LOCUS       sequence-254915-        9412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transformation vector for stable expression of mitochondria 
            targeted GFP1-10 in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pLSU4mg10
FEATURES             Location/Qualifiers
     source          1..9412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             740..1381
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      1412..2119
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2328..2347)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2339..3154
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2339..3154
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2412..2431)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3240..3828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3729..3748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4074..4703
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5132..6205
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6363..6557
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             7054..8079
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag
      361 agctcatttt tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt
      421 tacaattatc gataccatgc ttcgagtagc aggtagaagg ctttcttctt cagccgctag
      481 atcttcatct accttcttta caagaagctc tttcaccgtt accgatgatt cgtctccggc
      541 aagatctcct tctccgtcac tcacctcttc gtttctcgat caaatcagag gtttctcatc
      601 taattcggtt tctcccgcac atcagttggg tttagtctca gatcttccag ccacagtggc
      661 tgctattaag aatcccagtt caaaaattgt atatgatgac tccaaccatg agcgttatcc
      721 acctggagat cctagcgcaa tgtccaaagg agaagaactg tttaccggtg ttgtgccaat
      781 tttggttgaa ctcgatggtg atgtcaacgg acataagttc tcagtgagag gcgaaggaga
      841 aggtgacgcc accattggaa aattgactct taaattcatc tgtactactg gtaaacttcc
      901 tgtaccatgg ccgactctcg taacaacgct tacgtacgga gttcagtgct tttcgagata
      961 cccagaccat atgaaaagac atgacttttt taagtcggct atgcctgaag gttacgtgca
     1021 agaaagaaca atttcgttca aagatgatgg aaaatataaa actagagcag ttgttaaatt
     1081 tgaaggagat actttggtta accgcattga actgaaagga acagatttta aagaagatgg
     1141 taatattctt ggacacaaac tcgaatacaa ttttaatagt cataacgtat acatcactgc
     1201 tgataagcaa aagaacggaa ttaaagcgaa tttcacagta cgccataatg tagaagatgg
     1261 cagtgttcaa cttgccgacc attaccaaca aaacacccct attggagacg gtccggtact
     1321 tcttcctgat aatcactacc tctcaacaca aacagtcctg agcaaagatc caaatgaaaa
     1381 aggaacaggt ggcggcggaa gttagcttgt cctgctttaa tgagatatgc gagaagccta
     1441 tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg
     1501 tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat
     1561 cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg
     1621 tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat
     1681 agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc
     1741 ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg
     1801 ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga
     1861 actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc
     1921 gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga
     1981 cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc
     2041 aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca
     2101 acatgtcgag gctcagcagg accgctacta gaattcgagc tcgggcttat ctagagagct
     2161 ctggcaggat atatgcggtt gtaattcatt tttattgtct aaatttctgt atttgtttgt
     2221 ttgcaattag atccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2281 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2341 gagccatatt caacgggaaa cgtcttgttc gaggccgcga ttaaattcca acatggatgc
     2401 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2461 ccgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2521 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2581 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2641 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2701 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2761 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2821 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2881 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2941 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3001 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3061 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3121 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3181 ctggcagagc attacgctga cttgacggga cggcggcttt gttgaatcaa ttgatcttct
     3241 tgaaatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3301 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3361 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3421 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3481 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3541 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3601 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3661 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3721 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3781 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3841 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3901 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgatcgat ctctgaagaa
     3961 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     4021 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     4081 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     4141 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     4201 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     4261 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     4321 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     4381 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     4441 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     4501 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     4561 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     4621 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     4681 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     4741 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     4801 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     4861 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     4921 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     4981 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     5041 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     5101 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     5161 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     5221 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     5281 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     5341 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     5401 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     5461 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     5521 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     5581 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     5641 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     5701 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     5761 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     5821 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagttg attggatgta
     5881 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     5941 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     6001 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     6061 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     6121 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     6181 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     6241 aggggaaaaa ggtcgaaaag atctctttcc tgtggatagc ggtcgaaaag atctctttcc
     6301 tgtggatagc acgtacattg ggaacccaaa gccgtacatt ggggtcgaaa agatctcttt
     6361 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     6421 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     6481 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     6541 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     6601 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     6661 ggccgctcaa aaatggctgg cctacggcca ggcaatctgc tagcgcgcgg acaagctagg
     6721 attggcagga tatattcagt tgtaaatgat atcgcatgct atgcagcatt tttttggtgt
     6781 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     6841 gtccagggcg aattttgcga caacatgtcg aggctcagca gctgaaaatt caaacgcgct
     6901 agtcaatgct atcaatctgt cgtgttcaca cggcatcaaa cggtcaccaa tgacgtcaat
     6961 gggcttcctt agtcaatata aaaaccaacg gctcagactt accagcggca ggtatttgta
     7021 gtacatccaa cactgccctg atctcaatga gatatgaaaa agcctgaact caccgcgacg
     7081 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     7141 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     7201 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     7261 gccgcgctcc cgattccgga agtgcttgac attggggagt ttagcgagag cctgacctat
     7321 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     7381 gctgttctac aaccggtcgc ggaggcgatg gatgcgatcg ctgcggccga tcttagccag
     7441 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     7501 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     7561 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     7621 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     7681 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     7741 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     7801 gagcggaggc atccggagct tgcaggatcg ccacgactcc gggcgtatat gctccgcatt
     7861 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     7921 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     7981 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagttctcgc cgatagtgga
     8041 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatcacc gtcgccgaca
     8101 cctaataaag tcggctaatc tatgtgattg agtgtgtctt gactttgtta ttttgcatgt
     8161 ttccaatgtc atttagtaac gaaataaacg ttatcctctt ctaaaagcag gctgtgtttt
     8221 cggcaaacat cgccacccat cgctagtttt tctaaaagtg ttctaagcgg gcctggtaat
     8281 aatctatacg agcttatatt tctaatcatt gccgaaaaat cctgtttcga aataattttg
     8341 taactctctt taatatcacc acgagcaagc ttccatggaa ggtgtgccga tgtatcacct
     8401 ctcgcaagaa ttcaagcttg gaggaattcc aatcccacaa aaatctgagc ttaacagcac
     8461 agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact actacgttgt
     8521 gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg cggcaacaaa
     8581 cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa gagcagcagc
     8641 tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca aaggagaagc
     8701 tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc aaaaagccca
     8761 ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag agatctcctt
     8821 tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa ggaagttcga
     8881 aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc tcttcaattt
     8941 cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc tcatcaagac
     9001 gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
     9061 agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat ttctcaagat
     9121 cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
     9181 agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc atggagtcta
     9241 agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag ttcatacaga
     9301 gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc
     9361 tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct at
//
