LOCUS       sequence-25489-e         574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC258
FEATURES             Location/Qualifiers
     source          1..574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(176..223)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             299..319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             341..361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             383..403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             425..445
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(488..516)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggtcg
      121 actctagacc gcggttttgg cgcggatcca ctagttctag cttcaaaagc gctctgaagt
      181 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc ccgggtatgt
      241 ttcgaatgat actaacataa catagaacat tttcaggagc tcagaaaaaa tgactgctcc
      301 aaagaagaag cgtaaggtac cggcagaaaa aatgactgct ccaaagaaga agcgtaaggt
      361 accggcagaa aaaatgactg ctccaaagaa gaagcgtaag gtaccggcag aaaaaatgac
      421 tgctccaaag aagaagcgta aggtaccggt agaaaaaatg agtaaaggag aagaactttt
      481 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      541 tgtcagtgga gagggtgaag gtgatgcaac atac
//
