LOCUS       sequence-254907-        6228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of human ELL2 with Met133I, M1381I, M186I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: Met133I, M1381I, M186I ELL2
FEATURES             Location/Qualifiers
     source          1..6228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(419..430)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(3196..3214)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3235..3251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3235..3251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3248..3270)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3259..3275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3283..3289,3290..3307,3308..3313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3328..3349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3466..3483)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3637..4225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3717..3736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4396..5187,5188..5256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5019..5038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5257..5361)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5387..5842)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5524..5545)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5736..5755
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5969..5991
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5983..6000
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5984..6000
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6007..6026
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6007..6025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6058..6091)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gagtggaggg cggccgatga gggcggttct gaagggaaca agaccaatga gacgagcccg
       61 gggcggggcg cggggccgga gggtggggcg ggtggcggcg gtggcagtcc gaggtctggc
      121 tccgcgcagt atatgacagt acgtcagcag cgggatggcc ctagcagtgg cggcggctgc
      181 agaagcccaa gcagccgcgg ccgcagtgga ggctagagcc ggagcggcgg cggcggcggc
      241 accccgggga gtttaagatg gcggcggggg ggacaggggg cctgcgggag gagcagcgct
      301 atgggctgtc gtgcggacgg ctggggcagg acaacatcac cgtactgcat gtgaagctca
      361 ccgagacggc gatccgggcg ctcgagactt accagagcca caagaattta attccttttc
      421 gaccttcaat ccagttccaa ggactccacg ggcttgtcaa aattcccaaa aatgatcccc
      481 tcaatgaagt tcataacttt aacttttatt tgtcaaatgt gggcaaagac aaccctcagg
      541 gcagctttga ctgcatccag caaacattct ccagctctgg agcctcccag ctcaattgcc
      601 tgggatttat acaagataaa attacagtgt gtgcaacaaa cgactcgtat cagatcacac
      661 gagaaagaat cacccaggca gaggaggaat cccgcaaccg aagcacaaaa gttatcaaac
      721 ccggtggacc atatgtaggg aaaagagtgc aaattcggaa agcacctcaa gctgtttcag
      781 atacagttcc tgagaggaaa aggtcaaccc ccatcaaccc tgcaaataca attcgaaaga
      841 cacatagcag cagcaccatc tctcagaggc catacaggga cagggtgatt cacttactgg
      901 ccctgaaggc ctacaagaaa ccggagctac ttgctagact ccagaaagat ggtgtcaatc
      961 aaaaagacaa gaactccctg ggagcaattc tgcaacaggt agccaatctg aattctaagg
     1021 acctctcata taccttaaag gattatgttt ttaaagagct tcaaagagac tggcctggat
     1081 acagtgaaat agacagacgg tcattggagt cagtgctctc tagaaaacta aatccgtctc
     1141 agaatgctac aggcaccagc cgttcagaat ctcctgtatg ttctagtaga gatgctgtat
     1201 cttctcctca gaaacggctt ttggattcag agtttattga tcctttaatg aataaaaaag
     1261 cccgaatatc tcacctgacg aacagagtac caccaacact aaatggtcat ttgaatccca
     1321 ccagtgaaaa atcggctgca ggcctcccac tgccccctgc ggctgctgcc atccccaccc
     1381 ctccaccgct gccttcaacc tatctgccca tctcacatcc tcctcagatt gtaaattcta
     1441 actccaactc ccctagcact ccagaaggcc gggggactca agacctacct gttgacagtt
     1501 ttagtcaaaa cgatagtatc tatgaggacc agcaagacaa atatacctct aggacttctc
     1561 tggaaacctt accccctggt tccgttctac taaagtgtcc aaagcctatg gaagaaaacc
     1621 attcaatgtc tcacaaaaag tccaaaaaga agtctaaaaa acataaggaa aaggaccaaa
     1681 taaaaaagca cgacattgag actattgagg aaaaggagga agatcttaag agagaagagg
     1741 aaattgccaa gctaaataac tccagtccaa attccagtgg aggagttaaa gaggattgca
     1801 ctgcctccat ggaaccttca gcaattgaac tcccagatta tttgataaaa tatatcgcta
     1861 tcgtctccta tgagcaacgc cagaattata aggatgactt caatgcagag tatgatgagt
     1921 acagagcttt gcatgccagg atggagactg tagctagaag atttatcaaa ctagatgcac
     1981 aaagaaagcg cctttctcca ggctcaaaag agtatcagaa tgttcatgaa gaagtcttac
     2041 aagaatatca gaagataaag cagtctagtc ccaattacca tgaagaaaaa tacagatgtg
     2101 aatatcttca taacaagctg gctcacatca aaaggctaat aggtgaattt gaccaacagc
     2161 aagcagagtc atggtcctag aactctgctt ggaccagaag atgtgaataa acttaagctt
     2221 ggaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaancgcgta accccccatt gcaatggttg
     2281 ccttaaggtg tttgctagtt gtgtacatag tgtggttaat cattagctac actgcttccc
     2341 acttgattag agcaatggga agcatactgt ggcctaccag catctggaag tgtgtgctcg
     2401 atctgtatgt gtgcagaggt ggtgtggatg tgagcgtgca tgaaggaaaa aaagctgcta
     2461 ctcctagtag gccaaacgct caggttaaac aactgacgag tgttactgta gggtgttttt
     2521 ttgttttttt gttttttttc ttctatcaaa ttgctacttt tgttgtggaa gacaaaagca
     2581 tttccatttc aacgagtttg tcagctttat taatgttggg caaaaattga tatgtcatga
     2641 aaatgaaaca gatctatagt tttgggacaa aattataaaa tgaaatgtgt aggtaaccta
     2701 tttatatact gctataaagt attttttgaa gagagatatg caaagaagct attacctaca
     2761 taagaggtat atttaaagat tttttttttt ttcatcctgg tgccaggaat ataaaaaaga
     2821 gtggatatat ttaaccataa catactgtga ttcatcaaac agcacaaact ttcatttcat
     2881 ggagtttatc tgttgacatt gatttaaact gtcacttgtt ttatcatgtg ggaacataag
     2941 ttatgtggtc aaaaatataa ggattttgaa ttaatgttga ttcaagttgt attgtctttt
     3001 caaagtgctg ccagttgaaa agggaagcat tatgtttaca aatctgtttt gaaatgtttg
     3061 ccaaaatttt ggtagtgtct ttaataaaga tgtttgtctc cagcatccag aaaaaaaaaa
     3121 aaaaaactct ccagcgctgg atccggccat aagggcctga tccttcgagg gggggcccgg
     3181 taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     3241 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3301 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3421 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3481 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3541 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3601 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3721 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3781 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3841 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3901 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3961 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4021 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4081 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4141 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4201 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4261 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4321 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4381 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4441 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4501 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4561 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4621 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4681 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4741 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4801 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4861 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4921 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4981 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5041 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5101 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5161 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5221 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5281 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5341 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     5401 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5461 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5521 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5581 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5641 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     5701 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     5761 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     5821 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     5881 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     5941 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     6001 gaattgtaat acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgcata
     6061 acttcgtata gcatacatta tacgaagtta tggatcaggc caaatcggcc gagctcgaat
     6121 tcgtcgagag cgggataagt tggcgcgtca cctagacgtc agcctcttac ctctcggacg
     6181 tcccgtccca ggagcaaaac tctagtgact ttgggcccgc ccccgtga
//
