LOCUS       sequence-25485-e         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC257
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(180..227)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             303..323
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             345..365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             387..407
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             429..449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(492..520)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aactgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
       61 tcactcacaa cgatggatac gctaacaact tggaaatgaa ataagcttgc atgcctgcag
      121 gtcgactcta gaccgcggtt ttggcgcgga tccactagtt ctagcttcaa aagcgctctg
      181 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaag atccccgggt
      241 atgtttcgaa tgatactaac ataacataga acattttcag gagctcagaa aaaatgactg
      301 ctccaaagaa gaagcgtaag gtaccggcag aaaaaatgac tgctccaaag aagaagcgta
      361 aggtaccggc agaaaaaatg actgctccaa agaagaagcg taaggtaccg gcagaaaaaa
      421 tgactgctcc aaagaagaag cgtaaggtac cggtagaaaa aatgagtaaa ggagaagaac
      481 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      541 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      601 tttgcactac tggaaaacta cctgtt
//
