LOCUS       sequence-254921-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A self-assembling split-GFP based Golden Gate vector to determine 
            protein targeting to plastids in plant cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pLaNGo11-3
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1030..1375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1634..2275
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      2306..3013
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4030..4375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(4505..4810)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4607..4626)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5130..5813)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     5728..5747
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5814..5916)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5947..5994
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6010..6057
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6073..6120
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    6828..6852
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7132..7761
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8190..9263
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9329..9523
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9585..9689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9690..10484
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10655..11243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11144..11163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11379..11403
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gaattccaat cccacaaaaa
       61 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
      121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
      181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
      241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
      301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
      361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
      421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
      481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
      541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
      601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
      661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
      721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
      781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
      841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
      901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
      961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     1021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     1081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     1141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     1201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     1261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     1321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     1381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     1441 aattacattt acaattatcg ataccatgac caccgctgtc accgccgctg tttctttccc
     1501 ctctaccaaa accacctctc tctccgcccg aagctcctcc gtcatttccc ctgacaaaat
     1561 cagctacaaa aaggttcctt tgtactacag gaatgtatct gcaactggga aaatgggacc
     1621 catcagggcc gcaatgtcca aaggagaaga actgtttacc ggtgttgtgc caattttggt
     1681 tgaactcgat ggtgatgtca acggacataa gttctcagtg agaggcgaag gagaaggtga
     1741 cgccaccatt ggaaaattga ctcttaaatt catctgtact actggtaaac ttcctgtacc
     1801 atggccgact ctcgtaacaa cgcttacgta cggagttcag tgcttttcga gatacccaga
     1861 ccatatgaaa agacatgact tttttaagtc ggctatgcct gaaggttacg tgcaagaaag
     1921 aacaatttcg ttcaaagatg atggaaaata taaaactaga gcagttgtta aatttgaagg
     1981 agatactttg gttaaccgca ttgaactgaa aggaacagat tttaaagaag atggtaatat
     2041 tcttggacac aaactcgaat acaattttaa tagtcataac gtatacatca ctgctgataa
     2101 gcaaaagaac ggaattaaag cgaatttcac agtacgccat aatgtagaag atggcagtgt
     2161 tcaacttgcc gaccattacc aacaaaacac ccctattgga gacggtccgg tacttcttcc
     2221 tgataatcac tacctctcaa cacaaacagt cctgagcaaa gatccaaatg aaaaaggaac
     2281 aggtggcggc ggaagttagc ttgtcctgct ttaatgagat atgcgagaag cctatgatcg
     2341 catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc
     2401 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt
     2461 tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat
     2521 attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg
     2581 ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga
     2641 accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag
     2701 gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg
     2761 gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta
     2821 ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct
     2881 gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca
     2941 aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt
     3001 cgaggctcag caggaccgct actagaattc gagctcggag gaattccaat cccacaaaaa
     3061 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
     3121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
     3181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
     3241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
     3301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
     3361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
     3421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
     3481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
     3541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
     3601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
     3661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
     3721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
     3781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
     3841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
     3901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     3961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     4021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     4081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     4141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     4201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     4261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     4321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     4381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     4441 aattacattt acaattatcg atacaatgtg agaccgactg gctgtgtata agggagcctg
     4501 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     4561 tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata tcggtggtca
     4621 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat
     4681 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccaggc gtgtcaataa
     4741 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
     4801 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
     4861 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
     4921 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
     4981 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
     5041 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
     5101 gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccacgcg gcgtttacgc
     5161 cccccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     5221 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     5281 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     5341 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     5401 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     5461 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     5521 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     5581 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     5641 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     5701 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     5761 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     5821 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     5881 ttatggtgaa agttggaacc tcttacgtgc cgatcaaggt ctcattcgat gggcggcaaa
     5941 ttcatgcgtg accacatggt ccttcatgag tatgtaaatg ctgctgggat tacaggtggc
     6001 tctggaggta gagatcatat ggttctccac gaatacgtta acgccgcagg catcactggc
     6061 ggtagtggag gacgcgacca tatggtacta catgaatatg tcaatgcagc cggaataacc
     6121 ggataagctt agagctttcg ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg
     6181 catcagtttc attgcgcaca caccagaatc ctactgagtt tgagtattat ggcattggga
     6241 aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt
     6301 tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt
     6361 ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg
     6421 aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc
     6481 taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta tgcttattca
     6541 ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga atacaagtat
     6601 gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca gaatccttgt
     6661 cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag ttgagtatga
     6721 aaatattttt taatgcattt tatgacttgc caattgattg acaacgcttt acgaggatgc
     6781 acatgtgacc gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat
     6841 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     6901 tgaaaaggtt tatccgttcg tccatttgta tgtgccagcc gtgcggctgc atgaaatcct
     6961 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     7021 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     7081 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     7141 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     7201 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     7261 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     7321 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     7381 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     7441 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     7501 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     7561 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     7621 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     7681 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     7741 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     7801 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     7861 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     7921 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     7981 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     8041 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     8101 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     8161 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
     8221 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     8281 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     8341 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gtcgtcgatt aggaagccgc
     8401 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     8461 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     8521 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     8581 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     8641 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     8701 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     8761 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     8821 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     8881 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     8941 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     9001 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     9061 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     9121 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     9181 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     9241 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     9301 gggaaaaagg tcgaaaaagc ttctttcctg tggatagcac gtacattggg aacccaaagc
     9361 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     9421 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     9481 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     9541 cagccgaaca gctgcaaaaa gcgcccacat caaggctccg agtgcgcgga acccctattt
     9601 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9661 tgcttcaata atattgaaaa aggaagagta tggctaaaat gagaatatca ccggaattga
     9721 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
     9781 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
     9841 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
     9901 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
     9961 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
    10021 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
    10081 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
    10141 tggccgatgt ggattgcgaa aactgggaag aggacactcc atttaaagat ccgcgcgagc
    10201 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
    10261 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
    10321 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg ctcagggagg
    10381 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
    10441 gggagaaaat aaaatattat attttactgg atgaattgtt ttagctgtca gaccaagttt
    10501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    10561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10801 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    11041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    11101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    11161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctgctcg
    11281 gatctgttgg accggacagt agtcatggtt gatgggctgc ctgtatcgag tggtgatttt
    11341 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
    11401 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt
    11461 tgaacactct
//
