LOCUS       sequence-254971-        9342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of APEX2 in the nucleolus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang 
            HY, Ting AY
  TITLE     Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
  JOURNAL   Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027.
  PUBMED    31230715
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027."
COMMENT     Sequence Label: GFP-APEX2-NIK3x
FEATURES             Location/Qualifiers
     source          1..9342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(203..222)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     550..571
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             635..1381
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(1512..1536)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1538..1579
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1621..2209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1674..1694)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2092..2103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2281..2514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2592..2713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2629..2648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2683..2702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2753..2888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2908..2927)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2909..2927)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2937..2954)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2937..2953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2946..2968)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3095..3550
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3182..3201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3392..3413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3576..3680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3681..3749,3750..4541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3899..3918)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4712..5300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5201..5220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5454..5471
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5588..5609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5624..5629,5630..5647,5648..5654)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5662..5678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5667..5689
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5686..5702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5686..5702
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5721..5741
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5723..5741
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5769..5995
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5996..6176
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6223..6348
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6841..7074
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7259..7303
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7478..7978
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7897..7915
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7990..8385
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8445..8562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8679..8982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8983..9186
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9137..9157
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    9209..9233
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9239..9241,9242..9244,9245..9342,1..613)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9239..9241,9242..9244,9245..9342,1..613)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9284..9305)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
       61 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      121 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      181 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      241 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      301 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
      361 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      421 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      481 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
      541 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      601 cgagctgtac aagtccggac tcagatctcg agctggaaag tcttacccaa ctgtgagtgc
      661 tgattaccag gacgccgttg agaaggcgaa gaagaagctc agaggcttca tcgctgagaa
      721 gagatgcgct cctctaatgc tccgtttggc attccactct gctggaacct ttgacaaggg
      781 cacgaagacc ggtggaccct tcggaaccat caagcaccct gccgaactgg ctcacagcgc
      841 taacaacggt cttgacatcg ctgttaggct tttggagcca ctcaaggcgg agttccctat
      901 tttgagctac gccgatttct accagttggc tggcgttgtt gccgttgagg tcacgggtgg
      961 acctaaggtt ccattccacc ctggaagaga ggacaagcct gagccaccac cagagggtcg
     1021 cttgcctgat cccactaagg gttctgacca tttgagagat gtgtttggca aagctatggg
     1081 gcttactgac caagatatcg ttgctctatc tgggggtcac actattggag ctgcacacaa
     1141 ggagcgttct ggatttgagg gtccctggac ctctaatcct cttattttcg acaactcata
     1201 cttcacggag ttgttgagtg gtgagaagga aggtctcctt cagctacctt ctgacaaggc
     1261 tcttttgtct gaccctgtat tccgccctct cgttgataaa tatgcagcgg acgaagatgc
     1321 cttctttgct gattacgctg aggctcacca aaagctttcc gagcttgggt ttgctgatgc
     1381 ctcgaattca agaatgagaa agaagcgaaa aaagaagctt cgaatcctgc gcatgagaaa
     1441 gaagcgcaag aagaagctcc gcatcctcag gatgcgcaag aaacgcaaga agaagctgcg
     1501 catcctgtga gacccagctt tcttgtacaa agtggttggt aagcctatcc ctaaccctct
     1561 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac
     1621 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1681 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     1741 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     1801 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     1861 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     1921 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     1981 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2041 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2101 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2161 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     2221 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     2281 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     2341 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     2401 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     2461 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     2521 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     2581 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2701 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     2761 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2821 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2881 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     2941 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     3001 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     3061 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     3121 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3181 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3241 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3301 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3361 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3421 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3481 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3541 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3601 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3661 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3721 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3781 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3841 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3901 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3961 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4021 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4081 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4141 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4201 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4261 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4321 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4381 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4441 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4501 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4561 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4621 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4921 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4981 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5041 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5101 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5161 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5221 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5281 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5341 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5401 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5461 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5521 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5581 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5641 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5701 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     5761 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     5821 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     5881 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     5941 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     6001 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6061 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6121 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     6181 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     6241 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     6301 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     6361 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     6421 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     6481 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     6541 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     6601 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     6661 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     6721 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     6781 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     6841 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     6901 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     6961 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     7021 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     7081 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     7141 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     7201 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     7261 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     7321 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     7381 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     7441 atcgtttcag acccacctcc caaccccgag gggacccttg cgccttttcc aaggcagccc
     7501 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
     7561 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
     7621 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
     7681 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
     7741 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
     7801 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
     7861 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
     7921 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg
     7981 ggtaccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     8041 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     8101 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     8161 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     8221 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     8281 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     8341 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggcctgca gctgcagtag
     8401 taagaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     8461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     8521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     8581 agcagagatc cactttggcg ccggctcgag ggggcccggg gttattaata gtaatcaatt
     8641 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8701 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8761 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8821 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8881 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8941 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     9001 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     9061 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     9121 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     9181 agagctctct ggctaactgt cgggatcaac aagtttgtac aaaaaagcag gcttcaccat
     9241 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     9301 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc ga
//
