LOCUS       sequence-254931-        8760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dominant negative mutant mouse FGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Bordignon P, Bottoni G, Xu X, Popescu AS, Truan Z, Guenova E, Kofler
            L, Jafari P, Ostano P, Rocken M, Neel V, Dotto GP
  TITLE     Dualism of FGF and TGF-beta Signaling in Heterogeneous 
            Cancer-Associated Fibroblast Activation with ETV1 as a Critical 
            Determinant.
  JOURNAL   Cell Rep. 2019 Aug 27;28(9):2358-2372.e6. doi: 
            10.1016/j.celrep.2019.07.092.
  PUBMED    31461652
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-27- 27"; volume: "28"; issue: "9"; pages: "2358-2372.e6"
COMMENT     Sequence Label: pLenti-CMV-Blast-DNFGFR1-HA
FEATURES             Location/Qualifiers
     source          1..8760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2326..2350
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3373..3399
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3425..3449)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3480..4068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3533..3553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3951..3962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4119..4139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4124..4453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4304..4439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4501..4548
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4567..4965
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5052..5285
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5357..5478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5394..5413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5448..5467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5518..5653
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5673..5692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5674..5692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5702..5719)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5702..5718)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5711..5733)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5860..6315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5947..5966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6157..6178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6341..6445
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6446..6514,6515..7306)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6664..6683)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7477..8065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7966..7985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8219..8236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8353..8374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8389..8394,8395..8412,8413..8419)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8427..8443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8432..8454
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8451..8467
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8451..8467
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8486..8506
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8488..8506
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8534..8760
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa
     2341 aaagcaggct ccgcggccgc ccccttcacc atgtggggct ggaagtgcct cctcttctgg
     2401 gctgtgctgg tcacagccac tctctgcact gccaggccag ccccaacctt gcccgaacaa
     2461 gatgcactcc catcctcgga agatgatgac gacgacgatg actcctcctc ggaggagaaa
     2521 gagacggaca acaccaaacc aaaccgtagg cctgtagctc cctactggac atccccagag
     2581 aaaatggaga agaaactgca tgcggtgccc gctgccaaga cggtgaagtt caagtgcccg
     2641 tcgagtggga cacccaaccc cactctgcgc tggttgaaaa atggcaaaga gtttaagcct
     2701 gaccaccgaa ttggaggcta caaggttcgc tatgccacct ggagcatcat aatggattct
     2761 gtggtgcctt ctgacaaggg caactacacc tgcatcgtgg agaatgagta tgggagcatc
     2821 aaccacacct accagcttga cgtcgtggaa cgatctccgc accgacccat ccttcaggca
     2881 gggctgcctg ccaacaagac agtggccctg ggcagcaatg tggagttcat gtgtaaggtg
     2941 tacagcgatc cgcagcctca cattcagtgg ctgaagcaca tcgaggtgaa cgggagtaag
     3001 atcgggccag acaacttgcc gtatgtccag atcctgaaga ctgctggagt taataccacc
     3061 gacaaggaaa tggaggtgct tcatctacgg aatgtctcct ttgaggatgc gggggagtat
     3121 acgtgcttgg cgggtaactc tatcggactc tcccatcact ctgcatggtt gaccgttctg
     3181 gaagccctgg aagagagacc agctgtgatg acctcaccgc tctacctgga gatcattatc
     3241 tactgcaccg gggccttcct gatctcctgc atgttgggct ctgtcatcat ctataagatg
     3301 aagagcggca ccaagaagag cgacttccat agccagatgg ctgtgcacaa gctggccaag
     3361 agcatccctc tgtacccata cgatgttcca gattacgctt aatgatagaa gggtgggcgc
     3421 gccgacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca
     3481 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3541 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3601 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3661 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3721 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3781 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3841 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3901 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3961 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4021 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctgc
     4081 agatatccgg ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc agttagggtg
     4141 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4201 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4261 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4321 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4381 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4441 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     4501 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     4561 taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     4621 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     4681 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     4741 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     4801 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     4861 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     4921 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcaca attcgagctc
     4981 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5041 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
     5101 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5161 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5221 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5281 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     5341 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5461 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5521 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5581 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5641 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     5701 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5761 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     5821 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     5881 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5941 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6001 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6061 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6121 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6181 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6241 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6301 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6361 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6421 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6481 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6541 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6601 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6661 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6721 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6781 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6841 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6901 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6961 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7021 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7081 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7141 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7201 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7261 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7321 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7381 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7621 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8161 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8221 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8281 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8341 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8401 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8461 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     8521 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     8581 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     8641 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     8701 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
//
