LOCUS       sequence-254972-       10568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  APEX2 fused to the mitochondrial nucleoid protein Twinkle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Han S, Udeshi ND, Deerinck TJ, Svinkina T, Ellisman MH, Carr SA, 
            Ting AY
  TITLE     Proximity Biotinylation as a Method for Mapping Proteins Associated 
            with mtDNA in Living Cells.
  JOURNAL   Cell Chem Biol. 2017 Mar 16;24(3):404-414. doi: 
            10.1016/j.chembiol.2017.02.002. Epub 2017 Feb 23.
  PUBMED    28238724
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2017.02.002"; journalName: "Cell Chem Biol"; 
            date: "2017-03-16- 16"; volume: "24"; issue: "3"; pages: "404-414"
COMMENT     Sequence Label: Twinkle-APEX2-V5
FEATURES             Location/Qualifiers
     source          1..10568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1710..2210
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2129..2147
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2222..2617
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2677..2794
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2911..3214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3215..3418
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3369..3389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3441..3465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5571..6317
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             6318..6359
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(6364..6388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6421..7009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6474..6494)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6892..6903)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7081..7314
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7392..7513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7429..7448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7483..7502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7553..7688
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7708..7727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7709..7727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7737..7754)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7737..7753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7746..7768)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7895..8350
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7982..8001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8192..8213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8376..8480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8481..8549,8550..9341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8699..8718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9512..10100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10001..10020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10254..10271
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10388..10409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10424..10429,10430..10447,10448..10454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10462..10478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10467..10489
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10486..10502
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10486..10502
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10521..10541
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10523..10541
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     1741 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     1801 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     1861 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     1921 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     1981 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2041 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2101 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     2161 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     2221 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     2281 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     2341 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     2401 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     2461 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     2521 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     2581 tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt
     2641 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2701 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2761 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2821 tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc
     2881 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2941 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3001 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3061 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3121 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3181 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3241 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3301 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3361 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     3421 ctggctaact gtcgggatca acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa
     3481 ccatgtgggt cctcctccga agtgggtacc ccctccgtat cttgttaccc ctgcgtgggg
     3541 agtggatggg tcggaggggc ctgccccgaa acttggcccc aggccctcct cgcagacgtt
     3601 acaggaagga gactctccaa gccttggata tgccagtgtt gcctgtaact gcaactgaaa
     3661 tccgccagta tttgcggggg catgggatcc ccttccagga tggtcacagt tgcctgcggg
     3721 cactgagccc ctttgcagag tcttcacagc tcaaaggcca gactggtgtt accacttcct
     3781 tcagcctctt cattgacaag accacaggcc actttctctg catgaccagc ctagcagaag
     3841 ggagctggga agacttccag gccagcgtgg aggggcgagg ggatggggcc agggaggggt
     3901 ttctgcttag caaggcacca gaatttgagg acagcgagga ggtccggagg atctggaacc
     3961 gagcaatacc tctctgggag ctgcctgatc aggaggaggt tcagctggct gatacaatgt
     4021 ttggccttac caaggttaca gatgacacac tcaagcgttt cagtgtgcga tatctgcgac
     4081 ctgctcgcag tcttgtcttc ccttggttct cccctggggg ctcaggatta cgaggcctga
     4141 agctcctaga ggctaaatgc cagggggatg gagtgagcta cgaggaaacc actattcccc
     4201 gacccagcgc ctaccacaat ctgtttggat taccactgat tagtcgtcga gatgctgagg
     4261 tggtactgac gagtcgtgag cttgacagcc tggccttgaa ccagtccacg gggctgccta
     4321 cccttactct accccgagga acgacctgct taccccctgc cttactccct tacctggaac
     4381 agttccggcg gattgtattc tggttggggg atgaccttcg gtcctgggaa gccgccaagt
     4441 tgtttgcacg aaaactgaac cccaaacgat gcttcttggt gcgaccagga gaccagcaac
     4501 cccgtcccct ggaggccctg aacggaggct tcaatctttc tcgtattctt cgtaccgccc
     4561 tgcctgcctg gcacaagtcc atcgtatctt tccggcagct tcgggaggag gtgctaggag
     4621 aactgtcaaa tgtggagcaa gcagctggcc tccgctggag ccgctttcca gacctcaatc
     4681 gtatcttgaa gggacatcga aagggcgagc tgacggtctt cacagggcca acaggcagtg
     4741 gaaagacgac attcatcagt gagtatgccc tggatttgtg ttcccagggg gtgaacacac
     4801 tgtggggtag cttcgagatc agcaatgtga gactagcccg ggtcatgctg acacagtttg
     4861 ccgaggggcg gctggaagat caactggaca aatatgatca ctgggctgac cgctttgagg
     4921 acctgcccct ctatttcatg actttccatg gacagcaaag catcaggact gtaatagata
     4981 caatgcaaca tgcagtctac gtctatgaca tttgtcatgt gatcatcgac aacctgcagt
     5041 tcatgatggg tcacgagcag ctgtccacag acaggatcgc agctcaagac tacatcatcg
     5101 gggtctttcg gaagtttgca acagacaata actgccatgt gacactggtc attcaccccc
     5161 ggaaagagga tgatgacaag gaactgcaga cagcgtccat ttttggctca gccaaagcaa
     5221 gccaggaagc agacaatgtt ctgatcctgc aggacaggaa gctggtaacc gggccaggga
     5281 aacggtatct gcaggtgtcc aagaaccgct ttgatggaga tgtaggtgtc ttcccgcttg
     5341 agttcaacaa gaactccctc accttctcca ttccaccaaa gaacaaggcc cggctcaaga
     5401 agatcaagga tgacactgga ccagtggcca aaaagccctc ttctggcaaa aagggggcta
     5461 cgacacagaa ctctgagatt tgctcaggcc aggcccccac tcccgaccag ccagacacct
     5521 ccaagcgttc aaagggatcc aagggctcgg gctcgacctc gggctcgggc ggaaagtctt
     5581 acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag
     5641 gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg
     5701 gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg
     5761 aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca
     5821 aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg
     5881 ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc
     5941 caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt
     6001 ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta
     6061 ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta
     6121 ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc
     6181 taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gacaaatatg
     6241 cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc
     6301 ttgggtttgc tgatgccggc aagcccatcc ccaaccccct gctgggcctg gacagcacct
     6361 aagacccagc tttcttgtac aaagtggtgc tagcgctaac cggtggcgcg ttaagtcgac
     6421 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6481 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6541 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6601 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6661 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6721 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6781 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6841 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6901 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6961 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     7021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7081 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     7321 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7381 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7501 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7561 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7621 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7681 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7741 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7801 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7861 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7921 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7981 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8041 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8101 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8161 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8221 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8281 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8341 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8401 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8461 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8521 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8581 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8641 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8701 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8761 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8821 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8881 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8941 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9001 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9061 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9121 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9181 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9241 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9301 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9361 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9421 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9481 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9541 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9601 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9661 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9721 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9841 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9901 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9961 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10021 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10081 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10141 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10201 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10261 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10321 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    10381 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    10441 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    10501 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    10561 aagcttaa
//
