LOCUS       sequence-25497-e         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC244
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(183..230)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    305..428
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(571..590)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tttaactgat cttactaact aactattctc atttaaattt tcagagctta aaaatggctg
       61 aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct tgcatgcctg
      121 caggtcgact ctagaccgcg gttttggcgc ggatccacta gttctagctt caaaagcgct
      181 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aagatccccg
      241 ggtatgtttc gaatgatact aacataacat agaacatttt caggagctca ttacgccaag
      301 ctatcaactt tgtatagaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata
      361 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      421 tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt atattcccca
      481 gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc
      541 ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc
      601 atccccaa
//
