LOCUS       sequence-254985-        1211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of YFP-tagged probe for active Rac1 in Dictyostelium 
            discoideum cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Marinovic M, Sostar M, Filic V, Antolovic V, Weber I
  TITLE     Quantitative imaging of Rac1 activity in Dictyostelium cells with a 
            fluorescently labelled GTPase-binding domain from DPAKa kinase.
  JOURNAL   Histochem Cell Biol. 2016 Sep;146(3):267-79. doi: 
            10.1007/s00418-016-1440-9. Epub 2016 Apr 28.
  PUBMED    27126594
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00418-016-1440-9"; journalName: "Histochem Cell Biol"; 
            date: "2016-09"; volume: "146"; issue: "3"; pages: "267-79"
COMMENT     Sequence Label: pDM304_DPAKa(GBD)-YFP
FEATURES             Location/Qualifiers
     source          1..1211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             495..1205
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(522..550)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1145..1165
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaatggagag agaagataag aaaaaagtat catcttcatc aacttcatca tcatcatctt
       61 caaatggtgg tctatcgtca tcaggtaaag atcataaaaa agatcatagt tctgaagaga
      121 aggagaaaga aaagaaatca ttctttaaca aactatttag taaggaaaag aaagatcatc
      181 attcatcatc caaatcacct tcatccggta gtagtggtgg tggtgaggtg gatgaaaaga
      241 aaaagaaaaa gttatcacca cgtgttggta caccattcaa tgtgaaacac gatgttcatg
      301 tgaatttcaa tgctgatact ggtttcgaag gtttaccaaa ggaatgggag gttttaatta
      361 aatccaattt ccaagagcca gaggttatgc aacatcccga agaagttttg gatgtagtta
      421 aattccatgc tcaatatcag ggattggctt ctgcacctgc aatgcatgca ccatccattc
      481 cattgactag tagtagtaaa ggtgaagaac ttttcactgg agttgtccca attcttgttg
      541 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      601 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      661 ggccaacact tgtcactact ttcggttatg gtctccaatg ctttgctaga tacccagatc
      721 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      781 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      841 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      901 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      961 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1021 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1081 acaatcatta cctgtcctat caatctgccc tttcgaaaga tcccaacgag aagagagacc
     1141 acatggtcct tcttgagttt gtaacagctg ctgggattac actcggcatg gatgaactat
     1201 acaaataatc t
//
