LOCUS       sequence-254870-        3220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of YFP-tagged IqgC in Dictyostelium discoideum cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Marinovic M, Mijanovic L, Sostar M, Vizovisek M, Junemann A, Fonovic
            M, Turk B, Weber I, Faix J, Filic V
  TITLE     IQGAP-related protein IqgC suppresses Ras signaling during 
            large-scale endocytosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1289-1298. doi: 
            10.1073/pnas.1810268116. Epub 2019 Jan 8.
  PUBMED    30622175
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1810268116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-01-22- 22"; volume: "116"; issue: "4"; pages: 
            "1289-1298"
COMMENT     Sequence Label: pDM304_YFP-IqgC
FEATURES             Location/Qualifiers
     source          1..3220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..727
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             14..727
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(44..72)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     667..687
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atcagatcca aaaatgagta aaggtgaaga acttttcact ggagttgtcc caattcttgt
       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      181 atggccaaca cttgtcacta ctttcggtta tggtctccaa tgctttgcta gatacccaga
      241 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      301 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      361 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      421 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      481 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      541 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      601 agacaatcat tacctgtcct atcaatctgc cctttcgaaa gatcccaacg agaagagaga
      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acactcggca tggatgaact
      721 atacaaatcc ggactcagat ctatgtcaaa tttaaatata aatagtcaag cacaaaattc
      781 accagatcca acaaggagat cgtttggtac tggtggtagt ggtagtacat tttcaccagg
      841 cggtagcgat tcaagaatta tgagtgtaaa tattgtcgat attattggac aacaagatca
      901 tgtaaaaagc gaaattttag caaaaaatca aaaacaatta ttcgatttaa aaagagaaat
      961 atcacaatct agtaaaaaga attttacatt agaaagggat ataaagaatt tagataaaaa
     1021 gatagcatta ttaattaaga atagaattac attggaagag attatgaata gtggtgatat
     1081 agttgaatta caaactcgaa ccgttacatt gaaggataaa tcacaaaggg atcattatgg
     1141 acaattattt tatttacttc aaaacaatac cacctacatt gcacaattgg cgaaattggt
     1201 aaaggttgga gagatcgata atctattgca aacggtgatg tttacactct atggtaatca
     1261 atatgaggag aaagaagagc atttactatt gtcaatgttt caaaaggtat tacaagagga
     1321 attcaaagag gcaacatcca ttggttcact gttacgtgcc aacacagcat tgacacgtat
     1381 gatgacaaca tacacacgaa gaggaccagg tcaacaatat ttgaaacaac gtctcaccaa
     1441 accattggct gatatcacca gcaacaagga tttgatattg gaggtgaacc catccactgt
     1501 ctatgagaat tacatcaacg agtacgagac taaaaccggc aaaccttcac cattgaaacg
     1561 taaggtatca ccagaggagt gtgctgataa tcaaaacgtt aaagatatca ttaaaccacg
     1621 tatcgataaa ctcattgaaa ttggtgacca attcttggac tccattctat cgagtgttga
     1681 cgccgttccc tatggtattc gttggatttg caaacaaatt cgtgaattgg taaaacaaaa
     1741 gttcccacaa gcaacccgtg ctcaaacttg tggtttaata ggtggtttct tcctccttcg
     1801 ttatattaat cccgccgttg tcactccaca agcattcatg ttggtcgatg ctaaactttc
     1861 aaataacacc aagagaaatc taacttttcc cgctaaaatg cttcaaaacc ttgcaaataa
     1921 tatcatgttt ggtggcgtaa aagagttctt tatggaacca ctcaatgtat ttttagatag
     1981 gaacaaggat cgtttaaatg actttttgga atccctcact gacgtagagt ctttgagtga
     2041 tcaccttcaa ttagataaat atttagcaat ggcaaaaact tccgatatca ccatcaatat
     2101 cactttgaat gaaatgtact ttgttcattc cctattggag caacatatca atagtttggc
     2161 acccgatcaa aatgatccac ttcgtgtgat cttaaatgat ttaggtgctg ctccaccaca
     2221 attatctcgt accgagaatg caaatgtcga attgaaactc acaaatcgtt tcgctgccga
     2281 tcatcataat ttaccaactt cctcatcaac ctcttcatct ttaaatatag gtggttcagt
     2341 tggtggtggc ggtggaggtg gtggtggtga taatacatcg ggtcaatcaa atcctgaaca
     2401 agtttacaat gaaaccaaat atttattgat cactgtactt cgttcattgg cttcaactcc
     2461 tggtaccggt gatatgggtg catctttagc agaggttgct aaacatgctg cactcaataa
     2521 gaatcaagat attgtagata acattaaaaa gatattggtc aatgtaaaga aactctcatt
     2581 ggaaggctat ttgaaaggtg aagatgataa tttcactcaa cttcgtaaag atatcgtagc
     2641 ggaattactc aactatgaaa cctatatcag taaaactgct aaagacattg aaaaacttaa
     2701 agaggtttta tcagcaattc atcaacataa tctattctta caacaacaat tggattacta
     2761 cgaaaaatat ctttcaaatg ttcgtgaaaa ttgtggtacc aatgcaagtg gtagtagtga
     2821 taagaaatca aagaaggata aagataaaaa caaagataaa gactcttcca ctgataaagc
     2881 aactggaaag aaagctatgg ttaaaaaggg tcctttcaaa tttagttaca atcaaatgga
     2941 aaaggatggc ttaatctgtc aatccgaagt accagaagat agaagatcaa acatattctt
     3001 ctcttttagt tctgaatctc ctggtatcta cgatgtcgta ttaatctata gaactaccgc
     3061 tgtctctgaa attaggttac aattagatga actcttggaa atgcaatcaa atggtcaaac
     3121 tgaatacgaa acaactttct ttaaattaaa tgttaattta ttgttatatg ttttaaataa
     3181 aacatttatt tcataaacta gttaaataaa taaattattt
//
