LOCUS       sequence-25487-e         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC307
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(179..226)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             303..323
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(366..394)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actgatctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat
       61 cactcacaac gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg
      121 tcgactctag accgcggttt tggcgcggat ccactagttc tagcttcaaa agcgctctga
      181 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga tccccggggt
      241 atgtttcgaa tgatactaac ataacataga acattttcag gagctcagaa aaaatgactg
      301 ctccaaagaa gaagcgtaag gtaccggtag aaaaaatgag taaaggagaa gaacttttca
      361 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      421 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      481 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
      541 attatttaaa ttttcagcca acacttgtca ctactttctc atggggcgtt caatg
//
