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     6061 caactgggtt tgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     6121 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     6181 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     6241 gcccttgctt caggagatcg gtagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     6301 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     6361 ggactctagc tatagttcta gtggttggct acagctttgt ttgtactatc aacaggttga
     6421 actgctgatc aacagatcct ctacgcggcc gcggtaccat aacttcgtat agcatacatt
     6481 atacgaagtt atctggttta aacgtacccg tagtggctat ggcagggctt gccgccccga
     6541 cgttggctgc gagccctggg ccttcacccg aacttggggg ttggggtggg gaaaaggaag
     6601 aaacgcgggc gtattggtcc caatggggtc tcggtggggt atcgacagag tgccagccct
     6661 gggaccgaac cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt
     6721 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
     6781 ctcccccatc tcccggtacc gcatgctccg attcgatcct acttgtacag ctcgtccatg
     6841 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
     6901 ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg cagcagcacg
     6961 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
     7021 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
     7081 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
     7141 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
     7201 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
     7261 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
     7321 gcgaagcact gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc
     7381 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
     7441 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
     7501 accaccccgg tgaacagctc ctcgcccttg ctcaccatca tggtttcgga ccgcccgggt
     7561 gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt tacagtatag tattttaatt
     7621 aatatacaaa tgatttgata ataattctta tttaactata atatattgtg ttgggttgaa
     7681 ttaaaggtcc gtatactagt atcgattcgc gacctactcc ggaatattaa tag
//
