LOCUS       sequence-254869-        1321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of YFP-tagged probe for active Rac1 in Dictyostelium 
            discoideum cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Marinovic M, Sostar M, Filic V, Antolovic V, Weber I
  TITLE     Quantitative imaging of Rac1 activity in Dictyostelium cells with a 
            fluorescently labelled GTPase-binding domain from DPAKa kinase.
  JOURNAL   Histochem Cell Biol. 2016 Sep;146(3):267-79. doi: 
            10.1007/s00418-016-1440-9. Epub 2016 Apr 28.
  PUBMED    27126594
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00418-016-1440-9"; journalName: "Histochem Cell Biol"; 
            date: "2016-09"; volume: "146"; issue: "3"; pages: "267-79"
COMMENT     Sequence Label: pDM326_DPAKa(GBD)-YFP
FEATURES             Location/Qualifiers
     source          1..1321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             533..1243
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(560..588)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1183..1203
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 taaattaaat taaataaaaa ataaaaatca gatctaaaaa atggagagag aagataagaa
       61 aaaagtatca tcttcatcaa cttcatcatc atcatcttca aatggtggtc tatcgtcatc
      121 aggtaaagat cataaaaaag atcatagttc tgaagagaag gagaaagaaa agaaatcatt
      181 ctttaacaaa ctatttagta aggaaaagaa agatcatcat tcatcatcca aatcaccttc
      241 atccggtagt agtggtggtg gtgaggtgga tgaaaagaaa aagaaaaagt tatcaccacg
      301 tgttggtaca ccattcaatg tgaaacacga tgttcatgtg aatttcaatg ctgatactgg
      361 tttcgaaggt ttaccaaagg aatgggaggt tttaattaaa tccaatttcc aagagccaga
      421 ggttatgcaa catcccgaag aagttttgga tgtagttaaa ttccatgctc aatatcaggg
      481 attggcttct gcacctgcaa tgcatgcacc atccattcca ttgactagta gtagtaaagg
      541 tgaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      601 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      661 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      721 cggttatggt ctccaatgct ttgctagata cccagatcat atgaaacggc atgacttttt
      781 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      841 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      901 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      961 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     1021 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     1081 aaatactcca attggcgatg gccctgtcct tttaccagac aatcattacc tgtcctatca
     1141 atctgccctt tcgaaagatc ccaacgagaa gagagaccac atggtccttc ttgagtttgt
     1201 aacagctgct gggattacac tcggcatgga tgaactatac aaataatcta gttaaataaa
     1261 taaattattt aataaataat aaaaaaacaa attgttgtaa taatctaata ttttcttttt
     1321 t
//
