LOCUS       sequence-25499-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC260
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(180..227)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    295..419
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aactgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
       61 tcactcacaa cgatggatac gctaacaact tggaaatgaa ataagcttgc atgcctgcag
      121 gtcgactcta gaccgcggtt ttggcgcgga tccactagtt ctagcttcaa aagcgctctg
      181 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaag atccccgggt
      241 atgtttcgaa tgatactaac ataacataga acattttcag gaggatccct cgagacaagt
      301 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
      361 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatga
      421 atcaactact tagatggtat tagtgacctg tagtcgaccg acagccttcc aaatgttctt
      481 cgggtgatgc tgccaactta gtcgaccgac agccttccaa atgttcttct caaacggaat
      541 cgccgtatcc agcctactcg ctattgtcct caatgccgta ttaaatcata aaaagaaata
      601 agaaaaagag gtgcgagcct cttttttgtg tgacaaaata aaaacatcta cctattcata
      661 tacgctagtg tcatagtcct gaaaatcatc tgcatcaaga acaatttcac aactcttata
      721 cttttctctt acaagtcgtt cggcttcatc tggattttca gcctctatac ttactaaa
//
