LOCUS       sequence-254976-       10263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of APEX2 at nuclear pore.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang 
            HY, Ting AY
  TITLE     Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
  JOURNAL   Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027.
  PUBMED    31230715
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027."
COMMENT     Sequence Label: V5-APEX2-SENP2
FEATURES             Location/Qualifiers
     source          1..10263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     371..388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    505..526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(541..546,547..564,565..571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    579..595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     584..606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     603..619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     603..619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     638..658
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        640..658
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        686..912
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             913..1093
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1140..1265
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1758..1991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2176..2220
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2395..2895
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2814..2832
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2907..3302
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3362..3479
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3596..3899
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3900..4103
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4054..4074
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4126..4150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4171..4212
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4213..4959
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     misc_feature    6801..7389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6854..6874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7272..7283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7461..7694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7772..7893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7809..7828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7863..7882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7933..8068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8088..8107)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8089..8107)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8117..8134)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8117..8133)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8126..8148)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8275..8730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8362..8381)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8572..8593
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8756..8860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8861..8929,8930..9721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9079..9098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9892..10263,1..217)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      601 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
      661 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
      721 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      781 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      841 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      901 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1081 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1141 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1201 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1261 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1321 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1381 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1441 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1501 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1561 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1621 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1681 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1741 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1801 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1861 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1921 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1981 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     2041 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2101 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2161 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2221 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2281 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2341 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc
     2401 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     2461 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     2521 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     2581 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     2641 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     2701 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     2761 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     2821 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     2881 gacctctctc cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc
     2941 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     3001 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     3061 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     3121 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     3181 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     3241 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     3301 gcctgcagct gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca
     3361 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     3421 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     3481 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt
     3541 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     3601 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     3661 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     3721 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     3781 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     3841 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3901 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     3961 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     4021 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     4081 gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa
     4141 aaagcaggct tcgaaggaga tagaaccatg ggcaagccca tccccaaccc cctgctgggc
     4201 ctggacagca ccggaaagtc ttacccaact gtgagtgctg attaccagga cgccgttgag
     4261 aaggcgaaga agaagctcag aggcttcatc gctgagaaga gatgcgctcc tctaatgctc
     4321 cgtttggcat tccactctgc tggaaccttt gacaagggca cgaagaccgg tggacccttc
     4381 ggaaccatca agcaccctgc cgaactggct cacagcgcta acaacggtct tgacatcgct
     4441 gttaggcttt tggagccact caaggcggag ttccctattt tgagctacgc cgatttctac
     4501 cagttggctg gcgttgttgc cgttgaggtc acgggtggac ctaaggttcc attccaccct
     4561 ggaagagagg acaagcctga gccaccacca gagggtcgct tgcctgatcc cactaagggt
     4621 tctgaccatt tgagagatgt gtttggcaaa gctatggggc ttactgacca agatatcgtt
     4681 gctctatctg ggggtcacac tattggagct gcacacaagg agcgttctgg atttgagggt
     4741 ccctggacct ctaatcctct tattttcgac aactcatact tcacggagtt gttgagtggt
     4801 gagaaggaag gtctccttca gctaccttct gacaaggctc ttttgtctga ccctgtattc
     4861 cgccctctcg ttgataaata tgcagcggac gaagatgcct tctttgctga ttacgctgag
     4921 gctcaccaaa agctttccga gcttgggttt gctgatgcct ccggccggac tcagatctcg
     4981 agctcaagct tcgaattcat gtacagatgg ctggttagga ttctcggcac cattttccgt
     5041 ttctgcgacc ggtcggtgcc ccctgcccgg gccctcctga agaggcggcg ctcagacagc
     5101 actctgtttt ctacagtgga cactgatgaa ataccagcca aaagaccaag attagattgc
     5161 tttattcacc aagtgaaaaa cagtctctac aatgctgcca gcttatttgg attcccattc
     5221 cagctgacca caaagcccat ggtaacttct gcttgtaatg gaacacggaa tgtggcccct
     5281 tcaggagagg tattttcgaa ctcttcatct tgtgaactga caggttctgg atcctggaac
     5341 aacatgctga aactgggtaa taaatctcct aatggaataa gtgactatcc aaagatcaga
     5401 gtgacagtta cccgagatca gccacgcaga gtcctgcctt cctttggttt tactttgaac
     5461 tcagaaggct gtaatagaag accaggtggc cgtcgccata gcaaaggtaa tccagagagt
     5521 tctttaatgt ggaaacctca ggaacaggct gtaacagaga tgatttctga agagagtggc
     5581 aagggtctga ggcgtcccca ttgtactgtg gaggagggtg ttcaaaaaga ggaaagagag
     5641 aagtaccgaa agttattgga acgacttaaa gaaagtggtc atggaaactc tgtctgtcct
     5701 gtaacttcaa attatcacag ttctcaaaga agtcagatgg acacattaaa gaccaaaggc
     5761 tggggggaag agcaaaatca cggagtcaaa acaactcagt ttgttccaaa acaatataga
     5821 cttgttgaaa caaggggacc tctatgttca ttgagaagtg aaaagaggtg ttcaaagggg
     5881 aaaattactg atacagagac gatggtcgga atcagatttg aaaatgaaag taggagggga
     5941 taccaactgg agcctgacct atcagaagaa gtgtcggccc gactccgcct gggcagtgga
     6001 agcaatggct tactcaggag gaaagtgtca ataattgaga caaaggaaaa gaattgctca
     6061 ggcaaagaga gggacagaag aacggacgat ctccttgaac ttacagagga catggaaaag
     6121 gaaatcagta atgccctagg ccatggccca caggatgaaa tcctaagtag tgctttcaaa
     6181 ttgcgaatta ctcgaggaga tattcagaca ttaaagaact atcactggct caatgatgaa
     6241 gtcattaatt tttacatgaa tcttctggtg gaaagaaata aaaagcaagg ctatccagca
     6301 cttcatgtat tcagtacttt cttctatcct aaattaaagt ctgggggtta ccaagcagtg
     6361 aaacgatgga ccaaaggggt aaatctcttt gaacaagaaa ttattctggt gcctattcat
     6421 cggaaggtac attggagcct ggtggtgatt gacctaagaa aaaagtgtct taaatatctg
     6481 gattctatgg gacaaaaggg ccacaggatc tgtgagattc tccttcagta tttacaggat
     6541 gaaagtaaga ccaaaagaaa tagtgatctg aatcttttag agtggaccca tcacagcatg
     6601 aaaccacacg agattcctca acagctgaat gggagtgatt gtggaatgtt tacttgtaaa
     6661 tatgcagatt atatttctag ggacaaacct atcacattta ctcagcacca gatgcctctc
     6721 ttccggaaga agatggtgtg ggaaatcctt catcagcagt tgctgtgagc tagcgctaac
     6781 cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6841 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6901 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6961 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7021 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7081 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7141 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     7201 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7261 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7321 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7381 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7441 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     7501 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7681 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     7741 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7981 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8041 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     8101 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8161 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8221 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8281 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8341 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8401 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8461 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8521 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8581 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8641 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8701 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8761 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8821 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8881 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8941 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9001 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9061 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9121 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9181 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9241 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9301 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9361 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9421 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9481 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9541 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9601 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9661 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9721 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10261 act
//
