LOCUS       sequence-254866-        2032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of YFP-tagged probe for active Rac1 in Dictyostelium 
            discoideum cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Marinovic M, Sostar M, Filic V, Antolovic V, Weber I
  TITLE     Quantitative imaging of Rac1 activity in Dictyostelium cells with a 
            fluorescently labelled GTPase-binding domain from DPAKa kinase.
  JOURNAL   Histochem Cell Biol. 2016 Sep;146(3):267-79. doi: 
            10.1007/s00418-016-1440-9. Epub 2016 Apr 28.
  PUBMED    27126594
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00418-016-1440-9"; journalName: "Histochem Cell Biol"; 
            date: "2016-09"; volume: "146"; issue: "3"; pages: "267-79"
COMMENT     Sequence Label: pDM358_DPAKa(GBD)-YFP
FEATURES             Location/Qualifiers
     source          1..2032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             563..1273
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(590..618)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1213..1233
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttttttttt ttyttwttty ttaaaacaaa taaattaaat taaataaaaa ataaaaatca
       61 gatctaaaaa atggagagag aagataagaa aaaagtatca tcttcatcaa cttcatcatc
      121 atcatcttca aatggtggtc tatcgtcatc aggtaaagat cataaaaaag atcatagttc
      181 tgaagagaag gagaaagaaa agaaatcatt ctttaacaaa ctatttagta aggaaaagaa
      241 agatcatcat tcatcatcca aatcaccttc atccggtagt agtggtggtg gtgaggtgga
      301 tgaaaagaaa aagaaaaagt tatcaccacg tgttggtaca ccattcaatg tgaaacacga
      361 tgttcatgtg aatttcaatg ctgatactgg tttcgaaggt ttaccaaagg aatgggaggt
      421 tttaattaaa tccaatttcc aagagccaga ggttatgcaa catcccgaag aagttttgga
      481 tgtagttaaa ttccatgctc aatatcaggg attggcttct gcacctgcaa tgcatgcacc
      541 atccattcca ttgactagta gtagtaaagg tgaagaactt ttcactggag ttgtcccaat
      601 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      661 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      721 tgttccatgg ccaacacttg tcactacttt cggttatggt ctccaatgct ttgctagata
      781 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      841 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      901 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      961 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1021 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1081 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1141 tttaccagac aatcattacc tgtcctatca atctgccctt tcgaaagatc ccaacgagaa
     1201 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac tcggcatgga
     1261 tgaactatac aaataatcta gttaaataaa taaattattt aataaataat aaaaaaacaa
     1321 attgttgtaa taatctaata ttttcttttt tttttaattt ttttttttta aatcttaata
     1381 attattaagt tattttaatt tttttttttt tttttttttt tttttttttt tttctatcaa
     1441 aaaaatcaaa tatatttaaa aaatttatta tttacagata cattttgaat ggtgaagata
     1501 aatatatgca ttagatgtaa aacagccaaa gagtatgaaa atcaaaaaga taaagcttat
     1561 cgatttcgaa aaagtaaata gcaattatta caaaattcaa tccgaatcta cccaaataaa
     1621 ttccaatgaa attgccgatt taaaaaagtt tattaaagaa gaagtcaata aaacttcttc
     1681 caaaattgat ttctttttag tttcttcaac agatgccctt tcaaatccag aaaattattc
     1741 tctcttagaa gtaaagtgta ttaattgtca ttctttgtgt caaggaaaaa atttatatat
     1801 ttcatgtaca agagatggat gtcaaaacaa tatttgctat aattgtttag gaataaacat
     1861 aaacatatat aatgttgtta ttaattctaa actttgccct ccatgtttca atgattcggt
     1921 aatcaacaag aagtgtgcca tgtgtagtaa gaacggaact aaatgtaatt tgaaccaaga
     1981 atgtaaactt catctttgtg cacagtgttc taaaaagtgt cctatacatt ct
//
