LOCUS       sequence-25486-e         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC294
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(178..225)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             302..322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             344..364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             386..406
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             428..448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(491..519)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc
       61 actcacaacg atggatacgc taacaacttg gaaatgaaat aagcttgcat gcctgcaggt
      121 cgactctaga ccgcggtttt ggcgcggatc cactagttct agcttcaaaa gcgctctgaa
      181 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat ccccggggta
      241 tgtttcgaat gatactaaca taacatagaa cattttcagg agctcagaaa aaatgactgc
      301 tccaaagaag aagcgtaagg taccggcaga aaaaatgact gctccaaaga agaagcgtaa
      361 ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg cagaaaaaat
      421 gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa atgagtaaag gagaagaact
      481 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      541 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      601 ttgcactact ggaaaactac ct
//
