LOCUS       sequence-254858-        9937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the co-expression of cytoplasmic bPAC and 
            nuclear tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Fonseca JP, Weinberg ZY, Aslankoohi E, El-Samad H
  TITLE     Biphasic Response of Protein Kinase A to Cyclic Adenosine 
            Monophosphate Triggers Distinct Epithelial Phenotypes
  JOURNAL   bioRxiv 2020, 747030
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020, 747030"
COMMENT     Sequence Label: pJPF0117
FEATURES             Location/Qualifiers
     source          1..9937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1738..2248
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2167..2185
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    2303..2327
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2354..3052
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             3053..3073
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3104..3157
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4221..4247
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4253..4282
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(4315..4339)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4370..4958
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4423..4443)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4841..4852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4972..5471
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5007..5030)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5404..5423
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5492..6091
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5492..5511)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5988..6008
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6229..6462
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6534..6655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6571..6590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6625..6644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6695..6830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6850..6869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6851..6869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6879..6896)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6879..6895)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6888..6910)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7037..7492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7124..7143)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7334..7355
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7518..7622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7623..7691,7692..8483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7841..7860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8654..9242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9143..9162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9396..9413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9530..9551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9566..9571,9572..9589,9590..9596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9604..9620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9609..9631
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9628..9644
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9628..9644
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9663..9683
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9665..9683
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9711..9937
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgatatcga attccacggg
     1741 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     1801 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     1861 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     1921 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     1981 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     2041 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     2101 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     2161 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     2221 tgaccgaatc accgacctct ctccccaggg ggatccaccg gttcgtcgac tagtccagtg
     2281 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca ccatgggaac
     2341 caattcagtc gacatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     2401 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     2461 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     2521 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     2581 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     2641 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     2701 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     2761 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     2821 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac
     2881 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     2941 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     3001 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta atcccaagaa
     3061 gaagaggaag gtggaggacg cccgtacgag tactggaagc ggagagggca gaggaagtct
     3121 gctaacatgc ggtgacgtcg aggagaatcc tggacctgga tccaagcggc tggtgtacat
     3181 cagcaagatc agcggccacc tgagcctgga agagatccag cggatcggca aggtgtccat
     3241 caagaacaac cagcgggaca acatcaccgg cgtgctgctg tacctgcagg gcctgttctt
     3301 ccagatcctg gaaggcgaga acgagaaggt ggacaagctg tacaagaaaa tcctggtgga
     3361 cgaccggcac accaacatcc tgtgcctgaa aaccgagtac gacatcaccg accggatgtt
     3421 ccccaactgg gccatgaaaa ccatcaacct gaacgagaac agcgagctga tgatccagcc
     3481 catcaagagc ctgctgcaga ccatcaccca gagccaccgg gtgctggaaa agtacatgcc
     3541 cgccagagtg atctacctga tcaaccaggg catcaacccc ctgaccgtgg agccccagct
     3601 ggtcgagaag atcatcttct tcagcgacat cctggccttc agcaccctga ccgagaagct
     3661 gcccgtgaac gaggtggtca tcctggtcaa ccggtacttc agcatctgca cccggatcat
     3721 cagcgcctac ggcggcgaag tgaccaagtt catcggcgac tgcgtgatgg ccagcttcac
     3781 caaagagcag ggcgacgccg ccatccggac cagcctggac atcatctccg agctgaagca
     3841 gctgcggcac cacgtggagg ccaccaaccc cctgcacctg ctgtacaccg gcatcggcct
     3901 gagctacggc cacgtgatcg agggcaacat gggcagcagc ctgaagatgg accacaccct
     3961 gctgggggac gccgtgaacg tggccgccag gctggaagcc ctgacaagac agctgcccta
     4021 cgccctggcc tttacagccg gcgtgaagaa gtgctgccag gcccagtgga ccttcatcaa
     4081 cctgggagcc caccaggtca agggcaagca ggaagccatc gaggtctaca ccgtgaatga
     4141 ggcccagaag tactacgaca ccctgcagat cacccagctg atccggcaga ccctggaaaa
     4201 cgacaagccg cggacgtacg ctgccccccc tggagaggct gaccctgcgt acgagcaaaa
     4261 gctgatctcg gaggaggacc tgtaagaatt cgcggccgca ctcgagatat ctagacccag
     4321 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct
     4381 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4441 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4501 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4561 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4621 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4681 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4741 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4801 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4861 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4921 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg
     4981 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     5041 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     5101 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     5161 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     5221 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     5281 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     5341 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     5401 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     5461 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     5521 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5581 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5641 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5701 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5761 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5821 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5881 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5941 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6001 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6061 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     6121 cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt
     6181 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
     6241 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6361 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6481 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6661 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6841 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7021 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7081 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7141 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7201 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7261 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7321 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7381 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7441 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7501 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7861 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8161 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8761 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9661 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     9721 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     9781 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     9841 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     9901 agatattgta tttaagtgcc tagctcgata cataaac
//
