LOCUS       sequence-254849-        9994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  System with AIP and xylose inducible agrCA sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Gonzalez LM, Mukhitov N, Voigt CA
  TITLE     Resilient living materials built by printing bacterial spores.
  JOURNAL   Nat Chem Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 
            10.1038/s41589-019-0412-5.
  PUBMED    31792444
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 
            10.1038/s41589-019-0412-5."
COMMENT     Sequence Label: pNM58
FEATURES             Location/Qualifiers
     source          1..9994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      129..674
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     575..594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     791..808
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1519..2229
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1546..1574)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2169..2189
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2248..2266)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2262..2309
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(6190..6975,6976..7044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKH"
     primer_bind     6807..6826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7045..7149)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7217..7235
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(7313..8689)
                     /codon_start=1
                     /gene="Bacillus subtilis tetL"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(8889..9893)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
        1 acacttcaac gcacctttca gcaacccttt ggtattattt ttttgtgtgg aaaatacggc
       61 tatgggagaa gacttgctca ccaaatttcg ttccactgag cgtcagaccc cttaataaga
      121 tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc
      181 gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac
      241 tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat
      301 gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc
      361 atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg
      421 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
      481 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg
      541 caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc
      601 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
      661 ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct
      721 ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac
      781 gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg
      841 ctgacgcacc ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca
      901 tcagtgccaa catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgcag
      961 cgagtcagtg agttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtggaga
     1021 gcaagcccgt agtaaaataa aaatgacgtt atttctatat gtatcaagat aagaaagaac
     1081 aagttcaaaa ccatcaaaaa aagacacctt ttcaggtgct ttttttattt tataaactca
     1141 ttccctgatc tcgacttcgt tcttttttta cctctcggtt atgagttagt tcaaattcgt
     1201 tctttttagg ttctaaatcg tgtttttctt ggaattgtgc tgttttatcc tttaccttgt
     1261 ctacaaaccc cttaaaaacg tttttaaagg cttttaagcc gtctgtacgt tccttaaggc
     1321 ctaactgtag gaaataaata cttaactgtt aaatgtaatt tgtatttaat attttaacat
     1381 aaaaaaattt acagttaaga ataaaaaacg actagttaag aaaaattgga aaataaatgc
     1441 ttttagcatg ttttaatata actagatcac agagatgtgg tcagtcagtc ttaattaacc
     1501 aaggaggtga aatgtacaat gaaaggagaa gaacttttca ctggagttgt cccaattctt
     1561 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     1621 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1681 ccatggccaa cacttgtcac tactttcgcg tatggtcttc aatgctttgc gagataccca
     1741 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     1801 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     1861 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     1921 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     1981 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     2041 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2101 ccagacaacc attacctgtc cacacaatct aagctttcga aagatcccaa cgaaaagaga
     2161 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2221 ctatacaaat aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     2281 cctctaaacg ggtcttgagg ggttttttgt aaaaatatcc cactttatcc aattttcgtt
     2341 tgttgaacta atgggtgctt tagttgaaga ataaaagacc acattaaaaa atgtggtctt
     2401 ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt atcttgataa
     2461 taagggtaac tattgccgga tcgtcctcag gagtaggcga catcgctaaa taatgatcta
     2521 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccctttgc
     2581 gttcacttaa ctaacttata ggggtaacac ttaaaaaaga atcaataacg atagaaaccg
     2641 ctcctaaagc aggtgcattt tttcctaacg aagaaggcaa tagttcacat ttattgtcta
     2701 aatgagaatg gactctagaa gaaacttcgt ttttaatcgt atttaaaaca atgggatgag
     2761 attcaattat atgatttctc aagataacag cttctatatc aaatgtatta aggatattgg
     2821 ttaatccaat tccgatataa aagccaaagt tttgaagtgc atttaacatt tctacatcat
     2881 ttttatttgc gcgttccaca atctcttttc gagaaatatt cttttcttct ttagagagcg
     2941 aagccagtaa cgctttttca gaagcatata attcccaaca gcctcgattt ccacagctgc
     3001 atttgggtcc attaaaatct atcgtcatat gacccatttc cccagaaaaa ccctgaacac
     3061 ctttatacaa ttcgttgtta ataacaagtc cagttccaat tccgatatta atactgatgt
     3121 aaacgatgtt ttcatagttt tttgtcatac caaatacttt ttcaccgtat gctcctgcat
     3181 tagcttcatt ttcaacaaaa accggaacat taaactcact ctcaattaaa aactgcaaat
     3241 ctttgatatt ccaatttaag ttaggcatga aaataatttg ctgatgacga tctacaaggc
     3301 ctggaacaca aattcctatt ccgactagac cataagggga ctcaggcata tgggttacaa
     3361 aaccatgaat aagtgcaaat aaaatctctt ttacttcact agcggaagaa ctagacaagt
     3421 cagaagtctt ctcgagaata atatttcctt ctaagtcggt tagaattccg ttaagatagt
     3481 cgactcctat atcaatacca atcgagtagc ctgcattctt attaaaaaca agcattacag
     3541 gtcttctgcc gcctctagat tgccctgccc caatttcaaa aataaaatct ttttcaagca
     3601 gtgtatttac ttgagaggag acagtagact tgtttaatcc tgtaatctca gagagagttg
     3661 ccctggagac aggggagttc ttcaaaattt catctaatat taatttttga ttcatttttt
     3721 ttactaaagc ttgatctgca atttgaataa taaccactcc tttgtttatc caccgaacta
     3781 agttggtgtt ttttgaagct tgaattagat atttaaaagt atcatatcta atattataac
     3841 taaattttct aaaaaaaaca ttgaaataaa catttatttt gtatatgacg agataaagtt
     3901 agtttattgg ataaacaaac taactcaatt aagatagttg atggataaac ttgttcactt
     3961 aaatcaaagg gggaaatgac aaatggtcca aactagtatg gaattgttaa acagttacaa
     4021 ctttgttttg ttcgtattaa ctcaaatgat attaatgttt acaataccag ctataattag
     4081 tggtattaag tacagtaaac ttgattattt tttcatcata gtaatttcga cattatcgtt
     4141 atttctattt aaaatgtttg atagcgcgtc cttaatcata ttaacttcat ttattattat
     4201 aatgtatttt gtcaaaatca aatggtattc tattttgttg attatgactt cgcagattat
     4261 tctatactgt gctaactaca tgtatatagt tatatatgca tatatcacca aaatttctga
     4321 tagtatattt gtaatattcc ctagcttttt tgtagtttat gtgactatta gtatactatt
     4381 ctcatatata ataaatagag ttctcaaaaa aattagcaca ccatatctaa tactaaacaa
     4441 aggattttta atagttattt cgactatctt actgcttact ttttcattat ttttctttta
     4501 ttcacaaata aactcggatg aagctaaagt aataaggcag tattctttta tttttattgg
     4561 tatcactata tttttaagta tattaacatt tgttatttct caatttctcc ttaaagagat
     4621 gaaatataaa cgtaatcaag aagaaattga aacctattat gaatatacat tgaagattga
     4681 agctatcaac aacgaaatgc gcaagttccg tcatgattat gtcaatatct taacgacact
     4741 ttcagaatac attcgagaag atgacatgcc tggcctacgt gattatttca ataaaaatat
     4801 tgtacctatg aaagacaatt tacaaatgaa tgctataaaa ttaaatggta tcgagaatct
     4861 taaagtacgt gaaattaaag gcttaattac tgcgaaaatt ttacgtgcac aagaaatgaa
     4921 tattccgatt agtatcgaaa tacccgatga agtaagtagc attaacttga atatgatcga
     4981 tttaagtcgc agtattggta ttattcttga taatgcaatt gaggcatcaa ctgaaattga
     5041 tgaccctatc attcgcgttg catttattga aagtgaaaat tcagtaacgt ttattgttat
     5101 gaataaatgc gctgatgata taccacgcat tcatgaattg ttccaagaaa gtttttctac
     5161 taaaggtgaa ggtcgtggtt taggtctatc aactttaaaa gaaattgctg ataatgcaga
     5221 caatgtctta ttagatacaa ttatcgaaaa tggtttcttt attcaaaaag ttgaaattat
     5281 taacaactag ccataaggat gtgaatgtat gaaaattttc atttgcgaag acgatccaaa
     5341 acaaagagaa aacatggtta ccattattaa aaattatata atgatagaag aaaagcctat
     5401 ggaaattgcc ctcgcaactg ataatcctta tgaggtgctt gagcaagcta aaaatatgaa
     5461 tgacataggc tgttactttt tagatattca actttcaacc gatattaatg gtatcaaatt
     5521 aggcagtgaa attcgtaagc atgacccagt tggtaacatt attttcgtta cgagtcacag
     5581 tgaacttacc tatttaacat ttgtctacaa agttgcagcg atggatttta tttttaaaga
     5641 tgatccagct gaattaagaa ctcgaattat agactgttta gaaactgcac atacacgctt
     5701 acaattgttg tctaaagata atagcgttga aacgattgaa ttaaaacgtg gcagtaattc
     5761 agtgtatgtt caatatgatg atattatgtt ttttgaatca tcaacaaaat ctcacagact
     5821 cattgcccat ttagataacc gtcaaattga attttatggt aatttaaaag aactgagtca
     5881 attagatgat cgtttcttta gatgtcataa tagctttgtc gtcaatcgcc ataatattga
     5941 atctatagat tcgaaagagc gaattgtcta ttttaaaaat aaagaacact gctatgcatc
     6001 ggtgagaaac gttaaaaaaa tataataaga taataaagtc agttaacggc gtattcaatt
     6061 gtggatcctc tagagtcatt tatattcaac tgttaactaa aactgtctgc aaattaatca
     6121 atgtctctag taaaattccg attttatacc cactcctatt ttcatcaata ggacatgata
     6181 taataaaaaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6241 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6301 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6361 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6421 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6481 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6541 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6601 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6661 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6721 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6781 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     6841 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6901 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6961 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7021 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7081 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7141 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     7201 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcctgtt
     7261 ataaaaaaag gatcaatttt gaactctctc ccaaagttga tcccttaacg atttagaaat
     7321 ccctttgaga atgtttatat acattcaagg taaccagcca actaatgaca atgattcctg
     7381 aaaaaagtaa taacaaatta ctatacagat aagttgactg atcaacttcc ataggtaaca
     7441 acctttgatc aagtaagggt atggataata aaccacctac aattgcaata cctgttccct
     7501 ctgataaaaa gctggtaaag ttaagcaaac tcattccagc accagcttcc tgctgtttca
     7561 agctacttga aacaattgtt gatataactg ttttggtgaa cgaaagccca cctaaaacaa
     7621 atacgattat aattgtcatg aaccatgatg ttgtttctaa aagaaaggaa gcagttaaaa
     7681 agctaacaga aagaaatgta actccgatgt ttaacacgta taaaggacct cttctatcaa
     7741 caagtatccc accaatgtag ccgaaaataa tgacactcat tgttccaggg aaaataatta
     7801 cacttccgat ttcggcagta cttagctggt gaacatcttt catcatataa ggaaccatag
     7861 agacaaaccc tgctactgtt ccaaatataa ttcccccaca aagaactcca atcataaaag
     7921 gtatattttt ccctaatccg ggatcaacaa aaggatctgt tactttcctg atatgtttta
     7981 caaatatcag gaatgacagc acgctaacga taagaaaaga aatgctatat gatgttgtaa
     8041 acaacataaa aaatacaatg cctacagaca ttagtataat tcctttgata tcaaaatgac
     8101 cttttatcct tacttctttc tttaataatt tcataagaaa cggaacagtg ataattgtta
     8161 tcataggaat gagtagaaga taggaccaat gaatataatg ggctatcatt ccaccaatcg
     8221 ctggaccgac tccttctccc atggctacta tcgatccaat aagaccaaat gctttacccc
     8281 tattttcctt tggaatatag cgcgcaacta caaccattac gagtgctgga aatgcagctg
     8341 caccagcccc ttgaataaaa cgagccataa taagtaagga aaagaaagaa tggccaacaa
     8401 acccaattac cgacccgaaa caatttatta taattccaaa taggagtaac cttttgatgc
     8461 ctaattgatc agatagcttt ccatatacag ctgttccaat ggaaaaggtt aacataaagg
     8521 ctgtgttcac ccagtttgta ctcgcaggtg gtttattaaa atcatttgca atatcaggta
     8581 atgagacgtt caaaaccatt tcatttaata cgctaaaaaa agataaaatg caaagccaaa
     8641 ttaaaatttg gttgtgtcgt aaattcgatt gtgaatagga tgtattcaca tttcaccctc
     8701 caataatgag ggcagacgta gtttataggg ttaatgatac gcttccctct tttaattgaa
     8761 ccctgttaca ttcattacac ttcataatta attcctccta aacttgatta aaacatttta
     8821 ccacatataa actaagtttt aaattcagta tttcatcact tatacaacaa tatggcccgt
     8881 ttgttgaact actctttaat aaaataattt ttccgttccc aattccacat tgcaataata
     8941 gaaaatccat cttcatcggc tttttcgtca tcatctgtat gaatcaaatc gccttcttct
     9001 gtgtcatcaa ggtttaattt tttatgtatt tcttttaaca aaccaccata ggagattaac
     9061 cttttacggt gtaaaccttc ctccaaatca gacaaacgtt tcaaattctt ttcttcatca
     9121 tcggtcataa aatccgtatc ctttacagga tattttgcag tttcgtcaat tgccgattgt
     9181 atatccgatt tatatttatt tttcggtcga atcatttgaa cttttacatt tggatcatag
     9241 tctaatttca ttgccttttt ccaaaattga atccattgtt tttgattcac gtagttttct
     9301 gtattcttaa aataagttgg ttccacacat accaatacat gcatgtgctg attataagaa
     9361 ttatctttat tatttattgt cacttccgtt gcacgcataa aaccaacaag atttttatta
     9421 atttttttat attgcatcat tcggcgaaat ccttgagcca tatctgacaa actcttattt
     9481 aattcttcgc catcataaac atttttaact gttaatgtga gaaacaacca acgaactgtt
     9541 ggcttttgtt taataacttc agcaacaacc ttttgtgact gaatgccatg tttcattgct
     9601 ctcctccagt tgcacattgg acaaagcctg gatttacaaa accacactcg atacaacttt
     9661 ctttcgcctg tttcacgatt ttgtttatac tctaatattt cagcacaatc ttttactctt
     9721 tcagcctttt taaattcaag aatatgcaga agttcaaagt aatcaacatt agcgattttc
     9781 ttttctctcc atggtctcac ttttccactt tttgtcttgt ccactaaaac ccttgatttt
     9841 tcatctgaat aaatgctact attaggacac ataatattaa aagaaacccc catctattta
     9901 gttatttgtt tggtcactta taactttaac agatggggtt tttctgtgca accaatttta
     9961 agggttttca atactttaaa acacatacat acca
//
