LOCUS       sequence-25496-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC155
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(179..226)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    264..387
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(530..549)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 actgatctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat
       61 cactcacaac gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg
      121 tcgactctag accgcggttt tggcgcggat ccactagttc tagcttcaaa agcgctctga
      181 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga tccccgggta
      241 ccgagctcat tacgccaagc tatcaacttt gtatagaaaa gttgaacgag aaacgtaaaa
      301 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      361 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc
      421 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
      481 ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg
      541 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca c
//
