LOCUS       sequence-254974-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of APEX2 at nuclear lamina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang 
            HY, Ting AY
  TITLE     Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
  JOURNAL   Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027.
  PUBMED    31230715
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027."
COMMENT     Sequence Label: V5-APEX2-LMNA
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             576..617
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             618..1364
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             1383..1412
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3440..4028
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3493..3513)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3911..3922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4100..4333
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4411..4532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4448..4467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4502..4521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4572..4707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4727..4746)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4728..4746)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4756..4773)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4756..4772)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4765..4787)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4914..5369
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5001..5020)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5211..5232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5395..5499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5500..5568,5569..6360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5718..5737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6531..7119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7020..7039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7273..7290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7407..7428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7443..7448,7449..7466,7467..7473)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7481..7497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7486..7508
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7505..7521
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7505..7521
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7540..7560
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7542..7560
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7588..7814
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7815..7995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8042..8167
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8660..8893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9078..9122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9297..9797
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9716..9734
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9809..10204
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10264..10381
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggcttcgaa ggagatagaa ccatgggcaa gcccatcccc aaccccctgc
      601 tgggcctgga cagcaccgga aagtcttacc caactgtgag tgctgattac caggacgccg
      661 ttgagaaggc gaagaagaag ctcagaggct tcatcgctga gaagagatgc gctcctctaa
      721 tgctccgttt ggcattccac tctgctggaa cctttgacaa gggcacgaag accggtggac
      781 ccttcggaac catcaagcac cctgccgaac tggctcacag cgctaacaac ggtcttgaca
      841 tcgctgttag gcttttggag ccactcaagg cggagttccc tattttgagc tacgccgatt
      901 tctaccagtt ggctggcgtt gttgccgttg aggtcacggg tggacctaag gttccattcc
      961 accctggaag agaggacaag cctgagccac caccagaggg tcgcttgcct gatcccacta
     1021 agggttctga ccatttgaga gatgtgtttg gcaaagctat ggggcttact gaccaagata
     1081 tcgttgctct atctgggggt cacactattg gagctgcaca caaggagcgt tctggatttg
     1141 agggtccctg gacctctaat cctcttattt tcgacaactc atacttcacg gagttgttga
     1201 gtggtgagaa ggaaggtctc cttcagctac cttctgacaa ggctcttttg tctgaccctg
     1261 tattccgccc tctcgttgat aaatatgcag cggacgaaga tgccttcttt gctgattacg
     1321 ctgaggctca ccaaaagctt tccgagcttg ggtttgctga tgcctccgga ctcagatcta
     1381 tggagcaaaa gctcatttct gaagaggact tgaatgagac cccgtcccag cggcgcgcca
     1441 cccgcagcgg ggcgcaggcc agctccactc cgctgtcgcc cacccgcatc acccggctgc
     1501 aggagaagga ggacctgcag gagctcaatg atcgcttggc ggtctacatc gaccgtgtgc
     1561 gctcgctgga aacggagaac gcagggctgc gccttcgcat caccgagtct gaagaggtgg
     1621 tcagccgcga ggtgtccggc atcaaggccg cctacgaggc cgagctcggg gatgcccgca
     1681 agacccttga ctcagtagcc aaggagcgcg cccgcctgca gctggagctg agcaaagtgc
     1741 gtgaggagtt taaggagctg aaagcgcgca ataccaagaa ggagggtgac ctgatagctg
     1801 ctcaggctcg gctgaaggac ctggaggctc tgctgaactc caaggaggcc gcactgagca
     1861 ctgctctcag tgagaagcgc acgctggagg gcgagctgca tgatctgcgg ggccaggtgg
     1921 ccaagcttga ggcagcccta ggtgaggcca agaagcaact tcaggatgag atgctgcggc
     1981 gggtggatgc tgagaacagg ctgcagacca tgaaggagga actggacttc cagaagaaca
     2041 tctacagtga ggagctgcgt gagaccaagc gccgtcatga gacccgactg gtggagattg
     2101 acaatgggaa gcagcgtgag tttgagagcc ggctggcgga tgcgctgcag gaactgcggg
     2161 cccagcatga ggaccaggtg gagcagtata agaaggagct ggagaagact tattctgcca
     2221 agctggacaa tgccaggcag tctgctgaga ggaacagcaa cctggtgggg gctgcccacg
     2281 aggagctgca gcagtcgcgc atccgcatcg acagcctctc tgcccagctc agccagctcc
     2341 agaagcagct ggcagccaag gaggcgaagc ttcgagacct ggaggactca ctggcccgtg
     2401 agcgggacac cagccggcgg ctgctggcgg aaaaggagcg ggagatggcc gagatgcggg
     2461 caaggatgca gcagcagctg gacgagtacc aggagcttct ggacatcaag ctggccctgg
     2521 acatggagat ccacgcctac cgcaagctct tggagggcga ggaggagagg ctacgcctgt
     2581 cccccagccc tacctcgcag cgcagccgtg gccgtgcttc ctctcactca tcccagacac
     2641 agggtggggg cagcgtcacc aaaaagcgca aactggagtc cactgagagc cgcagcagct
     2701 tctcacagca cgcacgcact agcgggcgcg tggccgtgga ggaggtggat gaggagggca
     2761 agtttgtccg gctgcgcaac aagtccaatg aggaccagtc catgggcaat tggcagatca
     2821 agcgccagaa tggagatgat cccttgctga cttaccggtt cccaccaaag ttcaccctga
     2881 aggctgggca ggtggtgacg atctgggctg caggagctgg ggccacccac agccccccta
     2941 ccgacctggt gtggaaggca cagaacacct ggggctgcgg gaacagcctg cgtacggctc
     3001 tcatcaactc cactggggaa gaagtggcca tgcgcaagct ggtgcgctca gtgactgtgg
     3061 ttgaggacga cgaggatgag gatggagatg acctgctcca tcaccaccac ggctcccact
     3121 gcagcagctc gggggacccc gctgagtaca acctgcgctc gcgcaccgtg ctgtgcggga
     3181 cctgcgggca gcctgccgac aaggcatctg ccagcggctc aggagcccag gtgggcggac
     3241 ccatctcctc tggctcttct gcctccagtg tcacggtcac tcgcagctac cgcagtgtgg
     3301 ggggcagtgg gggtggcagc ttcggggaca atctggtcac ccgctcctac ctcctgggca
     3361 actccagccc ccgaacccag agcccccaga actgcagcat catgtaagct agcgctaacc
     3421 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4021 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4081 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4141 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4201 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4261 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4321 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     4381 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4441 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4501 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4561 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4621 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4681 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4741 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4801 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4861 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4921 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4981 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5041 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5101 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5161 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5221 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5281 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5341 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5401 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5461 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5521 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5581 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5641 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5701 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5761 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5821 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5881 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5941 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6001 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6061 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6121 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6181 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6241 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6301 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6361 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6421 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6481 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6661 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6901 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7141 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7201 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7261 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7321 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7381 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7441 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7501 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7561 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7621 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7681 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7741 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7801 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7861 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7921 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7981 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8041 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8101 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8161 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8221 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8281 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8341 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8401 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8461 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8521 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8581 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8641 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8701 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8761 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8821 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8881 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8941 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9001 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9061 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9121 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9181 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9241 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc
     9301 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     9361 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     9421 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     9481 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     9541 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     9601 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     9661 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     9721 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     9781 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     9841 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     9901 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     9961 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
    10021 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
    10081 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
    10141 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
    10201 gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag tattcatcca
    10261 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    10321 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    10381 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggg
    10441 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg
//
