LOCUS       sequence-25492-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC382
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(179..226)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(276..304)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actgatctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat
       61 cactcacaac gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg
      121 tcgactctag accgcggttt tggcgcggat ccactagttc tagcttcaaa agcgctctga
      181 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga tccccggtag
      241 aaaaaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      301 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      361 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatgggtaa
      421 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagcca acacttgtca
      481 ctactttcgg ttatggtctt caatgcttcg ccagataccc agatcatatg aaacagcatg
      541 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      601 atgacgggaa ctacaagaca cgtaagttta aacagttcgg tactaactaa ccatacatat
      661 ttaaattttc aggtgctga
//
