LOCUS       sequence-254871-        3187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  simultaneous expression of YFP-tagged IqgC and mRFP-tagged active 
            Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium 
            discoideum cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Marinovic M, Mijanovic L, Sostar M, Vizovisek M, Junemann A, Fonovic
            M, Turk B, Weber I, Faix J, Filic V
  TITLE     IQGAP-related protein IqgC suppresses Ras signaling during 
            large-scale endocytosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1289-1298. doi: 
            10.1073/pnas.1810268116. Epub 2019 Jan 8.
  PUBMED    30622175
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1810268116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-01-22- 22"; volume: "116"; issue: "4"; pages: 
            "1289-1298"
COMMENT     Sequence Label: pDM304_YFP-IqgC_mRFP-Raf1(RBD)
FEATURES             Location/Qualifiers
     source          1..3187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..716
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             3..716
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(33..61)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     656..676
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaatgagtaa aggtgaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
       61 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      121 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      181 ttgtcactac tttcggttat ggtctccaat gctttgctag atacccagat catatgaaac
      241 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      301 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      361 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      421 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      481 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      541 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaatcatt
      601 acctgtccta tcaatctgcc ctttcgaaag atcccaacga gaagagagac cacatggtcc
      661 ttcttgagtt tgtaacagct gctgggatta cactcggcat ggatgaacta tacaaatccg
      721 gactcagatc tatgtcaaat ttaaatataa atagtcaagc acaaaattca ccagatccaa
      781 caaggagatc gtttggtact ggtggtagtg gtagtacatt ttcaccaggc ggtagcgatt
      841 caagaattat gagtgtaaat attgtcgata ttattggaca acaagatcat gtaaaaagcg
      901 aaattttagc aaaaaatcaa aaacaattat tcgatttaaa aagagaaata tcacaatcta
      961 gtaaaaagaa ttttacatta gaaagggata taaagaattt agataaaaag atagcattat
     1021 taattaagaa tagaattaca ttggaagaga ttatgaatag tggtgatata gttgaattac
     1081 aaactcgaac cgttacattg aaggataaat cacaaaggga tcattatgga caattatttt
     1141 atttacttca aaacaatacc acctacattg cacaattggc gaaattggta aaggttggag
     1201 agatcgataa tctattgcaa acggtgatgt ttacactcta tggtaatcaa tatgaggaga
     1261 aagaagagca tttactattg tcaatgtttc aaaaggtatt acaagaggaa ttcaaagagg
     1321 caacatccat tggttcactg ttacgtgcca acacagcatt gacacgtatg atgacaacat
     1381 acacacgaag aggaccaggt caacaatatt tgaaacaacg tctcaccaaa ccattggctg
     1441 atatcaccag caacaaggat ttgatattgg aggtgaaccc atccactgtc tatgagaatt
     1501 acatcaacga gtacgagact aaaaccggca aaccttcacc attgaaacgt aaggtatcac
     1561 cagaggagtg tgctgataat caaaacgtta aagatatcat taaaccacgt atcgataaac
     1621 tcattgaaat tggtgaccaa ttcttggact ccattctatc gagtgttgac gccgttccct
     1681 atggtattcg ttggatttgc aaacaaattc gtgaattggt aaaacaaaag ttcccacaag
     1741 caacccgtgc tcaaacttgt ggtttaatag gtggtttctt cctccttcgt tatattaatc
     1801 ccgccgttgt cactccacaa gcattcatgt tggtcgatgc taaactttca aataacacca
     1861 agagaaatct aacttttccc gctaaaatgc ttcaaaacct tgcaaataat atcatgtttg
     1921 gtggcgtaaa agagttcttt atggaaccac tcaatgtatt tttagatagg aacaaggatc
     1981 gtttaaatga ctttttggaa tccctcactg acgtagagtc tttgagtgat caccttcaat
     2041 tagataaata tttagcaatg gcaaaaactt ccgatatcac catcaatatc actttgaatg
     2101 aaatgtactt tgttcattcc ctattggagc aacatatcaa tagtttggca cccgatcaaa
     2161 atgatccact tcgtgtgatc ttaaatgatt taggtgctgc tccaccacaa ttatctcgta
     2221 ccgagaatgc aaatgtcgaa ttgaaactca caaatcgttt cgctgccgat catcataatt
     2281 taccaacttc ctcatcaacc tcttcatctt taaatatagg tggttcagtt ggtggtggcg
     2341 gtggaggtgg tggtggtgat aatacatcgg gtcaatcaaa tcctgaacaa gtttacaatg
     2401 aaaccaaata tttattgatc actgtacttc gttcattggc ttcaactcct ggtaccggtg
     2461 atatgggtgc atctttagca gaggttgcta aacatgctgc actcaataag aatcaagata
     2521 ttgtagataa cattaaaaag atattggtca atgtaaagaa actctcattg gaaggctatt
     2581 tgaaaggtga agatgataat ttcactcaac ttcgtaaaga tatcgtagcg gaattactca
     2641 actatgaaac ctatatcagt aaaactgcta aagacattga aaaacttaaa gaggttttat
     2701 cagcaattca tcaacataat ctattcttac aacaacaatt ggattactac gaaaaatatc
     2761 tttcaaatgt tcgtgaaaat tgtggtacca atgcaagtgg tagtagtgat aagaaatcaa
     2821 agaaggataa agataaaaac aaagataaag actcttccac tgataaagca actggaaaga
     2881 aagctatggt taaaaagggt cctttcaaat ttagttacaa tcaaatggaa aaggatggct
     2941 taatctgtca atccgaagta ccagaagata gaagatcaaa catattcttc tcttttagtt
     3001 ctgaatctcc tggtatctac gatgtcgtat taatctatag aactaccgct gtctctgaaa
     3061 ttaggttaca attagatgaa ctcttggaaa tgcaatcaaa tggtcaaact gaatacgaaa
     3121 caactttctt taaattaaat gttaatttat tgttatatgt tttaaataaa acatttattt
     3181 cataaac
//
